Berberine attenuates obesity‐induced insulin resistance by inhibiting miR‐27a secretion
Junda Du,
Yu Zhu,
Xuehan Yang
et al.
Abstract:IntroductionBerberine (BBR) is an alkaloid found in plants. It has neuroprotective, anti‐inflammatory and lipid‐lowering activity. However, the efficacy of treatment with BBR and the mechanisms through which it acts need further study.AimsThis study investigated the therapeutic effects and the mechanism of action of BBR on obesity‐induced insulin resistance in peripheral tissues.MethodsHigh‐fat‐fed C57BL/6J mice and low‐fat‐fed C57BL/6J mice with miR‐27a overexpression were given BBR intervention (100 mg/kg, p… Show more
“…FATP1 exhibits high expression levels in hormone-sensitive tissues, such as WAT, where insulin is required for FATP1 to facilitate fatty acid (FA) uptake. FATP4 facilitates FA uptake not just in insulin-sensitive tissues but also in insulin-insensitive tissues [ 48 , 49 , 50 ], as in the case of our experimental condition, in which the widely renowned model of PA-induced insulin resistance determines the reduction of FATP levels. Impaired lipid trafficking caused by PA was recovered by the OLE and COMBO treatments, which were able to restore both FATP1–4 levels compared to the PA-challenged group, suggesting their potential in maintaining basal adipocyte lipid import.…”
Obesity is increasingly prevalent worldwide and is linked to metabolic diseases, such as insulin resistance (IR) and type 2 diabetes mellitus (T2DM), due to excessive free fatty acids (FFAs). Although lifestyle changes are effective, they often prove to be insufficient as initial treatments for obesity. Additionally, while surgical and pharmacological interventions are available, they are not entirely safe or effective. Recently, interest has grown in utilizing food waste and plant-derived phenolic compounds for their health benefits, presenting a promising avenue for managing obesity and its related disorders. Indeed, many studies have examined the potential inhibitory effects of the natural extract on adipocyte differentiation and lipid accumulation. This study focused on the evaluation of the effects of standardized extracts obtained from red oranges and olive leaf waste on 3T3-L1 murine pre-adipocyte and adipocyte functionality. Red orange extract (ROE) and olive leaf extract (OLE), alone and in combination, were tested to assess their anti-obesity and anti-inflammatory effects, as well as their potential therapeutic benefits. Three in vitro models were established to investigate the effects of the extracts on (I) adipocyte differentiation; (II) mature and hypertrophic adipocytes challenged with palmitic acid (PA) and erastin (ER), respectively; and (III) erastin-induced cytotoxicity on pre-adipocytes.
“…FATP1 exhibits high expression levels in hormone-sensitive tissues, such as WAT, where insulin is required for FATP1 to facilitate fatty acid (FA) uptake. FATP4 facilitates FA uptake not just in insulin-sensitive tissues but also in insulin-insensitive tissues [ 48 , 49 , 50 ], as in the case of our experimental condition, in which the widely renowned model of PA-induced insulin resistance determines the reduction of FATP levels. Impaired lipid trafficking caused by PA was recovered by the OLE and COMBO treatments, which were able to restore both FATP1–4 levels compared to the PA-challenged group, suggesting their potential in maintaining basal adipocyte lipid import.…”
Obesity is increasingly prevalent worldwide and is linked to metabolic diseases, such as insulin resistance (IR) and type 2 diabetes mellitus (T2DM), due to excessive free fatty acids (FFAs). Although lifestyle changes are effective, they often prove to be insufficient as initial treatments for obesity. Additionally, while surgical and pharmacological interventions are available, they are not entirely safe or effective. Recently, interest has grown in utilizing food waste and plant-derived phenolic compounds for their health benefits, presenting a promising avenue for managing obesity and its related disorders. Indeed, many studies have examined the potential inhibitory effects of the natural extract on adipocyte differentiation and lipid accumulation. This study focused on the evaluation of the effects of standardized extracts obtained from red oranges and olive leaf waste on 3T3-L1 murine pre-adipocyte and adipocyte functionality. Red orange extract (ROE) and olive leaf extract (OLE), alone and in combination, were tested to assess their anti-obesity and anti-inflammatory effects, as well as their potential therapeutic benefits. Three in vitro models were established to investigate the effects of the extracts on (I) adipocyte differentiation; (II) mature and hypertrophic adipocytes challenged with palmitic acid (PA) and erastin (ER), respectively; and (III) erastin-induced cytotoxicity on pre-adipocytes.
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