Berberine associated photodynamic therapy promotes autophagy and apoptosis via ROS generation in renal carcinoma cells

Lopes, Tairine Zara; Moraes, Fábio Rogério de; Tedesco, Antônio Cláudio; Arni, R.K.; Rahal, Paula; Calmon, Marília Freitas

doi:10.1016/j.biopha.2019.109794

Cited by 67 publications

(40 citation statements)

References 77 publications

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“…*p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group (n 3). (Jin et al, 2018;Luo et al, 2019;Lopes et al, 2020), whether berberinemediated angiogenesis-associated mechanisms contributes to the chemo-sensitivity of the gastric cancer cells should be further explored.…”

Section: Discussionmentioning

confidence: 99%

Berberine Improves Chemo-Sensitivity to Cisplatin by Enhancing Cell Apoptosis and Repressing PI3K/AKT/mTOR Signaling Pathway in Gastric Cancer

Kou

Tong

et al. 2020

Front. Pharmacol.

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Gastric cancer is one of the most common malignancies ranks as the second leading cause of cancer-related mortality in the world. Cisplatin (DDP) is commonly used for gastric cancer treatment, whereas recurrence and metastasis are common because of intrinsic and acquired DDP-resistance. The aim of this study is to examine the effects of berberine on the DDP-resistance in gastric cancer and explore the underling mechanisms. In this study, we established the DDP-resistant gastric cancer cells, where the IC50 values of DDP in the BGC-823/DDP and SGC-7901/DDP were significantly higher than that in the corresponding parental cells. Berberine could concentration-dependently inhibited the cell viability of BGC-823 and SGC-7901 cells; while the inhibitory effects of berberine on the cell viability were largely attenuated in the DDP-resistant cells. Berberine pre-treatment significantly sensitized BGC-823/DDP and SGC-7901/DDP cells to DDP. Furthermore, berberine treatment concentration-dependently down-regulated the multidrug resistance-associated protein 1 and multi-drug resistance-1 protein levels in the BGC-823/DDP and SGC7901/DDP cells. Interestingly, the cell apoptosis of BGC-823/DDP and SGC-7901/DDP cells was significantly enhanced by co-treatment with berberine and DDP. The results from animals also showed that berberine treatment sensitized SGC-7901/DDP cells to DDP in vivo. Mechanistically, berberine significantly suppressed the PI3K/AKT/mTOR in the BGC-823/DDP and SGC-7901/DDP cells treated with DDP. In conclusion, we observed that berberine sensitizes gastric cancer cells to DDP. Further mechanistic findings suggested that berberine-mediated DDP-sensitivity may be associated with reduced expression of drug transporters (multi-drug resistance-1 and multidrug resistance-associated protein 1), enhanced apoptosis and repressed PI3K/AKT/mTOR signaling.

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Section: Discussionmentioning

confidence: 99%

Berberine Improves Chemo-Sensitivity to Cisplatin by Enhancing Cell Apoptosis and Repressing PI3K/AKT/mTOR Signaling Pathway in Gastric Cancer

Kou

Tong

et al. 2020

Front. Pharmacol.

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“…Interestingly, BBR treatment restricts the light source only to tumor cells and protects the HK-2 cells from damages caused by photodynamic therapy. This evidence shows that BBR exerts its photosensitive effects on tumor cells and protective effect on normal cells (Lopes et al, 2020).…”

Section: Bbr Modulates Autophagymentioning

confidence: 75%

“…These evidences show that BBR could effectively inhibit autophagy in chemoresistant tumors and augment their sensitivity to antineoplastic drugs. However, in ACHN and 786-O human renal carcinoma cells, BBR induces sensitivity to photodynamic therapy and thus inhibits tumor growth very effectively by triggering ROS induced upregulation of autophagy and apoptosis (Lopes et al, 2020). This in turn shows its dual ability to target autophagy in drug-resistant cells.…”

Section: Bbr Modulates Autophagymentioning

confidence: 99%

Berberine—A potent chemosensitizer and chemoprotector to conventional cancer therapies

Devarajan

Jayaraman

Mahendra

et al. 2021

Phytotherapy Research

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Chemotherapy and radiotherapy are mainstay treatments for cancer patients. However, their clinical outcomes are highly limited by the resistance of malignant tumors to these therapies and the incurrence of serious damages in vital organs. This in turn necessitates the development of adjunct drugs that overcomes chemo/radioresistance in refractory cancers and protects vital organs from the cytotoxic effects of cancer therapies. In recent years, Berberine (BBR), a natural isoquinoline alkaloid has garnered more attention due to its potent chemosensitizing and chemoprotective properties. BBR effectively sensitizes refractory cancers to chemotherapy and radiotherapy by ameliorating the diverse events underlying therapy resistance. Furthermore, it protects the heart, liver, lungs, and kidneys from severe damages caused by these therapies. In this review, we discuss the molecular mechanisms underlying the chemo/radiosensitizing and chemo/radioprotective potential of BBR during cancer treatment. Also, we highlight the limitations that hamper the clinical application of BBR as an adjunct drug and how novel innovations have been made in recent years to circumvent these challenges.

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“…In addition, we don't clear whether there is any Sirt3independent mechanism for autophagy regulation. Others' research clarifies that berberine associated photodynamic therapy promotes autophagy via ROS generation [60]. In addition, mitochondrial E3 ubiquitin ligase 1 promotes autophagy flux [61].…”

Section: Agingmentioning

confidence: 99%

Electrical stimulation inhibits Val-boroPro-induced pyroptosis in THP-1 macrophages via sirtuin3 activation to promote autophagy and inhibit ROS generation

Liu

Gao

Zheng

et al. 2020

Aging

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INTRODUCTION Atherosclerosis (AS), a chronic inflammatory disease of the arterial wall characterized by gradual build-up of arterial plaque, is the main cause of cardiovascular disease (CVD) [1, 2]. Aging is a major, independent risk factor for AS [3]. Macrophages are main components of the inflammatory plaque and promote AS development across its different stages [4-6]. Accumulating data indicates that pyroptosis, a highly inflammatory form of programmed cell death usually triggered in immune cells upon infection by intracellular pathogens, occurs also in AS-associated macrophages and contributes importantly to plaque instability [7, 8]. Therefore, therapies aimed at inhibiting macrophage pyroptosis may reduce the morbidity and mortality associated with AS.

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Berberine associated photodynamic therapy promotes autophagy and apoptosis via ROS generation in renal carcinoma cells

Cited by 67 publications

References 77 publications

Berberine Improves Chemo-Sensitivity to Cisplatin by Enhancing Cell Apoptosis and Repressing PI3K/AKT/mTOR Signaling Pathway in Gastric Cancer

Berberine Improves Chemo-Sensitivity to Cisplatin by Enhancing Cell Apoptosis and Repressing PI3K/AKT/mTOR Signaling Pathway in Gastric Cancer

Berberine—A potent chemosensitizer and chemoprotector to conventional cancer therapies

Electrical stimulation inhibits Val-boroPro-induced pyroptosis in THP-1 macrophages via sirtuin3 activation to promote autophagy and inhibit ROS generation

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