Berberine and Sanguinarine quantitation in<i>Argemone mexicana</i>L. (Papaveraceae) tissues by TLC-<i>in situ</i>fluorography

Monforte‐González, Miriam; Guízar‐González, Cecilia; Rubio-Piña, Jorge; Carrillo-Pech, Mildred; Vázquez‐Flota, Felipe

doi:10.1556/jpc.25.2012.4.14

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…These values accounted for about 40% of the total alkaloid fraction, gravimetrically estimated in all cases. These alkaloid values were consistent to those previously determined in our laboratory 6 and comparable to other plants, such as Chelidonium and Fumaria.…”

Section: Alkaloid Quantitation In Tissue Extracts From a Mexicanasupporting

confidence: 91%

“…These tissues were selected since they present high alkaloid contents 5,6 and variations along the plant´s life cycle had been observed. 5,6 Tissues were extracted as described under Experimental. The main alkaloids (Bn, C and S) were clearly separated in root extracts from mature plants, where significant amounts of the three alkaloids have been found (Figure 3).…”

Section: Alkaloid Quantitation In Tissue Extracts From a Mexicanamentioning

confidence: 99%

“…Methods for the analysis of BIA's include chromatographic separation using paper or silica plates (TLC), as well as non-polar columns for liquid chromatography (HPLC). [6][7][8] However, some of these require a long time of analysis or have been used mainly for qualitative purposes. [9][10][11][12] In here, we have implemented a simple method for the efficient separation and quantitation of the main alkaloids present in A. mexicana tissues.…”

Section: Introductionmentioning

confidence: 99%

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HPLC-DAD determination of berberine, chelerythrine and sanguinarine in the mexican prickly poppy (Argemone mexicana L. Papaveraceae), a medicinal plant

Xool-Tamayo¹,

Graniel-Sabido²,

Mirón-López³

et al. 2017

Quim. Nova

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A sensitive, simple, rapid and reliable HPLC-DAD method for the analysis of the benzylisoquinoline alkaloids (BIA's) content in Argemone mexicana (Papaveraceae) is presented. This method allows the simultaneous separation and quantitation of berberine (Bn), chelerythrine (C) and sanguinarine (S) in extracts from A. mexicana tissues, reducing time of analysis in comparison to previous reports. Alkaloids were separated on a C18 Hypersil Gold column using an acetonitrile gradient (20 to 70%) in 1% acetic acid in water. Alkaloids were identified based on retention times and UV spectra and quantified at 254 nm. Linearity between 0.5-20 µg mL -1 was observed for Bn, C and S, with limits of detection (LOD) and quantitation (LOQ) of 0.11 and 0.33 for Bn, 0.10 and 0.30 for C and 0.05 and 0.15 for S, respectively. Maximal intra-and inter-day variation values were < 0.49% in all cases, with alkaloids' recoveries higher than 95%. System suitability tests (SST), including resolution (Rs), retention factor (K'), selectivity (a), tailing factor and number of theoretical plates were performed according to the United States Pharmacopeia (USP), fulfilling recommended values. The method proved to be efficient and reproducible when analyzing different tissues of field-collected A. mexicana plants.

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Section: Alkaloid Quantitation In Tissue Extracts From a Mexicanasupporting

confidence: 91%

Section: Alkaloid Quantitation In Tissue Extracts From a Mexicanamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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HPLC-DAD determination of berberine, chelerythrine and sanguinarine in the mexican prickly poppy (Argemone mexicana L. Papaveraceae), a medicinal plant

Xool-Tamayo¹,

Graniel-Sabido²,

Mirón-López³

et al. 2017

Quim. Nova

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“…The samples were freeze-dried and ground to a fine powder using a mechanical mill (IKA Tube Mill CS001; Wilmington NC). The alkaloids were extracted from 50 mg powdered tissues in 10 mL 0.5% hydrochloric acid in methanol, as previously described [ 16 ]. Briefly, the powders were incubated for two hours with gentle shaking at room temperature, and the debris was separated by filtration.…”

Section: Methodsmentioning

confidence: 99%

“…The alkaloids were separated by TLC, using a mixture of n-butanol:water:NH 4 OH (8:1:1), or benzene:ethanol (9:1), for berberine ( R f 0.29) and sanguinarine ( R f 0.53) detection, respectively. After the chromatographic separation, the alkaloids were quantified by in situ fluorescence in a Camag TLC Scanner 4 (Muttenz Switzerland) controlled by the WinCATS 1.4.10 planar chromatography manager [ 16 ]. The analysis was performed in triplicates.…”

Section: Methodsmentioning

confidence: 99%

Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)

Xool-Tamayo

Tamayo-Ordóñez

Monforte‐González

et al. 2021

Plants

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The synthesis of the benzylisoquinoline alkaloids, sanguinarine and berberine, was monitored in Argemone mexicana L. (Papaveracea) throughout the early stages of its hypocotyl and seedling development. Sanguinarine was detected in the cotyledons right after hypocotyl emergence, and it increased continuously until the apical hook unbent, prior to the cotyledonary leaves unfolding, when it abruptly fell. In the cotyledonary leaves, it also remained at low levels. Throughout development, berberine accumulation required the formation of cotyledonary leaves, whereas it was quickly detected in the hypocotyl from the time it emerged. Interestingly, the alkaloids detected in the cotyledons could have been imported from hypocotyls, because no transcriptional activity was detected in there. However, after turning into cotyledonary leaves, important levels of gene expression were noted. Taken together, these results suggest that the patterns of alkaloid tissue distribution are established from very early development, and might require transport systems.

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A validated method for the thin-layer chromatographic in situ autofluorescence densitometric quantitation of the benzylisoquinoline alkaloids berberine and sanguinarine

Estrada-Alfaro

Monforte‐González

Escobar-Chan

et al. 2022

JPC-J Planar Chromat

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Berberine and Sanguinarine quantitation inArgemone mexicanaL. (Papaveraceae) tissues by TLC-in situfluorography

Cited by 10 publications

References 5 publications

HPLC-DAD determination of berberine, chelerythrine and sanguinarine in the mexican prickly poppy (Argemone mexicana L. Papaveraceae), a medicinal plant

HPLC-DAD determination of berberine, chelerythrine and sanguinarine in the mexican prickly poppy (Argemone mexicana L. Papaveraceae), a medicinal plant

Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)

A validated method for the thin-layer chromatographic in situ autofluorescence densitometric quantitation of the benzylisoquinoline alkaloids berberine and sanguinarine

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