Berberine and a Berberis lycium extract inactivate Cdc25A and induce α-tubulin acetylation that correlate with HL-60 cell cycle inhibition and apoptosis

Khan, Musa; Giessrigl, Benedikt; Vonach, Caroline; Madlener, Sibylle; Prinz, Sonja; Herbaceck, Irene; Hölzl, Christine; Bauer, Sabine; Viola, Katharina; Mikulits, Wolfgang; Quereshi, Rizwana Aleem; Knasmüller, Siegfried; Grusch, Michael; Kopp, Brigitte; Krupitza, Georg

doi:10.1016/j.mrfmmm.2009.11.001

Cited by 49 publications

(29 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Berberine was shown to exert anti-proliferative effects on numerous human cancer cells in vitro , including breast cancer, leukemia, melanoma, epidermoid carcinoma, hepatoma, cholangiocarcinoma, colorectal cancer, ovarian cancer, pancreatic cancer, oral carcinoma, tongue carcinoma, nasopharyngeal carcinoma, glioblastoma, prostate carcinoma and gastric carcinoma, uterine leiomyoma, as indicated in [105-126]. In vivo studies using animals models with xenografted human tumors have shown that berberine can suppress prostate cancer, neuroblastoma, and leukemia, and inhibit the growth and development of lung metastases in hepatocellular carcinoma, as reviewed in [111-113, 127]. Down-regulation of cyclin B1 and up-regulation of Wee1 by berberine promotes entry of leukemia cells into the G2/M-phase of the cell cycle [113].…”

Section: Cell Cycle Regulation By Natural Compounds Affecting Cyclinsmentioning

confidence: 99%

Natural Compounds as Modulators of Cell Cycle Arrest: Application for Anticancer Chemotherapies

et al. 2017

View full text Add to dashboard Cite

Natural compounds from various plants, microorganisms and marine species play an important role in the discovery novel components that can be successfully used in numerous biomedical applications, including anticancer therapeutics. Since uncontrolled and rapid cell division is a hallmark of cancer, unraveling the molecular mechanisms underlying mitosis is key to understanding how various natural compounds might function as inhibitors of cell cycle progression. A number of natural compounds that inhibit the cell cycle arrest have proven effective for killing cancer cells in vitro, in vivo and in clinical settings. Significant advances that have been recently made in the understanding of molecular mechanisms underlying the cell cycle regulation using the chemotherapeutic agents is of great importance for improving the efficacy of targeted therapeutics and overcoming resistance to anticancer drugs, especially of natural origin, which inhibit the activities of cyclins and cyclin-dependent kinases, as well as other proteins and enzymes involved in proper regulation of cell cycle leading to controlled cell proliferation.

show abstract

Section: Cell Cycle Regulation By Natural Compounds Affecting Cyclinsmentioning

confidence: 99%

Natural Compounds as Modulators of Cell Cycle Arrest: Application for Anticancer Chemotherapies

et al. 2017

View full text Add to dashboard Cite

show abstract

“…It also caused G1 cell cycle arrest, suppressed invasiveness and decreased MMP-2 activity in melanoma cell lines. Berberis lycium Royle extracts and its alkaloids (berberine and palmatine) were evaluated for antiproliferative activity in p53-deficient HL-60 cells (Khan et al 2010). Berberine and the BuOH extract found to be quite effective in inhibiting growth of HL-60 cells.…”

Section: Phytoconstituents As Cell Cycle Inhibitorsmentioning

confidence: 99%

Phytoconstituents as apoptosis inducing agents: strategy to combat cancer

et al. 2015

View full text Add to dashboard Cite

Advancement in the field of cancer molecular biology has aided researchers to develop various new chemopreventive agents which can target cancer cells exclusively. Cancer chemopreventive agents have proficiency to inhibit, reverse and delay process of carcinogenesis during its early and later course. Chemopreventive agents can act as antioxidative, antimutagenic/antigenotoxic, anti-inflammatory agents or via aiming various molecular targets in a cell to induce cell death. Apoptosis is a kind of cell death which shows various cellular morphological alterations such as cell shrinkage, blebbing of membrane, chromatin condensation, DNA fragmentation, formation of apoptotic bodies etc. Nowadays, apoptosis is being one of the new approaches for the identification and development of novel anticancer therapies. For centuries, plants are known to play part in daily routine from providing food to management of human health. In the last two decades, diverse phytochemicals and various botanical formulations have been characterized as agents that possess potential to execute cancer cells via inducing apoptosis. Data obtained from the research carried out globally pointed out that natural products are the potential candidates which have capability to combat cancer. In the present review, we surveyed literature on natural products which throws light on the mechanism through which these phytochemicals induce apoptosis in cancer cells.

show abstract

“…The anti-tumor action of berberine was extensively reported, in which berberine was shown to modulate several different signal transductions to induce tumor cell cycle redistribution and apoptosis, and to inhibit tumor cell migration [12,13]. Several studies revealed that inhibitory effect of berberine on Cyclin D1 expression in various cancer cell lines including neuroblastoma SK-N-SH & SK-N-MC cells [14], human epidermoid carcinoma A431 cells [15], human prostate carcinoma LNCap, DU145 & PC-3 cells [16], human leukemia cells HL-60 [17], and pulmonary giant cell carcinoma PG cells [11], indicating that Cyclin D1 may be a potential target for berberine in cancer therapy. However, the exact mechanism of Cyclin D1 inhibition in berberine-treated cancer cells has not been well documented.…”

Section: Introductionmentioning

confidence: 99%

Berberine Suppresses Cyclin D1 Expression through Proteasomal Degradation in Human Hepatoma Cells

Wang

Tan

et al. 2016

IJMS

View full text Add to dashboard Cite

The aim of this study is to explore the underlying mechanism on berberine-induced Cyclin D1 degradation in human hepatic carcinoma. We observed that berberine could suppress both in vitro and in vivo expression of Cyclin D1 in hepatoma cells. Berberine exhibits dose- and time-dependent inhibition on Cyclin D1 expression in human hepatoma cell HepG2. Berberine increases the phosphorylation of Cyclin D1 at Thr286 site and potentiates Cyclin D1 nuclear export to cytoplasm for proteasomal degradation. In addition, berberine recruits the Skp, Cullin, F-box containing complex-β-Transducin Repeat Containing Protein (SCFβ-TrCP) complex to facilitate Cyclin D1 ubiquitin-proteasome dependent proteolysis. Knockdown of β-TrCP blocks Cyclin D1 turnover induced by berberine; blocking the protein degradation induced by berberine in HepG2 cells increases tumor cell resistance to berberine. Our results shed light on berberine′s potential as an anti-tumor agent for clinical cancer therapy.

show abstract

Berberine and a Berberis lycium extract inactivate Cdc25A and induce α-tubulin acetylation that correlate with HL-60 cell cycle inhibition and apoptosis

Cited by 49 publications

References 19 publications

Natural Compounds as Modulators of Cell Cycle Arrest: Application for Anticancer Chemotherapies

Natural Compounds as Modulators of Cell Cycle Arrest: Application for Anticancer Chemotherapies

Phytoconstituents as apoptosis inducing agents: strategy to combat cancer

Berberine Suppresses Cyclin D1 Expression through Proteasomal Degradation in Human Hepatoma Cells

Contact Info

Product

Resources

About