Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress

Xiang, Xing-Yang; Liu, Ting; Wu, Yue; Jiang, Xu-shun; He, Jian; Du, Xuqin

doi:10.3892/mmr.2020.11641

Cited by 14 publications

(13 citation statements)

References 52 publications

(52 reference statements)

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“…One of the possible mechanisms of progressive renal injury is podocyte loss, which contributes to the progression of glomerular diseases (Xiong, Meng, & Zhang, 2020). The protective effects of BBR on the palmitic acid (PA)‐induced apoptosis in an in vitro model of podocyte damage have been investigated (Xiang et al, 2021). Intracellular ROS generation and apoptosis were decreased in cells following treatment with BBR (Xiang et al, 2021).…”

Section: Targeting Er Stress By Bbr Under Pathological Conditionsmentioning

confidence: 99%

“…The protective effects of BBR on the palmitic acid (PA)‐induced apoptosis in an in vitro model of podocyte damage have been investigated (Xiang et al, 2021). Intracellular ROS generation and apoptosis were decreased in cells following treatment with BBR (Xiang et al, 2021). Based on the results of this study, BBR reduced the expression of ER stress proteins, including GRP78, PERK, ATF6, ATF4, CHOP, and IRE1 (Xiang et al, 2021).…”

Section: Targeting Er Stress By Bbr Under Pathological Conditionsmentioning

confidence: 99%

“…Intracellular ROS generation and apoptosis were decreased in cells following treatment with BBR (Xiang et al, 2021). Based on the results of this study, BBR reduced the expression of ER stress proteins, including GRP78, PERK, ATF6, ATF4, CHOP, and IRE1 (Xiang et al, 2021). Generally, this study has confirmed the protective effects of BBR against podocyte apoptosis by suppressing ROS production‐mediated ER stress (Xiang et al, 2021).…”

Section: Targeting Er Stress By Bbr Under Pathological Conditionsmentioning

confidence: 99%

“…The protective effects of BBR on the palmitic acid (PA)-induced apoptosis in an in vitro model of podocyte damage have been investigated (Xiang et al, 2021). Intracellular ROS generation and apoptosis were decreased in cells following treatment with BBR (Xiang et al, 2021).…”

Section: Chronic Kidney Diseasementioning

confidence: 99%

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The therapeutic effects of berberine against different diseases: A review on the involvement of the endoplasmic reticulum stress

Yarmohammadi

Hayes

Karimi

2022

Phytotherapy Research

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Various factors interfere with the endoplasmic reticulum (ER) function, which is involved in protein folding and calcium homeostasis. ER dysfunction referred to as ER stress triggers cell death by apoptosis and inflammation. Berberine (BBR) is an alkaloid extracted from the family Berberidacea. It has shown multiple pharmacological activities, including anti-inflammatory, antioxidative, anti-apoptotic, antiproliferative, and antihypertensive. It has been reported that BBR can decrease apoptosis and inflammation following different pathological conditions, which might be mediated by targeting ER stress pathways.In this manuscript, we reviewed the protective potential of BBR against several diseases, such as metabolic disorders, cancer, intestinal diseases, cardiovascular, liver, kidney, and central nervous system diseases, in both in vivo and in vitro studies.

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Section: Targeting Er Stress By Bbr Under Pathological Conditionsmentioning

confidence: 99%

Section: Targeting Er Stress By Bbr Under Pathological Conditionsmentioning

confidence: 99%

Section: Targeting Er Stress By Bbr Under Pathological Conditionsmentioning

confidence: 99%

Section: Chronic Kidney Diseasementioning

confidence: 99%

See 2 more Smart Citations

The therapeutic effects of berberine against different diseases: A review on the involvement of the endoplasmic reticulum stress

Yarmohammadi

Hayes

Karimi

2022

Phytotherapy Research

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show abstract

“…It is closely related to cell replication, proliferation, immunity and cancer. Cell dysfunction and apoptosis are also key factors in the progress of chronic kidney disease (CKD) ( Xiang et al, 2021 ). Excessive apoptosis may lead to disease.…”

Section: Introductionmentioning

confidence: 99%

The expression of apoptosis related genes in HK-2 cells overexpressing PPM1K was determined by RNA-seq analysis

et al. 2022

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Chronic kidney disease (CKD) is a serious disease that endangers human health. It is reported that inhibiting renal cell apoptosis can delay the progress of CKD. Our previous study found that the mice with protein phosphatase Mg2+/Mn2+ dependent 1K (PPM1K) gene deletion had obvious symptoms of glomerular vascular and interstitial vascular dilatation, congestion and hemorrhage, glomerular hemorrhage and necrosis, interstitial fibrous tissue proliferation, decreased urinary creatinine clearance, and increased urinary protein level. In addition, studies have found that PPM1K is essential for cell survival, apoptosis and metabolism. However, no study has confirmed that PPM1K can inhibit renal cell apoptosis. In this study, PPM1K was overexpressed in human kidney-2 cells (HK-2), and the biological process of differentially expressed genes and its effect on apoptosis were comprehensively screened by RNA sequencing (RNA-seq). Through sequencing analysis, we found that there were 796 differentially expressed genes in human renal tubular epithelial cells transfected with PPM1K gene, of which 553 were down-regulated and 243 were up-regulated. Enrichment analysis found that differentially expressed genes may play an important role in amino acid metabolism and biosynthesis. In the GO analysis functional pathway list, we also found that multiple genes can be enriched in apoptosis related pathways, such as G0S2, GADD45A, TRIB3, VEGFA, NUPR1 and other up-regulated genes, and IL-6, MAGED1, CCL2, TP53INP1 and other down-regulated genes. Then we verified these differentially expressed genes by RT-PCR, and found that only the RT-PCR results of G0S2, VEGFA and NUPR1 were consistent with the transcriptome sequencing results. We believe that G0S2, VEGFA, NUPR1 and other genes may participate in the apoptosis process of HK-2 cells induced by PPM1K.In conclusion, these findings provide some data support for the study of HK-2 cell apoptosis mechanism, and also provide a scientific theoretical basis for further study of the effect of PPM1K on kidney disease.

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Berberine promotes the viability of random skin flaps via the PI3K/Akt/eNOS signaling pathway

Chen

Jiang

Liu

et al. 2022

Phytotherapy Research

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Random skin flaps are often used in reconstruction operations. However, flap necrosis is still a common postoperative complication. Here, we investigated whether berberine (C20H19NO5, BBR), a drug with antioxidant activity, improves the survival rate of random flaps. Fifty‐four rats were divided into three groups: control, BBR and BBR + L‐NAME groups (L‐NAME, L‐NG‐Nitro‐arginine methyl ester). The survival condition and the percentage of survival area of the flaps were evaluated on the seventh day after surgery. After animals were sacrificed, angiogenesis, apoptosis, oxidative stress and inflammation levels were assessed by histological and protein analyses. Our findings suggest that berberine promotes flap survival. The level of angiogenesis increased; the levels of oxidative stress, inflammation and apoptosis decreased; the levels of phosphoinositide 3‐kinase (PI3K), phospho‐Akt (p‐Akt) and phospho‐endothelial nitric oxide synthase (p‐eNOS) increased in the flap tissue; and L‐NAME reversed the effects of berberine on random skin flaps. Statistical analysis showed that the BBR group results differed significantly from those of the control and the BBR + L‐NAME groups (p < .05). Our results confirm that berberine is an effective drug for significantly improving the survival rate of random skin flaps by promoting angiogenesis, inhibiting inflammation, attenuating oxidative stress, and reducing apoptosis through the PI3K/Akt/eNOS signaling pathway.

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Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress

Cited by 14 publications

References 52 publications

The therapeutic effects of berberine against different diseases: A review on the involvement of the endoplasmic reticulum stress

The therapeutic effects of berberine against different diseases: A review on the involvement of the endoplasmic reticulum stress

The expression of apoptosis related genes in HK-2 cells overexpressing PPM1K was determined by RNA-seq analysis

Berberine promotes the viability of random skin flaps via the PI3K/Akt/eNOS signaling pathway

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