Berbamine inhibited the growth of prostate cancer cells in vivo and in vitro via triggering intrinsic pathway of apoptosis

Zhao, Yanmin; Lv, Jiaju; Chen, J; Jin, Xunbo; Wang, M W; Su, Zhenhui; Wang, L Y; Zhang, H Y

doi:10.1038/pcan.2016.29

Cited by 33 publications

(27 citation statements)

References 29 publications

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“…This work is the first report focused on exploring the antiproliferative, cytotoxic and apoptosis-inducing effects of bersavine. In previous studies, the structurally related alkaloid berbamine was clearly confirmed as a promising anticancer drug candidate, which was supported by experimental evidence of strong antiproliferative, cytotoxic and proapoptotic activity [6][7][8]12]. Moreover, berbamine is well-known in Chinese medicine and it has been used as a treatment option for decades [11].…”

Section: Discussionmentioning

confidence: 78%

“…Berbamine also exerts anti-inflammatory effects by inhibiting nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinase (MAPK) signalling pathways [7]. Its ability to inhibit proliferation in cancer cells was described repetitively in many experimental studies [8][9][10][11][12][13]. Zhang and coworkers observed the apoptosis-inducing effect of berbamine in colorectal cancer cells by activating p53-dependent apoptotic signalling pathways [14].…”

Section: Introductionmentioning

confidence: 99%

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Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

et al. 2020

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Bersavine is the new bisbenzylisoquinoline alkaloid isolated from the Berberis vulgaris L. (Berberidaceae) plant. The results of cytotoxicity screening 48 h post-treatment showed that bersavine considerably inhibits the proliferation and viability of leukemic (Jurkat, MOLT-4), colon (HT-29), cervix (HeLa) and breast (MCF-7) cancer cells with IC50 values ranging from 8.1 to 11 µM. The viability and proliferation of leukemic Jurkat and MOLT-4 cells were decreased after bersavine treatment in a time- and dose-dependent manner. Bersavine manifested concentration-dependent antiproliferative activity in human lung, breast, ovarian and hepatocellular carcinoma cell lines using a xCELLigence assay. Significantly higher percentages of MOLT-4 cells exposed to bersavine at 20 µM for 24 h were arrested in the G1 phase of the cell cycle using the flow cytometry method. The higher percentage of apoptotic cells was measured after 24 h of bersavine treatment. The upregulation of p53 phosphorylated on Ser392 was detected during the progression of MOLT-4 cell apoptosis. Mechanistically, bersavine-induced apoptosis is an effect of increased activity of caspases, while reduced proliferation seems dependent on increased Chk1 Ser345 phosphorylation and decreased Rb Ser807/811 phosphorylation in human leukemic cells.

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

et al. 2020

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“…Du [24] found that BBM induced cell apoptosis and inhibited the cell proliferation through the PI3K/Akt pathway in lymphoma, the expressions of PI3K and p-Akt/Akt were down-regulated. Zhao [8] proved that BBM inhibited the activity of prostate cancer cells and could destroy the mitochondria of cells. All these researches indicated that the potential of BBM for cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported to possess the effects of anti-oxidation, immunoregulation, and anti-arrhythmia in recent studies [4][5][6][7]. BBM was also revealed to have potential function of antitumor [8][9][10]. However, there were few studies on the anti-tumor effect of BBM in lung cancer,and the in vitro studies just evaluated the cell activity and migration, and the mechanism involved was just Bcl-2/Bax.…”

Section: Introductionmentioning

confidence: 99%

Berbamine Inhibits Cell Proliferation, Migration, and Invasion and Induces Cell Apoptosis of A549 Cells Via Regulating C-Maf, PI3K/Akt and MDM2-P53 Pathways

Liu

Zhang²,

et al. 2020

Preprint

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Background To investigate the influences of berbamine (BBM) on the cell viability, proliferation, and migration of A549 cells in vitro and in vivo, and explore the possible mechanisms.MethodsAfter the A549 cells were treated with BBM, the cell viability and proliferation of the cancer cells were detected by MTT assay, EdU assay, and colony formation assay. Migration and invasion of cancer cells were illustrated through wound scratch assay and transwell assay. Apoptosis of cancer cells was evaluated by trypan blue dye exclusion assay and elisa assay. Beside, the expression of PI3K/Akt signal pathway-related proteins and c-Maf were detected employing western blotting assay. Xenografted model of NSCLC was used to detect the effect of BBM on tumor growth and metastasis in vivo.ResultsMTT assay showed that BBM inhibited the viability of A549 cells in a concentration-dependent manner and time-dependent manner. The results from the colony formation assay and EdU assay revealed that BBM (10 µM) could significantly inhibit the proliferation of A549 cells (P＜0.001). And BBM (10 µM) significantly inhibited the migration and invasion in the wound scratch assay and transwell assay (P＜0.05). Trypan blue assay and elisa assay indicated that BBM (20 µM) significantly induced apoptosis of A549 cells. The nude mice assay manifested the tumor volume was significantly shrank by BBM (20 mg/kg) (P＜0.05). Western blotting assay showed that the PI3K/Akt and MDM2-p53 signaling pathways were inhibited by BBM, and the expression of c-Maf was downregulated by BBM. ConclusionsBBM could inhibit the proliferation and metastasis, and induce apoptosis of A549 cells in vitro and in vivo, these effects may be achieved by reducing the expression of c-Maf and regulating the PI3K/Akt and MDM2-p53 pathways.

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“…5. Berbamine inhibited the growth of prostate cancer cells in vivo and in vitro via triggering intrinsic pathway of apoptosis [5]. Researchers discovered that Berbamine could inhibit prostate cancer progression via activating intrinsic apoptosis pathway, which offered an opportunity to develop new managing strategy for prostate cancer.…”

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confidence: 99%

Prostate cancer and prostatic diseases Best of China, 2018

Zhu

2018

Prostate Cancer Prostatic Dis

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Berbamine inhibited the growth of prostate cancer cells in vivo and in vitro via triggering intrinsic pathway of apoptosis

Abstract: BBM exhibited strong anti-PCa activities in vitro and in vivo relying on activating caspase-3 via intrinsic pathway of apoptosis, holding a great promise to develop new strategies for PCa.

Cited by 33 publications

References 29 publications

Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

Berbamine Inhibits Cell Proliferation, Migration, and Invasion and Induces Cell Apoptosis of A549 Cells Via Regulating C-Maf, PI3K/Akt and MDM2-P53 Pathways

Prostate cancer and prostatic diseases Best of China, 2018

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