�ber die Mortonsche Metatarsalgie

Macháček, Josef; Machacek, Felix

doi:10.1007/bf00418811

Cited by 7 publications

(11 citation statements)

References 10 publications

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“…Systemic conditions that can lead to secondary metatarsalgia include trauma, 28 inflammatory arthropathy, gout, 6,20 arthritides and instability of the MTP joints, 13,50 Morton's neuroma, 11,36 tarsal tunnel syndrome 48 and Freiberg's infraction. 13 Not all of these conditions directly affect the metatarsal, and instead indirectly overload the forefoot.…”

Section: Secondary Metatarsalgiamentioning

confidence: 99%

Current Concept Review: Metatarsalgia

Espinosa

Maceira²,

Myerson³

2008

Foot Ankle Int.

100

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Section: Secondary Metatarsalgiamentioning

confidence: 99%

Current Concept Review: Metatarsalgia

Espinosa

Maceira²,

Myerson³

2008

Foot Ankle Int.

100

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“…Consistent with earlier studies, this Cdc42 biosensor showed Cdc42 activity localized at active regions of the cell edges and in protrusions (Figure 5b). 6,11,15 Uniform background fluorescence from unattached dye could be greatly reduced by washing the cells in fresh media. Use of the mCBD-TAG-EGFP control construct reduced the level of observed Cdc42 activation and failed to show the localization observed with CBD.…”

Section: Resultsmentioning

confidence: 99%

“…This is frequently accomplished using biosensors based on fluorescence resonance energy transfer (FRET) between fluorescent proteins; many such biosensors are fully genetically encoded so can be readily expressed in cells. [1][2][3][4][5][6][7][8] Another class of biosensor is based on covalently attaching environment-sensitive fluorescent dyes to proteins at positions where their fluorescence responds to conformational changes or protein-protein interactions. This approach can be more sensitive than FRET because the fluorophore is excited directly (rather than indirectly by the FRET donor) and because one can use dyes that are brighter than fluorescent proteins.…”

Section: Introductionmentioning

confidence: 99%

“…30 We based our dye development on extensively characterized merocyanine fluorophores that have a useful combination of brightness and environment sensitivity and with extended conjugation for excitation above 550 nm (to avoid cellular autofluorescence). 6, We sought to confer membrane permeability on these proven dyes, to enable biosensor production within cells, and for generating membrane-permeable "small molecule biosensors. "…”

Section: Introductionmentioning

confidence: 99%

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Membrane-Permeant, Environment-Sensitive Dyes Generate Biosensors within Living Cells

et al. 2019

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Dyes with environment-sensitive fluorescence have proven useful to study the spatio-temporal dynamics of protein activity in living cells. When attached to proteins, their fluorescence can reflect protein conformational changes, posttranslational modifications or protein interactions. However, the utility of such dye-protein conjugates has been limited because it is difficult to load them into cells. They usually must be introduced using techniques that perturb cell physiology, limit throughput, or generate fluorescent vesicles (e.g. electroporation, microinjection, or membrane transduction peptides). Here we circumvent these problems by modifying a proven, environment-sensitive biosensor fluorophore so that it can pass through cell membranes without staining intracellular compartments, and can be attached to proteins within living cells using unnatural amino acid (UAA) mutagenesis. Reactive groups were incorporated for attachment to UAAs or small molecules (mero166, azide; mero167, alkyne; mero76, carboxylic acid). These dyes are bright and fluoresce at long wavelengths (reaching ε = 100,000 M −1 cm −1 , φ = 0.24, with excitation 565 nm and emission 594 nm). The utility of mero166 was demonstrated by in-cell labeling of an UAA to generate a biosensor for the small GTPase Cdc42. In addition, conjugation of mero166 to a small molecule produced a membrane-permeable probe that reported the localization of the DNA methyltransferase G9a in cells. This approach provides a strategy to access biosensors for many targets, and to more practically harness the varied environmental sensitivities of synthetic dyes.

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“…It was used for the determination of the strength values fluctuation related to the change of the environment temperature of single sets (Macháček & Majer 1981).…”

Section: Methodsmentioning

confidence: 99%

Technological and constructional aspects affecting bonded joints

Müller¹,

Chotěborský²,

Krmela³

2007

Res. Agric. Eng.

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Abstract:Adhesive bonding is one of many materials connecting methods. In the last ten years periods the bonding technology noted a boom almost in all industrial branches. The use of bonding technology in the engineering and repairing industry brings considerable savings. Saving in costs, in critical metallic materials and in time are reached and the decrease of the joint weight, too. Therefore the bonding technology pertains to the modern jointing methods even though it is a very old technique. The adhesive bonding technology is influenced by a number of factors which affect the adhesive bond strength. Correcting coefficients have to be considered in construction calculations too. The correcting coefficients correct the strength deviations caused by particular factors. In the paper there are published laboratory experiments results.

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�ber die Mortonsche Metatarsalgie

Cited by 7 publications

References 10 publications

Current Concept Review: Metatarsalgia

Current Concept Review: Metatarsalgia

Membrane-Permeant, Environment-Sensitive Dyes Generate Biosensors within Living Cells

Technological and constructional aspects affecting bonded joints

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