�ber die gaschromatographische Blutalkoholbestimmung. Analyse der Dampfphase

Machata, G

doi:10.1007/bf01218048

Cited by 64 publications

(19 citation statements)

References 12 publications

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“…The volatile substances concerned were analyzed by GC-MS using the head space method [1,3,7], as follows: the formalinfixed organ specimens were washed with redistilled water and excess water was removed. A slice weighing approximately 0.2-0.5 g was placed in a vial and an aliquot (0.5 ml) of the internal standard (IS) solution, 0.5 mg/ml ethanol-d6 (d5) and 20 gg/ml toluene-d8 in 10% (v/v) aqueous Tween 20 solution, was added.…”

Section: Methodsmentioning

confidence: 99%

Toxicological analysis of drugs and poisons in formalin-fixed organ tissues

et al. 1994

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Diethylether, chloroform and toluene were administered by inhalation and ethanol intravenously to rabbits. As soon as possible after death, tissue specimens were collected from the brain, lung, liver, kidney and skeletal muscle and fixed in non-buffered 10% formalin at room temperature (10-20 degrees C) for 4 different periods (1, 2, 5 and 14 days). The volatile substances were analyzed and identified by gas chromatography/mass spectrometry (GC-MS). The measured concentrations of ethanol, diethylether, chloroform and toluene in the brain tissue 1 day after fixation decreased to 8, 23, 73 and 84% respectively compared with those in the non-fixed brain tissue (100%). The rank order of the rate of decrease in the fixed state was: ethanol > diethylether >> chloroform > toluene. These volatile substances could be detected clearly in all the tissue specimens, even after a 14-day fixation period. These results provide useful toxicological information that will help to differentiate whether volatile substances have been administered antemortem or postmortem.

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Section: Methodsmentioning

confidence: 99%

Toxicological analysis of drugs and poisons in formalin-fixed organ tissues

et al. 1994

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“…Concentrations of alcohol in blood and alcohol reference solutions were measured by automated gas chromatographic headspace analysis with a ~I t i f r a c t F40 instrument (Perkin-Elmer Corp., Norwalk, Conn. 06852) according to the method of MACHATA (35) modified by adding I g of sodium chloride to I ml of blood or other liquid and equilibrating at 65°C for 45 minutes prior to headspace analysis.…”

Section: Apparatus Methods and Proceduresmentioning

confidence: 99%

Studies in breath-alcohol analysis: Biological factors

Dubowski

1975

Z Rechtsmed

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Various biological factors affecting breath-alcohol analysis were studied experimentally. End-expiratory temperatures in 55 healthy subjects were found to range from 32.41 to 35.69 degrees C with a mean of 34.53 degrees C. Forced vital capacity in the same subjects ranged from 1825 to 6550 ml with a mean of 4038 ml, and maximum exhalation after normal inhalation ranged from 1180 to 4550 ml with a mean of 2730 ml. It was found that 65-70% of available breath must be discarded before the alveolar plateau is reached during expiration. End-expiratory (alveolar) carbon dioxide in 155 healthy subjects was 3.5-8.3% by volume (mean = 6.52). After oral alcohol intake, retained mouth-alcohol in 8 subjects had disappeared after 11 minutes without subsequent water-rinsing of the mouth, and after 8 minutes with rinsing. Water condensation in plastic mouthpieces/saliva traps during breath sampling yielded mean weight gains of 13.0, 8.6, and 4.6 mg., respectively, at initial mouthpiece temperatures of 3 degrees C, 22.5 degrees C, and 34.7 degrees C, respectively.

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“…For ethanol measurements two consecutive samples were combined. Ethanol concentrations were determined by gas chromatography [14].…”

Section: Methodsmentioning

confidence: 99%