�ber den Mangel an Pl�ttchen-Antifibrinolysin beim v. Willebrand-J�rgens-Syndrom

Cell walls of Microcystis sp. PCC 7806 were purified from cell homogenates by sucrose density centrifugation and Triton X-100 extraction. The outer membrane contained carotenoids, two major peptidoglycan-associated proteins (Mr 49,000 and 52,000), and lipopolysaccharide (LPS) as indicated by the presence of 3-hydroxy fatty acids (3-OH-14:0, 3-OH-16:0, 3-OH-18:0), 4-oxo-18:0 fatty acid, and GlcN as lipid A components in addition to rare O-methyl sugars (2-O-methyl-6-deoxyhexoses I and II). The peptidoglycan (A1 gamma-type) was found to be covalently linked to a wall polysaccharide composed of GlcN, ManN, Man, Glc, and phosphate.

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Cell Walls Peptidoglycan-polysaccharide Compmentioning

confidence: 99%

Cell wall constituents of Microcystis sp. PCC 7806

Jürgens

Martin

Weckesser

1989

“…The finding of LPS in the outer membrane of Prochlorothrix hollandica together with the presence of a peptidoglycan-polysaccharide complex within the cell wall [26] similar in chemical composition as found in cell walls of various cyanobacteria [30,40] are striking features. Thus, a close phylogenetical relationship between Prochlorothrix hollandica and the cyanobacteria is suggested.…”

Section: Discussionmentioning

confidence: 99%

“…Amino acids, amino sugars, fatty acids, neutral sugars, and phosphate were determined as described previously [30]. Estimation of dOclA was performed by using a colorimetric assay [31].…”

Section: Chemical Analysesmentioning

confidence: 99%

Lipopolysaccharide in the outer membrane of the filamentous prochlorophyte Prochlorothrix hollandica

Jürgens¹

1989

A lipopolysaccharide (LPS) fraction was isolated from Prochlorothrix hollandica by hot phenol/water extraction. Negatively stained preparations of an aqueous LPS dispersion showed the triple-layered appearance of the LPS aggregates. Glucose (main sugar), rhamnose, fucose, galactose, mannose, xylose, and 3-O-methyl-xylose were found as the constituents of the polysaccharide moiety. Glucosamine and the 3-hydroxy fatty acids, 3-OH-16:0, 3-OH-14:0, and the rarely detected iso-3-OH-15:0, constitute the lipid A of the LPS. L-glycero-D-manno-heptose and 3-deoxy-D-manno-2-octulosonic acid (dOclA), typical components of inner core oligosaccharides from enterobacterial LPS, were lacking in the isolated LPS fraction from Prochlorothrix hollandica.

“…Other characteristics were similar to the cyanobacteria with respect to the fine structure of the cell wall, peptidoglycan structure, lipid and carotenoid types, genome size, and partial oligonucleotide sequence homologies of 16S rRNA (for a review see [8]). Prochlorothrix hollandica and Prochloron didemni shared commGn caaracteristics such as the cell wall fine structure [1,9], peptidoglycan type [10], thylakoid and cytoplasmic membrane organization [9,11], and photosynthetic antenna systems [12,13]. A lipopolysaccharide similar in chemical composition to that of the cyanobacteria [14] was found in the outer membrane of Prochlorothrix hollandica [15].…”

Section: Introductionmentioning

confidence: 99%

Major outer membrane proteins of Prochlorothrix hollandica (Prochloraceae)

Jürgens

1990

1. Summary Triton‐insoluble cell walls of the prochlorophyte Prochlorothrix hollandica were isolated by using differential centrifugation of cell homogenates followed by Triton X‐100 extraction of the cell envelopes. Polypeptide patterns of solated cell walls were dominated by two major outer membrane proteins with molecular masses of Mr 56 000 and 59 000, respectively. Differential sodium dodecyl sulfate (SDS)‐extractions of the cell envelopes yielded SDS‐insoluble peptidogly‐can‐protein complexes which contained both major ofter membrane proteins highly enriched and non‐covalently associated with the peptidoglycan layer of Prochlorothrix hollandica.