�ber den Kohlenhydratumsatz in Chlorella unter dem Einflu� von blauem und rotem Licht

Laudenbach, Beatrice T.; Pirson, André

doi:10.1007/bf00411258

Cited by 19 publications

(2 citation statements)

References 19 publications

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“…an enhancement in the activities of several enzymes Miyachi 1975, Kowallik andRuyters 1976), of increased intracellular accumulation of ammonia from protein degradation (Miyachi and Miyachi 1985), of acidification of the medium (Kamiya 1996), of additional uptake of nitrate (Kamiya 1997). Most of the effects were also found with wild-type cells of Chlorella (Pickett and French 1967, Laudenbach and Pirson 1969, Ruyters 1987.…”

Section: Introductionmentioning

confidence: 98%

Blue‐light‐control of the uptake of amino acids and of ammonia in Chlorella mutants

Kamiya

Saitoh

2002

Physiologia Plantarum

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In non-photosynthetic, yellow or colourless mutant cells of Chlorella kessleri, grown with nitrate as sole nitrogen source, blue light inhibited the uptake of the amino acids glycine, proline and arginine and of ammonia in growing cells, while it enhanced the uptake of these amino acids in resting cells. On the other hand, in cells grown with ammonia as the only nitrogen source without nitrate reductase activity, blue light did not influence the uptake of amino acids and of ammonia in growing cells, while it enhanced the uptake of amino acids in resting cells. Addition of methionine sulphoximine, a potent inhibitor of glutamine synthetase, to growing cells, resulted in intracellular ammonia-accumulation and inhibition of uptake of glycine and of ammonia. For the colourless mutant, blue light was shown to activate purified nitrate reductase. These results indicate that in the mutant cells of Chlorella examined, uptake of ammonia seems to be influenced by nitrate reductase and the uptake of amino acids was influenced by both nitrate reductase and an unknown blue-light-receptor(s). The uptake of urea in mutant cells is not influenced by the irradiation with blue light. Uptake of glycine was also increased after addition of glucose (hexose) in the dark. Because blue light is known to enhance the breakdown of starch, a reaction producing glucose for oxidative degradation in the algae used, the role of glucose (hexose) in the blue light-affected uptake of amino acids is discussed.

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Section: Introductionmentioning

confidence: 98%

Blue‐light‐control of the uptake of amino acids and of ammonia in Chlorella mutants

Kamiya

Saitoh

2002

Physiologia Plantarum

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“…Different levels of glucose being responsible for blue light-dependent changes in the activity of pyruvate kinase in a chlorophyll-free Chlorella mutant have been discussed earlier (Kowallik and Ruyters 1976). The effect of specific wavelengths of light on PFK activity in C kessleri would then take place via regulation of the distribution of carbohydrate within the cell; Laudenbach and Pirson (1969) have proposed a decreasing influence of red light on the permeability of the chloroplast envelope, resulting in storage of starch in this organelle. Electron micrographs by Wilhelm et al (1985) support this idea.…”

Section: Resultsmentioning

confidence: 99%

Influence of blue light on the activity of phosphofructokinase in Chlorella kessleri

Kowallik

Grotjohann

1989

Physiologia Plantarum

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Grotjohann, N. and Kowallik, W. 1989. Influence of blue light on the activity of phosphofructokinase in Chlorella kessleri. -Physiol. Plant. 73: 43^6.In crude extracts of Chlorella kessleri Fott and Novakova cells grown autotrophically in white light the activity of phosphofructokinase (PFK, EC 2.7.1.11) is 62.9 ± 1.3 nmol (mg protein) ' min ' under optimized test conditions. It is greatly increased in red [88.3 ± 1.8 nmol (mg protein) ' min^'], but somewhat decreased [37.0 ± 0.3 nmol (mg protein) ' min ') in blue light of equal productivity. Mixtures of blue and red light yield the low activity as long as blue light represents at least 33% of the total quantum fluence rate. The rough wavelength dependence of the counteracting effect of short wavelength light on the increasing effect of red light exhibits a broad peak at 460 nm, reminiscent of action spectra of the blue/UV photoreceptor(s). Upon transfer of red light-grown cells to blue light, the decrease develops slowly within 72 h; it cannot be prevented by 3-(3,4-dichlorophenyl)-l,l-dimethyl urea (DCMU). Since there is less carbohydrate in blue than in red light-exposed cells, correlations between biosynthesis of PFK and level of carbohydrate are discussed, based on the assumption that red light decreases and/or blue light increases the transport of metabolites across the chloroplast envelope.

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Kandeler¹

1972

Fortschritte Der Botanik

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�ber den Kohlenhydratumsatz in Chlorella unter dem Einflu� von blauem und rotem Licht

Cited by 19 publications

References 19 publications

Blue‐light‐control of the uptake of amino acids and of ammonia in Chlorella mutants

Blue‐light‐control of the uptake of amino acids and of ammonia in Chlorella mutants

Influence of blue light on the activity of phosphofructokinase in Chlorella kessleri

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