�ber den Einflu� toxischer Substanzen auf die Biolumineszenz von Bakterien

K�ssler, Franz; Frucht, A. -H.; M�ller, Gunther

doi:10.1007/bf00592033

Cited by 4 publications

(2 citation statements)

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“…The bacterial bioluminescence assay utilising Vibrio fischeri, or other natural luminescent organisms (Kössler et al, 1965) has been used for more than 35 years in bioassays to assess acute toxic effects of substances in the aquatic environment. However, established acute (e.g., Microtox, AZUR, 1997) and chronic (Zieniss and Grabert, 1995) bioassays, proprietary or otherwise, using the natural light-emitting V. fischeri have had limited use in protecting the biological stages of waste water treatment works (WWTW) from toxic influents.…”

Section: Introductionmentioning

confidence: 99%

“…Toxic substances causing a disturbance to the normal metabolism of the bacterium, result in a reduction or cessation of light output. The degree of toxicity is proportional to the measured light loss (Kössler et al, 1965) and there is generally a good correlation between toxicity determined using V. fischeri and that measured by bioassays using higher organisms such as Daphnia, Fathead minnow, and Rainbow trout (Gaelli et al, 1994;Kaiser, 1998;Kaiser and Palabrica, 1991;Toussaint et al, 1995;Schramm et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Testing the toxicity of influents to activated sludge plants with the Vibrio fischeri bioassay utilising a sludge matrix

Hoffmann

Christofi

2001

Environmental Toxicology

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To protect the bioceonosis within activated sludge, a method of predicting the toxic effect of influents to the biological treatment stage of waste water treatment plants, based on DIN method 38412 L 34, has been developed. A population of the luminescent marine bacterium Vibrio fischeri was incorporated into a sludge testing matrix derived from a model laboratory and real activated sludge plants. The sludge was challenged with different concentrations of pure toxicants and complex aqueous samples, and light output by V. fischeri monitored. The results were compared to toxicant testing in the absence of sludge (standard test). The modified method was found to be less sensitive for some toxicants tested than the standard DIN and other bioluminescent tests, but considered more realistic as it provides buffering and takes into account sorption which can affect the sensitivity of the test towards some compounds. The method is comparable in terms of ease of use, speed, reproducibility and cost effectiveness to standard V. fischeri luminescence methods.

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Section: Introductionmentioning

confidence: 99%