�ber das aortenendothel

Sinapius, D.

doi:10.1007/bf00957503

Cited by 50 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the presence of these atypical endothelial cells is unlikely to be solely responsible for the endothelial dysfunction since, they represent a modest percentage (less than 15%) of the total cell population. The morphology of these cultured giant regenerated endothelial cells resembles closely that described for the giant multinucleated cells, observed in vitro and in vivo , with endothelium denudation in the rabbit (Poole et al , 1958), haemodynamic stress in the rabbit (Fallon & Stehbens, 1972), atherosclerosis in rabbits and in man (Efskind, 1941; Sinapius, 1952; Tokunaga et al , 1989; Tashiro et al , 1994), and with senescence in bovines and man (Tokunaga et al , 1989; Augustin‐Voss et al , 1993). The appearance of these giant cells may indicate a change in endothelial cell phenotype involved in the phenomena of endothelial alteration.…”

Section: Discussionsupporting

confidence: 72%

Morphological heterogeneity with normal expression but altered function of G proteins in porcine cultured regenerated coronary endothelial cells

Borg-Capra

Fournet-Bourguignon

Janiak

et al. 1997

British J Pharmacology

View full text Add to dashboard Cite

1 Experiments were designed to investigate whether the pertussis toxin-dependent endothelial dysfunction following balloon injury is due to a reduced expression or an insu cient function of Gproteins. 2 Endothelium-dependent responses of porcine coronary arteries were examined in vitro by use of conventional organ chambers. Morphological analysis was performed by isolating and culturing the endothelial cells from these arteries. The expression of Gi-proteins in regenerated endothelial cells was measured by Western blots and immunolabelling. The function of G-proteins was assessed by measuring the GTPase activity of cultured endothelial cells. 3 Eight days following denudation, endothelial regrowth was con®rmed by histological examination and by demonstrating the presence of endothelium-dependent relaxations to bradykinin and 5-hydroxytryptamine (5-HT). In primary culture, the regenerated endothelial cells displayed a`cobblestone' pattern as seen with native endothelial cells. 4 Twenty eight days after denudation, the endothelium-dependent relaxations induced by 5-HT were impaired, but those to bradykinin were maintained. However, the latter were reduced when endotheliumdependent hyperpolarization was prevented. 5 Twenty eight days after denudation, multinucleated giant cells were present in the regenerated but not in the native cultured endothelial cell populations. These regenerated endothelial cells incorporated less tritiated thymidine than native endothelial cells. 6 The intensities of the bands on the immunoblot of the regenerated endothelial cells, when several antibodies against Gia1/a2/a3 were used, were the same as those obtained in native endothelial cells. The immunolabelling with the same antibodies was similar between the giant cells and the regenerated endothelial cells of normal size. The hydrolysis of GTP was lower in regenerated than in native endothelial cell membranes. 7 In conclusion, endothelium-dependent relaxations mediated by Gi-proteins are impaired in balloon denuded coronary arteries. This dysfunction following regeneration cannot be explained by a reduced expression of Gi proteins but rather re¯ects an abnormal function of the G-proteins in the regenerated endothelium.

show abstract

Section: Discussionsupporting

confidence: 72%

Morphological heterogeneity with normal expression but altered function of G proteins in porcine cultured regenerated coronary endothelial cells

Borg-Capra

Fournet-Bourguignon

Janiak

et al. 1997

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…• Bizarre multinucleated cells, so prevalent in the endothelium of atherosclerotic aortas (1)(2)(3), are also found during endothelial repair and regeneration (1,(4)(5)(6). Recent isotope labeling studies in the aortas of experimental animals have revealed that the endothelial lining of blood vessels is renewed continuously, and an elevated endothelial mitotic index has been observed around the orifices of branches (7)(8)(9), which are well-recognized sites of predilection for atherosclerosis in man.…”

mentioning

confidence: 99%

Venous Endothelium of Experimental Arteriovenous Fistulas in Rabbits

Fallon

Stehbens

1972

Circulation Research

View full text Add to dashboard Cite

Since hemodynamic factors have been implicated in the localization and pathogenesis of atherosclerosis, the effect of hemodynamic stress on endothelium was investigated. The endothelium of the inferior vena cava of 24 rabbits with aortocaval fistulas was stained in situ and examined by the Hautchen technique at postoperative intervals ranging from 1 to 50 weeks. Cell counts revealed that, in the anastomosed vein of the fistula, an initially high frequency of abnormal cells decreased 3-12 weeks postoperatively but rose again within 20-50 weeks to levels significantly higher than those in either normal venous endothelium or sham-operated veins. An originally high mitotic index at 1-2 weeks decreased with time but still remained significantly larger than that observed in sham-operated rabbits. In most rabbits with arteriovenous shunts, a small area of the inferior vena cava near the fistula was devoid of endothelium, covered with a thin deposit of mural thrombi, and considered to be a jet lesion. The findings demonstrate that hemodynamic factors can cause endothelial injury, increased turnover of endothelial cells, and formation of multinucleated endothelial cells characteristic of regenerating endothelium.

show abstract

“…One view is that new endothelium develops from pre-existing endothelium, with disagreement as to the type of proliferative activity, at least in normal blood vessels. Although Zahn (1) observed mitotic activity in the endothelium, Efskind (2), Altschul (3), and Sinapius (4) reported that endothelium replaces itself almost exclusively by amitosis under physiological conditions. In injured vessels endothelial mitosis has been demonstrated (5)(6)(7).…”

mentioning

confidence: 99%

Myointimal Cells as a Possible Source of Replacement for Endothelial Cells in the Rabbit

Ts’ao

1968

Circulation Research

View full text Add to dashboard Cite

Regeneration of damaged endothelium has been studied by means of mechanical injury to rabbit abdominal aorta and inferior vena cava. Ultrastructural evidence suggests that myointimal cells can become endothelial cells. It is postulated that such a myogenic replacement of endothelial cells may occur under normal circumstances in the aorta. Injury to blood vessels merely accelerates this process.ADDITIONAL KEY WORDS blood vessel repair myointimal cell

show abstract

�ber das aortenendothel

Cited by 50 publications

References 15 publications

Morphological heterogeneity with normal expression but altered function of G proteins in porcine cultured regenerated coronary endothelial cells

Morphological heterogeneity with normal expression but altered function of G proteins in porcine cultured regenerated coronary endothelial cells

Venous Endothelium of Experimental Arteriovenous Fistulas in Rabbits

Myointimal Cells as a Possible Source of Replacement for Endothelial Cells in the Rabbit

Contact Info

Product

Resources

About