Benzyladenine and derivatives-their significance and interconversion in plants

Staden, J. Van; Crouch, Neil R.

doi:10.1007/bf00024582

Cited by 52 publications

(26 citation statements)

References 217 publications

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“…Adenine sulfate is a substrate for the synthesis of natural cytokinins [18]; its beneficial effect on the axillary budding from nodal explants is corroborated by the work on the in vitro organogenesis. Its absence in the optimal IM medium caused a reduction in the number of buds formed by aggregate (data not shown).…”

Section: Discussionmentioning

confidence: 89%

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l -1 citric acid, 12.2 mg.l -1 adenine sulfate, 15 mg.l -1 L-arginine, 2.46 M IBA (indole-3-butyric acid), 30 g.l -1 sucrose and 7 g.l -1 of agar, and enriched with different balances of BA (benzyladenine) and L-glutamine. The histological studies performed on aggregates showed that the buds result from both the development of axillary buds and adventitious budding starting from underlying tissues of the explant. The culture medium containing 6.65 M BA and 25 mg.l -1 L-glutamine gave the best results with an average of 64 buds per aggregate after three weeks for all accessions tested. The buds developed into shoots when placed in an MS medium supplemented with 2.21 M BA, 5.70 M IAA (indole-3-acetic acid) and 15 mg.l -1 L-arginine. These shoots were isolated and then rooted in MS containing 2.46 M of IBA, 2% sucrose and 0.7% agar. The entire process took 13 weeks with a 98% survival rate in terms of plantlets acclimatization. We obtained a multiplication rate of 13 buds per explant and per subculture which is the double of those obtained in other recent works based on the micropropagation of J. curcas from node explants. This protocol is economically more profitable.

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Section: Discussionmentioning

confidence: 89%

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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“…Cytokinins represent an important class of plant hormones that affect cell growth, cell proliferation, cell differentiation, chlorophyll senescence, and apical dominance (reviewed by Mok and Martin 1994). Although the biochemical and physiological effects of cytokinins are well documented, and structure-activity patterns have been already established, the detailed mechanisms of their actions remain unknown (van Staden and Crouch 1996;Prinsen et al 1997). Still, it is currently believed that the free cytokinin base is the active form while other derivatives are considered as cytokinin metabolites.…”

Section: Introductionmentioning

confidence: 97%

“…N-Conjugates, comprising N-alanyl conjugates and N-glucosides, are regarded as detoxification or inactivation products. O-Conjugates are represented mainly by O-glycosides, which are considered to be the transport and storage form of cytokinins (van Staden and Crouch 1996;Zazˇimalova´et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Activation of caspase-like proteases and induction of apoptosis by isopentenyladenosine in tobacco BY-2 cells

Mlejnek

Procházka

2002

Planta

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The effects of isopentenyladenosine (iPA) on tobacco (Nicotiana tabacum L.) BY-2 cells were examined. The number of BY-2 cells decreased in a time- and concentration-dependent manner after being exposed to micromolar concentrations of iPA. This decrease was mainly due to a loss of cell viability, since no substantial changes in cell cycle progression were revealed by flow-cytometric analysis. Dying cells exhibited the typical morphological and biochemical hallmarks of apoptosis, including cell shrinkage, chromatin condensation, and degradation of nuclear DNA to nucleosomal size fragments. Caspase-1-like and caspase-3-like proteases also became activated, the former being dominant. Inhibitor-sensitivity studies revealed that although synthetic caspase inhibitors failed to prevent cell death they markedly reduced cell death in tobacco BY-2 cells, Nu-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone, a specific inhibitor for caspase-1, being the most effective. Our results indicate that caspase-like proteases, and particularly caspase-1-like protease, might be critically implicated in iPA-induced apoptosis of BY-2 cells. Finally, the outcome of inhibiting adenosine kinase by 4-amino-3-iodo-1(beta- D-ribofuranosyl)pyrazolo[3,4-d]-pyrimidine revealed that intracellular phosphorylation of iPA is required for its cytotoxicity to develop.

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“…ML-1 binds with high affinity the natural adenine-related cytokinins: N 6 -isopenthenyladenine (K D = 0.9 ± 0.2 µM) and zeatin (K D = 0.7 ± 0.2 µM). Additionally, ML-1 interacts with BAP (K D = 1.7 ± 0.7 µM), and kinetin (K D = 0.8 ± 0.1 µM), earlier known as synthetic cytokinins but currently considered as natural ones (1,9,10).…”

Section: Resultsmentioning

confidence: 99%