Benzoylecgonine exposure induced oxidative stress and altered swimming behavior and reproduction in Daphnia magna

Parolini, Marco; Felice, Beatrice De; Ferrario, Claudia; Salgueiro‐González, Noelia; Castiglioni, Sara; Finizio, Antonio; Tremolada, Paolo

doi:10.1016/j.envpol.2017.09.038

Cited by 75 publications

(43 citation statements)

References 62 publications

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“…Nunes et al [66] previously reported that no significant effect on GPx activities was observed in A. salina after exposure to sodium dodecyl sulfate and diazepam, whereas the exposure to clofibric acid and clofibrate reduced the activity as test concentration increased. Nevertheless, exposure of D. magna to benzoylecgonine (cocaine metabolite) increased the GPx activity by 1.7-fold and raised the swimming activity [68].…”

Section: Glutathione Peroxidasementioning

confidence: 99%

“…Similarly, tramadol enhanced GPx activity in our bioassays at warmer conditions, but it reduced the overall swimming activity of A. salina. Not only the contaminant, but also its metabolites (e.g., cocaine metabolites) are involved in the activation of redox cycles, depletion, and decrease of antioxidant enzymes leading to the overproduction of reactive oxygen species and oxidative stress conditions [68]. For this reason, it would be fascinating to evaluate in future studies the metabolization of tramadol in A. salina and the possible effects of its metabolites (O-desmethyltramadol and N-desmethyltramadol) on survival and enzymatic.…”

Section: Lactate Dehydrogenasementioning

confidence: 99%

“…However, time of exposure and temperature clearly influenced the impacts (i.e., hydrochlorothiazide at room temperature and 28 °C, after 24-and 48-h exposure). The changes observed for AChE in the first 24 h may be related to the deposition/metabolization rate of the pollutant in the tissue, enzymatic restoration after pollutant excretion, and the impact of the metabolites produced for each substance during the experiments [68,76].…”

Section: Acetylcholinesterasementioning

confidence: 99%

“…Regarding the influence of temperature on contaminants effects, it was observed that AChE was in general reduced (10-74%) in the test treatments at 28 • C in comparison with those exposed to the same contaminants at room temperature. Reduction in AChE activity in different aquatic species (crustaceans, mollusks, and fish) has been reported after exposure to different contaminants, such as benzoylecgonine [68], acetaminophen [75], fluoxetine [76], among others. However, an increment in AChE has also been reported at high-temperature conditions [77].…”

Section: Acetylcholinesterasementioning

confidence: 99%

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Monitoring and Ecotoxicity Assessment of Emerging Contaminants in Wastewater Discharge in the City of Prague (Czech Republic)

Diaz-Sosa

Tapia‐Salazar

Wanner

et al. 2020

Water

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Emerging contaminants (ECs) are not monitored nor regulated consistently, but may have negative effects on human health and ecosystem balance. Although pharmaceuticals and personal care products are among the main ECs found in surface and wastewater, their toxicity and fate are currently not sufficiently studied. In this study, we analyzed for the first time a group of 46 ECs in the secondary effluent of the wastewater treatment plants (WWTP) of Prague. Thirty-seven compounds were identified in the discharge to surface water. Three compounds had no toxicology information on Artemia salina: furosemide, hydrochlorothiazide, and tramadol. We performed acute toxicity (LC50) tests and enzyme assays after 24 and 48 h at room temperature and 28 °C for these three compounds. LC50 ranged from 225.01 mg/L for furosemide, the most toxic, up to above 14,000 mg/L for tramadol. Changes in enzymatic activity for GST, GPx, AChE, and LDH when A. salina were exposed to LC25 for each contaminant were conspicuous and significant in a contaminant-, exposure time-, and temperature-dependent manner. These biochemical markers complement the toxicity profile of these contaminants in aquatic ecosystems and highlight the need for further research on other ECs and their implications, and the regulations required to protect human and ecological health.

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Section: Glutathione Peroxidasementioning

confidence: 99%

Section: Lactate Dehydrogenasementioning

confidence: 99%

Section: Acetylcholinesterasementioning

confidence: 99%

Section: Acetylcholinesterasementioning

confidence: 99%

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Monitoring and Ecotoxicity Assessment of Emerging Contaminants in Wastewater Discharge in the City of Prague (Czech Republic)

Diaz-Sosa

Tapia‐Salazar

Wanner

et al. 2020

Water

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“…The ability to move and escape from contaminants might determine the spatial distribution of organisms and the migration behavior (Gray ; Åtland and Barlaup ; Hansen et al ) and, consequently, affect the ecosystem's dynamics and functioning (Hidaka and Tatsukawa ; Schiesari et al ). For instance, behavior related to movement patterns has been used with Daphnia magna as an early warning of environmental disturbance by online biomonitoring (Ren et al ; Cano et al ; Parolini et al ). For snails, behavioral endpoints such as activity (time to start a normal movement), immobility (Alonso and Camargo ), burying (Hampel et al ), and retraction within the shell (Hellou et al ) have been used as ecotoxicological responses.…”

Section: Spatial Avoidance and Habitat Preference Versus Behavioral Ementioning

confidence: 99%

Spatial avoidance as a response to contamination by aquatic organisms in nonforced, multicompartmented exposure systems: A complementary approach to the behavioral response

Araújo¹,

Blasco²

2018

Enviro Toxic and Chemistry

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The idea that the hazard of contaminants is exclusively related to their toxic effects does not consider the fact that some organisms can avoid contamination, preventing toxicity. Although inferences about avoidance are made in most behavioral ecotoxicology studies, assessment of the real spatial displacement (organisms moving toward another habitat to escape contamination) is difficult due to the type of exposure (confined and mandatory) used in the bioassays: a forced exposure approach. A complementary approach using nonforced exposure systems to assess how contaminants affect the spatial distribution of organisms in a bicompartmented (toxic or nontoxic) environment has long been described. Recently, this nonforced approach has been developed to include a multi compartmented system in which different samples can be simultaneously tested. The aim of the present review was to describe the importance of the nonforced, multicompartmented exposure approach to simulate a gradient or patches of contamination, to describe the 2 main exposure systems, and to highlight the ecological relevance of including spatial avoidance and habitat preference in ecotoxicological studies. The multicompartmentalization of the system makes it possible to simulate more complex scenarios and therefore include new ecological concepts in bioassays. We also contrasted spatial avoidance in the nonforced exposure systems with the behavioral endpoints measured under other exposure systems. Finally, we showed that the nonforced, multicompartmented exposure approach makes it possible 1) to improve environmental risk assessments by adding the dispersion pattern of organisms in a multihabitat scenario, and 2) to integrate ecological concepts such as recolonization of recovering habitats, loss of habitat connectivity, habitat fragmentation, and contamination-driven metapopulation, which have received limited attention in ecotoxicological studies. Environ Toxicol Chem 2019;38:312-320. C 2018 SETAC

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Computational Design and Crystal Structure of a Highly Efficient Benzoylecgonine Hydrolase

et al. 2021

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Benzoylecgonine (BZE) is the major toxic metabolite of cocaine and is responsible for the long‐term cocaine‐induced toxicity owing to its long residence time in humans. BZE is also the main contaminant following cocaine consumption. Here, we identified the bacterial cocaine esterase (CocE) as a BZE‐metabolizing enzyme (BZEase), which can degrade BZE into biological inactive metabolites (ecgonine and benzoic acid). CocE was redesigned by a reactant‐state‐based enzyme design theory. An encouraging mutant denoted as BZEase2, presented a >400‐fold improved catalytic efficiency against BZE compared with wild‐type (WT) CocE. In vivo, a single dose of BZEase2 (1 mg kg−1, IV) could eliminate nearly all BZE within only two minutes, suggesting the enzyme has the potential for cocaine overdose treatment and BZE elimination in the environment by accelerating BZE clearance. The crystal structure of a designed BZEase was also determined.

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Benzoylecgonine exposure induced oxidative stress and altered swimming behavior and reproduction in Daphnia magna

Cited by 75 publications

References 62 publications

Monitoring and Ecotoxicity Assessment of Emerging Contaminants in Wastewater Discharge in the City of Prague (Czech Republic)

Monitoring and Ecotoxicity Assessment of Emerging Contaminants in Wastewater Discharge in the City of Prague (Czech Republic)

Spatial avoidance as a response to contamination by aquatic organisms in nonforced, multicompartmented exposure systems: A complementary approach to the behavioral response

Computational Design and Crystal Structure of a Highly Efficient Benzoylecgonine Hydrolase

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