Benzoxazolin-2(<i>3H</i>)-one inhibits soybean growth and alters the monomeric composition of lignin

Parizotto, Ângela Valderrama; Bubna, Gisele Adriana; Marchiosi, Rogério; Soares, Anderson Ricardo; Ferrarese, Maria de Lourdes Lúcio; Ferrarese-Filho, Osvaldo

doi:10.4161/15592324.2014.989059

Cited by 5 publications

(6 citation statements)

References 28 publications

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“…The obtained dry matter (after overnight incubation at 60 °C) was defined as the protein-free cell wall fraction. The lignin content was determined using the acetyl bromide method [34,35]. Five mg protein-free cell wall fraction were incubated with 0.5 mL 25% acetyl bromide ( v / v in glacial acetic acid) at 70 °C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Root Transcriptomic Analysis Reveals Global Changes Induced by Systemic Infection of Solanum lycopersicum with Mild and Severe Variants of Potato Spindle Tuber Viroid

Góra-Sochacka

Więsyk

Fogtman

et al. 2019

Viruses

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Potato spindle tuber viroid (PSTVd) causes systemic infection in plant hosts. There are many studies on viroid-host plant interactions, but they have predominantly focused on the aboveground part of the plant. Here, we investigated transcriptomic profile changes in tomato roots systemically infected with mild or severe PSTVd variants using a combined microarray/RNA-seq approach. Analysis indicated differential expression of genes related to various Gene Ontology categories depending on the stage of infection and PSTVd variant. A majority of cell-wall-related genes were down-regulated at early infection stages, but at the late stage, the number of up-regulated genes increased significantly. Along with observed alterations of many lignin-related genes, performed lignin quantification indicated their disrupted level in PSTVd-infected roots. Altered expression of genes related to biosynthesis and signaling of auxin and cytokinin, which are crucial for lateral root development, was also identified. Comparison of both PSTVd infections showed that transcriptional changes induced by the severe variant were stronger than those caused by the mild variant, especially at the late infection stage. Taken together, we showed that similarly to aboveground plant parts, PSTVd infection in the underground tissues activates the plant immune response.

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Section: Methodsmentioning

confidence: 99%

Root Transcriptomic Analysis Reveals Global Changes Induced by Systemic Infection of Solanum lycopersicum with Mild and Severe Variants of Potato Spindle Tuber Viroid

Góra-Sochacka

Więsyk

Fogtman

et al. 2019

Viruses

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“…BOA treatment is known to enhance cell wall rigidity. 36 The above described alterations in carbohydrate-related enzymes support the assumption that BOA interferes in the cell wall glucan metabolism and favors lignifications. An intensified glucose release should be correlated to a change in the overall composition of the root cell wall polymers.…”

Section: Compositional Analysis Of the Cell Wallmentioning

confidence: 67%

“…In soybean, BOA increases the production of lignin monomers and the degree of polymerization, leading to higher lignin contents. 36,37 In maize root tips, the enhanced rigidity seems to be more the result of a changed ratio of cell wall carbohydrates to lignin. Deposition of BOA and a second fixation of BOA-Nglucosylated detoxification products within the cell wall of older root parts may contribute to the enhanced rigidity.…”

Section: Discussionmentioning

confidence: 99%

Benzoxazolinone detoxification byN-Glucosylation: The multi-compartment-network ofZea maysL.

Schulz¹,

Filary²,

Kühn³

et al. 2016

Plant Signaling & Behavior

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The major detoxification product in maize roots after 24 h benzoxazolin-2(3H)-one (BOA) exposure was identified as glucoside carbamate resulting from rearrangement of BOA-N-glucoside, but the pathway of N-glucosylation, enzymes involved and the site of synthesis were previously unknown. Assaying whole cell proteins revealed the necessity of H2O2 and Fe(2+) ions for glucoside carbamate production. Peroxidase produced BOA radicals are apparently formed within the extraplastic space of the young maize root. Radicals seem to be the preferred substrate for N-glucosylation, either by direct reaction with glucose or, more likely, the N-glucoside is released by glucanase/glucosidase catalyzed hydrolysis from cell wall components harboring fixed BOA. The processes are accompanied by alterations of cell wall polymers. Glucoside carbamate accumulation could be suppressed by the oxireductase inhibitor 2-bromo-4´-nitroacetophenone and by peroxidase inhibitor 2,3-butanedione. Alternatively, activated BOA molecules with an open heterocycle may be produced by microorganisms (e.g., endophyte Fusarium verticillioides) and channeled for enzymatic N-glucosylation. Experiments with transgenic Arabidopsis lines indicate a role of maize glucosyltransferase BX9 in BOA-N-glycosylation. Western blots with BX9 antibody demonstrate the presence of BX9 in the extraplastic space. Proteomic analyses verified a high BOA responsiveness of multiple peroxidases in the apoplast/cell wall. BOA incubations led to shifting, altered abundances and identities of the apoplast and cell wall located peroxidases, glucanases, glucosidases and glutathione transferases (GSTs). GSTs could function as glucoside carbamate transporters. The highly complex, compartment spanning and redox-regulated glucoside carbamate pathway seems to be mainly realized in Poaceae. In maize, carbamate production is independent from benzoxazinone synthesis.

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“…For Khan et al (2015), lower lignin content in corn is associated with undesirable agronomic traits such as lower yield and higher susceptibility to stem lodging. According to Parizotto et al (2015), excessive lignification may be undesirable, considering that this phenolic compound is related to the lower extensibility of cell walls, which may reduce plant growth. The mean values found in the present study are within the range indicated by Ribeiro Júnior et al (2009) between 1.5 and 7.5%.…”

Section: Discussionmentioning

confidence: 99%

Production of Pearl Millet Irrigated with Different Levels of Brackish Water and Organic Matter

Lira

Andrade

Magalhães

et al. 2020

Communications in Soil Science and Plant Analysis

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The objective is to determine the growth, yield and chemical characteristics of pearl millet irrigated with different levels of brackish water and organic matter in two cultivation cycles. The experimental design was randomized blocks in a 4 × 4 factorial arrangement, composed of 4 levels of brackish irrigation (25, 50, 75 and 100% evapotranspiration), 4 levels of organic matter (0, 15, 30 and 45 t ha −1 ) with 3 repetitions. Along two cultivation cycles, pearl millet crop was analyzed for: plant growth variables, fresh and dry mass production, water-use efficiency, and chemical composition. There was significant interaction between applied water levels and organic matter for lignin in the 2nd cut (P < .05). The number of leaves, number of dead leaves and fresh mass production were influenced by the isolated effect of brackish water levels in cut 1 (P < .05). In turn, fresh mass production, dry mass production, plant height, leaves length, panicle length, fresh mass production, crude protein and ether extract were influenced by the isolated effect of saline water levels in the 2nd cut (P < .05). Growth, biomass production and chemical composition variables in second cut are positively influenced by different brackish irrigation levels under low rainfall conditions.

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Benzoxazolin-2(3H)-one inhibits soybean growth and alters the monomeric composition of lignin

Cited by 5 publications

References 28 publications

Root Transcriptomic Analysis Reveals Global Changes Induced by Systemic Infection of Solanum lycopersicum with Mild and Severe Variants of Potato Spindle Tuber Viroid

Root Transcriptomic Analysis Reveals Global Changes Induced by Systemic Infection of Solanum lycopersicum with Mild and Severe Variants of Potato Spindle Tuber Viroid

Benzoxazolinone detoxification byN-Glucosylation: The multi-compartment-network ofZea maysL.

Production of Pearl Millet Irrigated with Different Levels of Brackish Water and Organic Matter

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