Benzothiophene and dibenzothiophene as the sole sulfur source in Acinetobacter: growth kinetics and oxidation products

Marzoná, M.; Pessione, Enrica; Martino, Silvana; Giunta, Carlo

doi:10.1016/s0378-3820(97)00029-5

Cited by 18 publications

(5 citation statements)

References 17 publications

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“…Similar growth inhibition behavior with 2-HBP production was also observed in previous BDS studies. 25,26 It was reported that 2-HBP above 200 µ mol/L was toxic to the bacterial cells and inhibitory to biodesulfurization. 8 Even though the maximum levels of 2-HBP concentration produced in our studies were not close to the toxic level, a decrease in 2-HBP production rate was observed with cell death.…”

Section: Dbt Consumption Kinetics By S Solfataricus P2mentioning

confidence: 99%

“…Sulfur-free mineral (SFM) medium was prepared by dissolving 70 mg of CaCl 2 .2H 2 O, 1.3 g of NH 4 Cl, 0.25 g of MgCl 2 .6H 2 O, 0.28 g of KH 2 PO 4 , and 0.5 mL of trace elements solution in 1 L of Milli-Q water, and this mixture was adjusted to pH 3 with HCl. Trace elements solution18 was prepared with25 g L −1 EDTA, 2.14 g L −1 ZnCl 2 , 2.5 g L −1 MnCl 2 .4H 2 O, 0.3 g L −1 CoCl 2 .6H 2 O, 0.2 g L −1 CuCl 2 .2H 2 O, 0.4 g L −2H 2 O, 4.5 g L −1 CaCl 2 .2H 2 O, 2.9 g L −1FeCl 3 .6H 2 O, 1.0 g L −1 H 3 BO 3 , and 0.1 g L −1 KI.…”

mentioning

confidence: 99%

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Revisiting the biodesulfurization capability of hyperthermophilic archaeon Sulfolobus solfataricus P2 revealed DBT consumption by the organism in an oil/water two-phase liquid system at high temperatures

Gün¹,

Yürüm²,

Doğanay³

2015

Turk J Chem

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Abstract:The ability of the hyperthermophilic archaeon Sulfolobus solfataricus P2 to grow on organic and inorganic sulfur sources was investigated. A sulfur-free mineral medium was employed with different sources of carbon. The results showed that inorganic sulfur sources display growth curve patterns significantly different from the curves obtained with organic sulfur sources. Solfataricus has the ability to utilize DBT and its derivatives, but it lacks BT utilization.Solfataricus utilizes DBT at a rate of 1.23 µ mol 2-HBP h −1 g DCW −1 even at 78• C, at which DBT is known to be unstable. After enabling DBT stabilization using a two-phase culture system, stable microbial growth was achieved showing a desulfurization rate of 0.34 µ M DBT g DCW −1 h −1 . Solfataricus offers beneficial properties compared to the other desulfurizing mesophilic/moderate thermophilic bacteria due to its capacity to utilize DBT and its derivatives under hyperthermophilic conditions.

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Section: Dbt Consumption Kinetics By S Solfataricus P2mentioning

confidence: 99%

mentioning

confidence: 99%

Revisiting the biodesulfurization capability of hyperthermophilic archaeon Sulfolobus solfataricus P2 revealed DBT consumption by the organism in an oil/water two-phase liquid system at high temperatures

Gün¹,

Yürüm²,

Doğanay³

2015

Turk J Chem

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“…It has been demonstrated that some organosulfur compounds can also serve as a sulfur source for microbial growth (Alves et al, 2005;Marzona et al, 1997;Van Hamme et al, 2004). Thus, in sulfur-deficient environments, the release of sulfur from benzothiophenes could provide this essential growth element to microorganisms.…”

Section: Introductionmentioning

confidence: 99%

“…strain JVH1 with thiophenic sulfur compounds or sulfate as sole sulfur sources and acetate as carbon source (average ± standard deviation, n = 3). Closed and open circles, benzothiophene and benzothiophene sulfone, 0.1 mmol l -1 ; closed and open triangles, dibenzothiophene and dibenzothiophene sulfone, 0.01 mmol l -1 ; closed and open squares, sulfur-free negative controls and positive controls with sulfateChang et al, 1998;Kayser et al, 2002;Marzona et al, 1997;Tanaka et al, 2002)…”

mentioning

confidence: 99%

Sulfur from benzothiophene and alkylbenzothiophenes supports growth of Rhodococcus sp. strain JVH1

et al. 2006

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Rhodococcus sp. strain JVH1 was previously reported to use a number of compounds with aliphatic sulfide bridges as sulfur sources for growth. We have shown that although JVH1 does not use the three-ring thiophenic sulfur compound dibenzothiophene, this strain can use the two-ring compound benzothiophene as its sole sulfur source, resulting in growth of the culture and loss of benzothiophene. Addition of inorganic sulfate to the medium reduced the conversion of benzothiophene, indicating that benzothiophene metabolism is repressed by sulfate and that benzothiophene is therefore used specifically as a sulfur source. JVH1 also used all six isomers of methylbenzothiophene and two dimethylbenzothiophene isomers as sulfur sources for growth. Metabolites identified from benzothiophene and some methylbenzothiophenes were consistent with published pathways for benzothiophene biodesulfurization. Products retaining the sulfur atom were sulfones and sultines, the sultines being formed from phenolic sulfinates under acidic extraction conditions. With 2-methylbenzothiophene, the final desulfurized product was 2-methylbenzofuran, formed by dehydration of 3-(o-hydroxyphenyl) propanone under acidic extraction conditions and indicating an oxygenative desulfination reaction. With 3-methylbenzothiophene, the final desulfurized product was 2-isopropenylphenol, indicating a hydrolytic desulfination reaction. JVH1 is the first microorganism reported to use all six isomers of methylbenzothiophene, as well as some dimethylbenzothiophene isomers, as sole sulfur sources. JVH1 therefore possesses broader sulfur extraction abilities than previously reported, including not only sulfidic compounds but also some thiophenic species.

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“…Pseudomonas (Luo et al, 2003), Rhodococcus (Maghsoudi et al, 2001), Acinetobacter (Marzona et al, 1997), Desulfovibrio (Bahrami et al, 2001).…”

mentioning

confidence: 99%

Oxidación del dibenzotiofeno mediante los sistemas: enzima lacasa-ABTS o ácido fosfotúngstico-peróxido de hidrógeno y desulfuración oxidativa del diesel

Ortega

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Tecnología (CONACYT) y además cuenta con el apoyo del mismo Consejo, con el convenio PFP-20-93.El presente trabajo se llevó a cabo en el laboratorio de Biología Molecular de Hongos Filamentosos del departamento de Biotecnología de la UAM-Iztapalapa y fue parcialmente financiado por el proyecto FIES 98112-2 del Instituto Mexicano del Petróleo.El alumno de Doctorado Francisco Villaseñor Ortega recibió el apoyo económico del CONACyT con el registro de becario número 91901.

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Benzothiophene and dibenzothiophene as the sole sulfur source in Acinetobacter: growth kinetics and oxidation products

Cited by 18 publications

References 17 publications

Revisiting the biodesulfurization capability of hyperthermophilic archaeon Sulfolobus solfataricus P2 revealed DBT consumption by the organism in an oil/water two-phase liquid system at high temperatures

Revisiting the biodesulfurization capability of hyperthermophilic archaeon Sulfolobus solfataricus P2 revealed DBT consumption by the organism in an oil/water two-phase liquid system at high temperatures

Sulfur from benzothiophene and alkylbenzothiophenes supports growth of Rhodococcus sp. strain JVH1

Oxidación del dibenzotiofeno mediante los sistemas: enzima lacasa-ABTS o ácido fosfotúngstico-peróxido de hidrógeno y desulfuración oxidativa del diesel

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