Benzoquinone-mediated enzyme biosensor for amperometric determination of glucose

Abstract

An amperometric enzyme sensor for the analysis of glucose is described. The sensor uses benzoquinone as a mediator for electron transfer between immobilized glucose oxidase and a pyrolytic graphite electrode. A linear current response, proportional to glucose concentration in the range 1-50 mM, is observed. The influence of oxygen, pH and operating potential on the sensor performance is examined.

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“…On the other hand, "proper" mediators are the mediators that possess enhanced electron transfer properties with proximate redox potential compared to enzyme active center redox potential [23,26]. BQ is the one of the most preferred mediator type for GOx catalyzed reactions [27][28][29]. For this reason, in this work BQ was chosen as the suitable mediator.…”

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“…Generally, immobilization of the mediators on the electrode can be achieved through different strategies, such as dispersion of the mediator in the bulk of a composite electrode or adsorption, covalent attachment or entrapment of the mediator on the electrode surface, 15 etc. Benzoquinone and some organic compounds, as redox mediators, due to their neutrality and lack of modiable functional groups, are oen physically absorbed on the electrode for enzyme biosensors 16,17 and therefore the leaching of the mediator from the matrix would potentially diminish the performances of the modied electrodes.…”