Benzoporphyrinderivat-Monosäure zur Photodynamischen Therapie des Endometriums*

Abstract

The aim was to study the impact of photodynamic therapy (PDT) on the endometrium after local intrauterine application of photosensitiser (Ps) and laser light without sensitising the skin. To our knowledge Benzoporphyrin Derivative Mono Acid (BPD) was used for the first time for this purpose in the rat model. The advantage of using BPD is the fact that light of 690 nm (maximum absorption) penetrates deeper into tissue and shows less absorption by haemoglobin. Low-light level tissue fluorescence imaging revealed a distinct positive endometrium-to-myometrium ratio within the first 12 hours. Relative fluorescence was highest in the endometrial glands and lowest in the myometrium. After 12 hours the intensity of fluorescence levelled off in all compartments and values of the glands approached close to those of the other layers. REPRODUCTIVE PERFORMANCE AFTER PDT: There was a significant difference in nidations (p < 0.03) in the treated left uterine horn as compared to the untreated right horn and the control animals (light/no drug and drug/no light). HISTOLOGICAL CHANGES: Following PDT a marked atrophy of the endometrial layer was observed in most animals leaving just a single cell epithelial layer covering the myometrium. SKIN PHOTOSENSITIVITY: None of the animals showed any alterations in the light treated skin area at any time. These promising results show that PDT of the endometrium is possible after topical application of the photosensitising drug and the laser light without provoking skin sensitivity.

Photodynamic detection of diseased axillary sentinel lymph node after oral application of aminolevulinic acid in patients with breast cancer

“…Topical ALAbased PDT has been reported effective for the treatment of a variety of dermatological diseases including cutaneous superficial and nodular basal cell carcinoma, Bowen's disease and actinic (solar) keratoses (Morton et al, 2001). Local intrauterine application of ALA has also been used for selective endometrial ablation in animal models and in human clinical studies (Steiner et al, 1995(Steiner et al, , 1996bWyss et al, 2001). By the use of blue light at 400 nm, PpIX induced by ALA has been applied as a fluorescence detection marker for photodiagnosis of cancer and dysplastic conditions of the urinary bladder and other organs (Kennedy et al, 1996).…”