Benzoate-Tolerant<i>Escherichia coli</i>Strains from Experimental Evolution Lose the Gad Regulon, Multi-Drug Efflux Pumps, and Hydrogenases

Moore, Jeremy P.; Li, Haofan; Engmann, Morgan L.; Bischof, Katarina M.; Kunka, Karina S; Harris, Mary E.; Tancredi, Anna C.; Ditmars, Frederick S.; Basting, Preston J.; George, Nadja S.; Bhagwat, Arvind A.; Slonczewski, Joan L.

doi:10.1101/531178

Cited by 3 publications

(2 citation statements)

References 102 publications

(158 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutation distributions of the minichromosome replicons showed no significant difference in ISH proportion (94 mutations out of 111, on pNRC100; 141 out of 156 on pNRC200). For comparison, we considered a recent breseq analysis of 16 E. coli genomes following 500 generations evolution with an organic acid (Moore et al, 2019). Only 18% of the E. coli mutations were mediated by insertion sequences.…”

Section: Mutations In the Genomes From Evolving Populationsmentioning

confidence: 99%

“…However, 2,000 generations of E. coli evolution at pH 4.8 select for loss of three acid-inducible amino-acid decarboxylase systems, including arginine decarboxylase (He et al, 2017). As a membrane-permeant acid, benzoic acid induces glutamate decarboxylase and drug resistance regulons, yet these systems are lost or downregulated during experimental evolution (Creamer et al, 2017;Moore et al, 2019). At high external pH, E. coli survival requires the stress sigma factor RpoS; however, generations of growth at high pH select against RpoS expression and activity (Hamdallah et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acid Experimental Evolution of the Haloarchaeon Halobacterium sp. NRC-1 Selects Mutations Affecting Arginine Transport and Catabolism

et al. 2020

Self Cite

View full text Add to dashboard Cite

Halobacterium sp. NRC-1 (NRC-1) is an extremely halophilic archaeon that is adapted to multiple stressors such as UV, ionizing radiation and arsenic exposure; it is considered a model organism for the feasibility of microbial life in iron-rich brine on Mars. We conducted experimental evolution of NRC-1 under acid and iron stress. NRC-1 was serially cultured in CM + medium modified by four conditions: optimal pH (pH 7.5), acid stress (pH 6.3), iron amendment (600 µM ferrous sulfate, pH 7.5), and acid plus iron (pH 6.3, with 600 µM ferrous sulfate). For each condition, four independent lineages of evolving populations were propagated. After 500 generations, 16 clones were isolated for phenotypic characterization and genomic sequencing. Genome sequences of all 16 clones revealed 378 mutations, of which 90% were haloarchaeal insertion sequences (ISH) and ISH-mediated large deletions. This proportion of ISH events in NRC-1 was five-fold greater than that reported for comparable evolution of Escherichia coli. One acid-evolved clone had increased fitness compared to the ancestral strain when cultured at low pH. Seven of eight acid-evolved clones had a mutation within or upstream of arcD, which encodes an arginine-ornithine antiporter; no non-acid adapted strains had arcD mutations. Mutations also affected the arcR regulator of arginine catabolism, which protects bacteria from acid stress by release of ammonia. Two acid-adapted strains shared a common mutation in bop, which encodes bacterio-opsin, apoprotein for the bacteriorhodopsin light-driven proton pump. Thus, in the haloarchaeon NRC-1, as in bacteria, pH adaptation was associated with genes involved in arginine catabolism and proton transport. Our study is among the first to report experimental evolution with multiple resequenced genomes of an archaeon. Haloarchaea are polyextremophiles capable of growth under environmental conditions such as concentrated NaCl and desiccation, but little is known about pH stress. Interesting parallels appear between the molecular basis of pH adaptation in NRC-1 and in bacteria, particularly the acid-responsive arginine-ornithine system found in oral streptococci.

show abstract

Section: Mutations In the Genomes From Evolving Populationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Acid Experimental Evolution of the Haloarchaeon Halobacterium sp. NRC-1 Selects Mutations Affecting Arginine Transport and Catabolism

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

Four LysR-type transcriptional regulator family proteins (LTTRs) involved in antibiotic resistance in Aeromonas hydrophila

Cai

Wang

et al. 2019

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Acid Experimental Evolution of the Extremely Halophilic Archaeon Halobacterium sp. NRC-1 Selects Mutations Affecting Arginine Transport and Catabolism

Kunka

Griffith

Holdener

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

16Halobacterium sp. NRC-1 (NRC-1) is an extremely halophilic archaeon that is well 17 adapted to multiple stressors such as UV, ionizing radiation, and arsenic exposure. We conducted 18 experimental evolution of NRC-1 under acid and iron stress to expand the stressors. NRC-1 was 19 serially cultured in CM+ medium modified by four stress conditions, with four evolving 20 populations per condition. At 500 generations the conditions were: optimal pH (pH 7.5), acid 21 stress (pH 6.3), iron stress (600 μM ferrous sulfate, pH 7.5), and acid plus iron stress (600 μM 22

show abstract

Benzoate-TolerantEscherichia coliStrains from Experimental Evolution Lose the Gad Regulon, Multi-Drug Efflux Pumps, and Hydrogenases

Abstract: 19

Cited by 3 publications

References 102 publications

Acid Experimental Evolution of the Haloarchaeon Halobacterium sp. NRC-1 Selects Mutations Affecting Arginine Transport and Catabolism

Acid Experimental Evolution of the Haloarchaeon Halobacterium sp. NRC-1 Selects Mutations Affecting Arginine Transport and Catabolism

Four LysR-type transcriptional regulator family proteins (LTTRs) involved in antibiotic resistance in Aeromonas hydrophila

Acid Experimental Evolution of the Extremely Halophilic Archaeon Halobacterium sp. NRC-1 Selects Mutations Affecting Arginine Transport and Catabolism

Contact Info

Product

Resources

About