Benzo[&lt;i&gt;c&lt;/i&gt;]phenanthridine alkaloids and glycosylated flavonoids from the roots of &lt;i&gt;Zanthoxylum gilletii&lt;/i&gt; (Rutaceae)

Dzouemo, Liliane Clotilde; Happi, Emmanuel Ngeufa; Happi, Gervais Mouthé; Tsopgni, Willifred Dongmo Tekapi; Wansi, Jean Duplex

doi:10.53365/nrfhh/154598

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…The results showed that the plant produces chemical constituents with a high structural diversity from terpenoid derivatives (triterpenoids and steroids) identified as the major classes of compounds to flavonoids and other phenolic compounds. The significant change observed in the core structures from one compound to another is mainly oxidation of some functional groups and it might play an important role in their potencies and the understanding of their action mechanisms (Dzouemo et al, 2022;Happi et al, 2020).…”

Section: Phytochemical Studymentioning

confidence: 99%

Antimicrobial and cytotoxic extractives from Ficus sycomorus L. (Moraceae)

Mandou¹,

Tsopgni²,

Nsangou³

et al. 2022

NRFHH

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Our search for antimicrobial and cytotoxic leads from Ficus sycomorus led to the isolation of twenty-four known compounds (1‒24) from its methanolic stem bark and root extracts. The structures of all the isolated compounds were established using their 1D- and 2D-NMR spectroscopic data while the crude extracts, some fractions and compounds were tested for their antibacterial, antifungal and cytotoxicity activities. In the antimicrobial assay, compound 18 displayed good to moderate activity with MIC values of 31.25 µg/ml against Escherichia coli, 125 µg/ml against Salmonella typhimurium cpc and Pseudomonas aeruginosa, while compounds 10, 13 and 14 demonstrated moderate activities on almost all the tested strains, with the highest potency observed for compound 13 on E. coli (MIC = 125 µg/ml). However, although compounds 18 and 19 gave a significant inhibition of the VERO cell line ATCC CRT-1586 with CC50 values of 52.60 µg/ml and 69.10 µg/ml, respectively, all the extract samples and other tested compounds did not show distinguishable cytotoxicity. Finally, the chemophenetic significance of the isolated compounds is discussed.

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Section: Phytochemical Studymentioning

confidence: 99%

Antimicrobial and cytotoxic extractives from Ficus sycomorus L. (Moraceae)

Mandou¹,

Tsopgni²,

Nsangou³

et al. 2022

NRFHH

Self Cite

View full text Add to dashboard Cite

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Phytochemical analysis, in-vitro and in-silico study of antiproliferative activity of ethyl acetate fraction of Launaea cornuta (Hochst. ex Oliv. & Hiern) C. Jeffrey against human cervical cancer cell line

Lagu,

Nyamai,

Njeru

2024

Front. Pharmacol.

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Introduction: Cervical cancer is one of the leading causes of death among women globally due to the limitation of current treatment methods and their associated adverse side effects. Launaea cornuta is used as traditional medicine for the treatment of a variety of diseases including cancer. However, there is no scientific validation on the antiproliferative activity of L. cornuta against cervical cancer.Objective: This study aimed to evaluate the selective antiproliferative, cytotoxic and antimigratory effects of L. cornuta and to explore its therapeutical mechanisms in human cervical cancer cell lines (HeLa-229) through a network analysis approach.Materials and methods: The cytotoxic effect of L. cornuta ethyl acetate fraction on the proliferation of cervical cancer cells was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) bioassay and the antimigratory effect was assessed by wound healing assays. Compounds were analysed using the qualitative colour method and gas chromatography-mass spectroscopy (GC-MS). Subsequently, bioinformatic analyses, including the protein-protein interaction (PPI) network analysis, Gene Ontology (GO), and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analysis, were performed to screen for potential anticervical cancer therapeutic target genes of L. cornuta. Molecular docking (MD) was performed to predict and understand the molecular interactions of the ligands against cervical cancer. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to validate the network analysis results.Results:L. cornuta ethyl acetate fraction exhibited a remarkable cytotoxic effect on HeLa-229 proliferation (IC50 of 20.56 ± 2.83 μg/mL) with a selectivity index (SI) of 2.36 with minimal cytotoxicity on non-cancerous cells (Vero-CCL 81 (IC50 of 48.83 ± 23.02). The preliminary screening revealed the presence of glycosides, phenols, saponins, terpenoids, quinones, and tannins. Thirteen compounds were also identified by GC-MS analysis. 124 potential target genes associated with the effect of L. cornuta ethyl acetate fraction on human cervical cancer were obtained, including AKT1, MDM2, CDK2, MCL1 and MTOR were identified among the top hub genes and PI3K/Akt1, Ras/MAPK, FoxO and EGFR signalling pathways were identified as the significantly enriched pathways. Molecular docking results showed that stigmasteryl methyl ether had a good binding affinity against CDK2, ATK1, BCL2, MDM2, and Casp9, with binding energy ranging from −7.0 to −12.6 kcal/mol. Tremulone showed a good binding affinity against TP53 and P21 with −7.0 and −8.0 kcal/mol, respectively. This suggests a stable molecular interaction of the ethyl acetate fraction of L. cornuta compounds with the selected target genes for cervical cancer. Furthermore, RT-qPCR analysis revealed that CDK2, MDM2 and BCL2 were downregulated, and Casp9 and P21 were upregulated in HeLa-229 cells treated with L. cornuta compared to the negative control (DMSO 0.2%).Conclusion: The findings indicate that L. cornuta ethyl acetate fraction phytochemicals modulates various molecular targets and pathways to exhibit selective antiproliferative and cytotoxic effects against HeLa-229 cells. This study lays a foundation for further research to develop innovative clinical anticervical cancer agents.

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Benzo[<i>c</i>]phenanthridine alkaloids and glycosylated flavonoids from the roots of <i>Zanthoxylum gilletii</i> (Rutaceae)

Cited by 2 publications

References 40 publications

Antimicrobial and cytotoxic extractives from <i>Ficus sycomorus</i> L. (Moraceae)

Antimicrobial and cytotoxic extractives from <i>Ficus sycomorus</i> L. (Moraceae)

Phytochemical analysis, in-vitro and in-silico study of antiproliferative activity of ethyl acetate fraction of Launaea cornuta (Hochst. ex Oliv. & Hiern) C. Jeffrey against human cervical cancer cell line

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