Benzo(<i>a</i>)pyrene induces hepatic AKR1A1 mRNA expression in tilapia fish (<i>Oreochromis niloticus</i>)

Osorio-Yáñez, Citlalli; García-Tavera, José Luis; Pérez-Núñez, M. T.; Poblete-Naredo, Irais; Muñoz, Balam; Barrón-Vivanco, Briscia Socorro; Rothenberg, Stephen J.; Zapata-Pérez, Omar; Albores, Arnulfo

doi:10.3109/15376516.2012.666684

Cited by 6 publications

(3 citation statements)

References 38 publications

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“…A 1.5 μL aliquot of the PCR product was transferred to a new master mixture containing primers. Cycling parameters were the same as described above (Osorio-Yáñez et al 2012).…”

Section: Total Rna Extractionmentioning

confidence: 99%

Ultrastructure and Molecular Toxicological Effects of the Coronate Scyphomedusa Linuche unguiculata Venom on Giardia duodenalis

et al. 2020

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Cnidarian venoms are rising as a new source of potential pharmacologically active molecules. They have been investigated for their neurotoxins acting on several nervous system targets but little is known about their antimicrobial and antiparasitic properties. Giardiasis, a common disease in developing countries, is produced by the protozoan Giardia duodenalis which induces severe gastroenteritis when infecting humans. The aim of this study is to describe the structural and molecular effects of Linuche unguiculata jellyfish extract on G. duodenalis. In this study, the effects on the ultrastructure were observed by electron transmission microscopy 24 h after the exposure to the extract and to the commercial antiparasitic drugs metronidazole, nitazoxanide and albendazole. To assess the effect at the molecular level, the gene expression analysis of β-tubulin, β-giardin, cysteine protease and glutamate dehydrogenase of G. duodenalis exposed to Linuche's extract was performed by the RT-PCR technique. Our results show that the exposure to venom caused severe changes on Giardia's cellular organelles such as submembrane vesicles, nuclei and adhesive disk. Our experiments also showed that the extract significantly alters the expression of genes that encode for the structural protein β-tubulin and the enzyme glutamate dehydrogenase, which are related to cell viability.

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“…A 1.5 μL aliquot of the PCR product was transferred to a new master mixture containing primers. Cycling parameters were the same as described above (Osorio-Yáñez et al 2012).…”

Section: Total Rna Extractionmentioning

confidence: 99%

Ultrastructure and Molecular Toxicological Effects of the Coronate Scyphomedusa Linuche unguiculata Venom on Giardia duodenalis

et al. 2020

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“…Aldehyde reductase (ALR) has been widely characterized in organisms and has been implicated in cellular redox balance (Penning, 2017). ALRs are evolutionarily conserved among species and display similar gene structures and functions (Osorio‐Yáñez et al ., 2012). ALR catalyses the reduction of endogenous and exogenous aldehydes in an NADPH‐dependent manner (Fujii et al ., 2021).…”

Section: Introductionmentioning

confidence: 99%

“…To date, there are few reports on teleost ALRs. In zebrafish, ALR is vital for maintaining glucose balance (Li et al ., 2020), whereas ALR is involved in the detoxification of benzo (a) pyrene in tilapia (Hassanin et al ., 2017; Osorio‐Yáñez et al ., 2012). Administration of recombinant ALR alleviated oxidative damage and reduced immunotoxicity in yellow catfish under cadmium stress (Yang et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

Swamp eel aldehyde reductase is involved in response to nitrosative stress via regulating NO/GSH levels

Zhao

Yang

et al. 2023

Journal of Fish Biology

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Aldehyde reductase (ALR) plays key roles in the detoxification of toxic aldehyde. In this study, the authors cloned the swamp eel ALR gene using rapid amplification of cDNA ends‐PCR (RACE‐PCR). The recombinant protein (rALR) was expressed in Escherichia coli and purified using a Ni2+‐NTA chelating column. The rALR protein exhibited efficient reductive activity towards several aldehydes, ketones and S‐nitrosoglutathione (GSNO). A spot assay suggested that the recombinant E. coli strain expressing rALR showed better resistance to formaldehyde, sodium nitrite and GSNO stress, suggesting that swamp eel ALR is crucial for redox homeostasis in vivo. Consequently, the authors investigated the effect of rALR on the oxidative parameters of the liver in swamp eels challenged with Aeromonas hydrophila. The hepatic glutathione (GSH) content significantly increased, and the hepatic NO content and levels of reactive oxygen species and reactive nitrogen species significantly decreased when rALR was administered. In addition, the mRNA expression of hepatic Alr, HO1 and Nrf2 was significantly upregulated, whereas the expression levels of NF‐κB, IL‐1β and NOS1 were significantly downregulated in the rALR‐administered group. Collectively, these results suggest that ALR is involved in the response to nitrosative stress by regulating GSH/NO levels in the swamp eel.

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The effect of benzo[a]pyrene on the gut microbiota of Nile tilapia (Oreochromis niloticus)

Quintanilla-Mena

Vega-Arreguín

Río-García

et al. 2021

Appl Microbiol Biotechnol

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Benzo(a)pyrene induces hepatic AKR1A1 mRNA expression in tilapia fish (Oreochromis niloticus)

Cited by 6 publications

References 38 publications

Ultrastructure and Molecular Toxicological Effects of the Coronate Scyphomedusa Linuche unguiculata Venom on Giardia duodenalis

Ultrastructure and Molecular Toxicological Effects of the Coronate Scyphomedusa Linuche unguiculata Venom on Giardia duodenalis

Swamp eel aldehyde reductase is involved in response to nitrosative stress via regulating NO/GSH levels

The effect of benzo[a]pyrene on the gut microbiota of Nile tilapia (Oreochromis niloticus)

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