Benzo[a]pyrene inhibits testosterone biosynthesis via NDUFA10-mediated mitochondrial compromise in mouse Leydig cells: Integrating experimental and in silico toxicological approaches

Holistic Assessment Based On Hepatocyte Mitochondria: Lycopene Repairs Oxidized mtDNA to Alleviate Mitochondrial Stress Induced by Atrazine

“…Mitochondrial biogenesis is an essential biological process in mitochondria, and PGC-1α regulates the transcription and expression of TFAM, SIRT1, and SIRT3, which maintains mitochondrial homeostasis. 37,38 In the present study, changes in mitochondrial biogenesis due to the toxic effects of ATZ were found. It has been shown that the loss of mitochondrial function decreases sperm motility and causes cellular oxidative stress.…”

Leveraging integrative toxicogenomic approach towards development of stressor-centric adverse outcome pathway networks for plastic additives

“…An AOP network constructed from stressor-specific AOPs can highlight interactions among the associated AOPs, thereby aiding in the assessment of stressor-induced toxicity (Chai et al, 2021; Pogrmic-Majkic et al, 2022; Rugard et al, 2020; Sahoo et al, 2024; Yang et al, 2022). Among the 15 plastic additives, we observed that Benzo[a]pyrene (B[a]P or CAS:50-32-8) has the largest number of associated AOPs (28 AOPs), all of which were solely identified through our systematic data integrative approach (Table 1).…”

“…Previously, Yang et al . (Yang et al, 2022) had constructed an AOP network for B[a]P-induced toxicity, but they relied only on CTD to identify KEs associated with B[a]P and focused only on B[a]P-induced male reproductive damages. Therefore, we relied on 28 AOPs associated with B[a]P-induced toxicity (which we designate as B[a]P-AOPs) and constructed an AOP network to explore various adverse effects associated with B[a]P.…”

“…These 28 AOPs are classified under various disease categories namely, cancer, gastrointestinal system disease, reproductive system disease, respiratory system disease, cognitive disorder, thoracic disease, and musculoskeletal system disease (Supplementary Table S9). Previously, Yang et al (Yang et al, 2022) Next, we computed the cumulative weight of evidence (WoE) for each of these 28 B[a]P-AOPs based on their KER information to assess the biological plausibility (Ravichandran et al, 2022b;Sahoo et al, 2024). We observed that 9 of these 28 B[a]P-AOPs have 'High' cumulative WoE and 6 B[a]P-AOPs have 'Moderate' cumulative WoE (Supplementary Table S11).…”

“…Notably, we observed that AOP:263, AOP:264, AOP:265, AOP:267, and AOP:268 are shared among all 15 plastic additives (Table 1). Moreover, we observed that these five AOPs An AOP network constructed from stressor-specific AOPs can highlight interactions among the associated AOPs, thereby aiding in the assessment of stressor-induced toxicity (Chai et al, 2021;Pogrmic-Majkic et al, 2022;Rugard et al, 2020;Sahoo et al, 2024;Yang et al, 2022). Among the 15 plastic additives, we observed that Benzo[a]pyrene (B[a]P or (which was not certified by peer review) is the author/funder.…”