Benzo[a]pyrene-induced elevation of GSH level protects against oxidative stress and enhances xenobiotic detoxification in human HepG2 cells

Lin, Tao; Yang, M. S.

doi:10.1016/j.tox.2007.03.002

Cited by 71 publications

(52 citation statements)

References 62 publications

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“…Hypercholesterolemia is known to induce oxidative stress and lipid peroxidation (Lee et al 2013). GSH plays important role against toxicity and participate in the remove reactive metabolites by decreasing hydroperoxides in the existence (Nicotera and Orrenius 1986, Marinho et al 1997, Lin and Yang 2007. CAT, SOD and GPx are formed to protect cell membranes and intracellular materials from reactive oxygen species, including free radicals.…”

Section: Discussionmentioning

confidence: 99%

Effects of ginseng on antioxidant enzymes in rats fed cholesterol rich diet

Uluişik¹,

Keskin²

2016

EurasianJVetSci

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Yüksek kolesterollü diyetle beslenen ratlarda ginsengin antioksidan enzimler üzerine etkileriÖz Amaç: Bu çalışmada yüksek kolesterollü diyetle beslenen ratlarda ginsengin antioksidan enzimler üzerine etkilerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem:Çalışmada kullanılan hayvanlar kontrol, kolesterol ve kolesterol + ginseng grubu olarak üç eşit gruba ayrıldı. Kontrol grubu 40 gün süre ile standart rat yemi ile ad libitum olarak beslendi. Kolesterol grubu %5 kolesterol içeren yemle beslenirken, kolesterol + ginseng grubu ise %5 kolesterol ve 1 g/kg panax ginseng kök tozu içeren aynı yemle beslendi. Çalışmanın 40. gününde bütün gruplardaki hayvanlardan kan örnekleri alındı. Alınan kanların plazmalarında tiyobarbitürik asit reaktif ürünleri (TBARS), süperok-sit dismutaz (SOD), glutatyon (GSH), glutatyon peroksidaz (GPx) ve katalaz (CAT) düzeyleri belirlendi. Bulgular: Çalışmada kolesterollü diyetle beslenen gruptaki hayvanlarda belirlenen GSH ve SOD düzeylerinin kontrol grubu değerleri ile karşılaştırıldığında anlamlı olarak azaldığı gözlenirken (P<0.05), aynı parametrelerin kolesterol + ginseng grubunda kolesterol ve kontrol grupları değerlerin-den farksız oldukları belirlendi. Çalışmada TBARS, GPx ve CAT düzeyleri bakımından gruplar arası herhangi önemli bir farklılığın meydana gelmediği görüldü.Öneri: Elde edilen sonuçlara dayanarak hiperkolesterolemiye bağlı gelişen oksidatif stres riski üzerine panax ginsengin yararlı etkilerinin olabileceği kanaatine varıldı. Anahtar kelimeler: Kolesterol, ginseng, oksidatif stres AbstractAim: In this study, it was aimed to investigate the effects of ginseng on antioxidant enzymes in rats fed cholesterol rich diet. Materials and Methods:Rats were equally divided into three groups: control group, cholesterol group and cholesterol + ginseng group. The control group had fed as ad libitum with a standard rat diet for 40 days. The cholesterol and cholesterol + ginseng groups had ad libitum access to the same diet containing 5% cholesterol powder and 5% cholesterol + 1 g/kg panax ginseng root powder, respectively, for 40 days. On the 40th day of the study, blood samples were taken from all animals in each group. In plasma samples, thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT) levels were determined.Results: GSH and SOD levels significantly decreased with feeding high cholesterol diet compared to control group (P<0.05), while these parameters in cholesterol + ginseng group were not different from both cholesterol and control group. There was no difference among the groups in respect of TBARS, GPx and CAT levels. Conclusion:These results indicate that panax ginseng might be beneficial to reduce risk of hypercholesterolemia-induced oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Effects of ginseng on antioxidant enzymes in rats fed cholesterol rich diet

Uluişik¹,

Keskin²

2016

EurasianJVetSci

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“…The GSH levels and the GPx activity were strongly correlated with tissue benzo(a)pyrene and total PAHs' levels. Recently, Lin and Yang (2007) reported that in human hepatoma cells (HepG2), sub-lethal concentrations of benzo (a) pyrene caused a dose-dependent increase in GSH.…”

Section: Discussionmentioning

confidence: 99%

The mechanism of the antioxidant effect of smoked paprika from La Vera, Spain

Tormo

Campillo

Viña

et al. 2013

CyTA - Journal of Food

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This study aimed at further investigating the antioxidant effect of smoked paprika (Pimento´n La Vera, Spain) in healthy subjects. Crossover study included male students (age 20.6 + 1.7 years) who consumed smoked or non-smoked paprika (2 g/day, 20 times, over 30 days). After ingestion of smoked paprika, plasma malondialdehyde (MDA) levels were significantly (p 5 0.01) reduced versus basal and non-smoked paprika ingestion values. Reduced glutathione (GSH) levels were increased and oxidised glutathione (GSSG) levels reduced significantly (p 5 0.05) in paired subjects after consumption of smoked paprika. Smoked paprika significantly increased mRNA levels of glutathione peroxidase and cytochrome c oxydase versus non-smoked paprika ingestion. The low dose of polycyclic aromatic hydrocarbons (PAHs) contained in the smoked paprika could cause an activation of antioxidant defences as increasing activity of the GSH cycle and activating some antioxidant enzyme such as glutathione peroxidase and cytochrome c oxidase.Keywords: smoked paprika; reduced and oxidised glutathione; glutathione peroxidase; cytochrome c oxydase Este estudio investiga el efecto antioxidante del pimento´n ahumado (Pimento´n La Vera, Espan˜a) en sujetos sanos. Estudiantes varones (edad 20,6 + 1,7 an˜os) consumieron pimento´n ahumado o no ahumado (2 g/dı´a, 20 veces, durante 30 dı´as). Tras la ingestio´n del pimento´n ahumado los niveles plasma´ticos de malondialdehido (MDA) se redujeron (p 5 0,01) frente a los valores basales y a los obtenidos tras la ingestio´n de pimento´n no ahumado. Los niveles de glutatio´n reducido (GSH) aumentaron y los de glutatio´n oxidado (GSSG) disminuyeron (p 5 0,05) tras la ingestio´n de pimento´n ahumado. La ingestio´n de pimento´n ahumado incremento´significativamente los niveles de mRNA de glutatio´n peroxidasa y citocromo c oxidasa. Las pequen˜as dosis de hidrocarburos policı´clicos aroma´ticos (PHAs) contenidas en el pimento´n ahumado activarı´an las defensas antioxidantes al aumentar la actividad de sistemas antioxidantes como el ciclo GSH y la sı´ntesis de enzimas como glutatio´n peroxidasa y citocromo c oxidasa.

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“…Moreover, GPX could remove hydrogen peroxide generating in the tissues. Meanwhile, GSH is the most important biomolecule against chemically induced toxicity and can participate in the elimination of reactive intermediates by reducing hydroperoxides in the presence of GPX [27,28]. GSH also functions as a free radical scavenger and in the repair of free radical-induced biological damages [28].…”

Section: Discussionmentioning

confidence: 99%

The antioxidant activity and hypolipidemic activity of the total flavonoids from the fruit of Rosa laevigata Michx

Lu¹,

Xu²,

Yin³

et al. 2010

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In the present work, the antioxidant activity in vitro and hypolipidemic activity of the total flavonoids (TFs) from the Rosa laevigata Michx fruit was evaluated, and the antioxidant effect in vivo was also discussed. The TFs exhibited a high scavenging effect on 2, 2-diphenyl-1-picrylhydrazyl (·DPPH) with IC 50 values 0.01 mg/mL, and a stong reduce power in the test. Hyperlipemic mice were intragastric administrated with TFs (25, 50 mg/kg/day) for 4 weeks, and fenofibrate was used as the positive reference substance. After the experiment, the levels of TC (total cholesterol), TG (triglyceride), LDL-C (low density lipoprotein-cholesterol) of the mice administrated with high-dose of TFs were markedly declined by 45.02%, 33.86% and 73.68%, respectively, while HDL-C (high density lipoprotein-cholesterol) was significantly increased compared with model group. To investigate the hepatoprotective effect, histopathological assay, ALT (alanine aminotransferase), AST (aspartate aminotransefrase) and ALP (alkaline phosphatase) were also studied, and the results showed that TFs exhibited a favorable effect on liver protection, of which the levels of ALT, AST and ALP were elevated by 55.85%, 29.15% and 25.68%, respectively. Furthermore, the TFs could significantly decrease the MDA (malondialdehyde) level and improve the levels of CAT (Catalas), SOD (superoxide dismutase), GSH (reduced glutathione), and GPX (glutathione peroxidase) compared with hyperlipemia mice. Our results suggested that TFs has a high antioxidant activity and hypolipidemic activity, which can be used as a potential medicine for cardiovascular diseases.

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Benzo[a]pyrene-induced elevation of GSH level protects against oxidative stress and enhances xenobiotic detoxification in human HepG2 cells

Cited by 71 publications

References 62 publications

Effects of ginseng on antioxidant enzymes in rats fed cholesterol rich diet

Effects of ginseng on antioxidant enzymes in rats fed cholesterol rich diet

The mechanism of the antioxidant effect of smoked paprika from La Vera, Spain

The antioxidant activity and hypolipidemic activity of the total flavonoids from the fruit of Rosa laevigata Michx

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