Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development

Fang, Xiefan; Thornton, Cammi; Scheffler, Brian E.; Willett, Kristine L.

doi:10.1016/j.etap.2013.02.014

Cited by 104 publications

(77 citation statements)

References 80 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may be one of the mechanisms of RASSF1-mediated oncogenesis. Previous studies reported that abnormal expression of the RASSF1 gene is a critical step in oncogenesis and that it is a potential factor controlling cell division (White, 2012;Zhou and Zhu, 2012;Chan and Katan, 2013;Fang et al, 2013b). We found that when RASSF1 was expressed at high levels, the malignancy and re-currence rate of lacrimal gland carcinoma were significantly lower than those in low RASSF1 expression patients, further illustrating its importance during tumor occurrence.…”

Section: Discussionmentioning

confidence: 46%

Expression profile of tumor suppressor gene RASSF1 in lacrimal gland carcinoma

et al. 2015

View full text Add to dashboard Cite

ABSTRACT. We examined the expression pattern of the tumor suppressor gene RAS association domain family gene 1 (RASSF1) in lacrimal gland carcinoma and analyzed its relationship with the oncogenesis and progression of tumors. Sixty-two patients (30 males, 32 females, average age = 47 ± 3.5 years) admitted with lacrimal gland carcinoma to the Department of Ophthalmology of our hospital between January 2012 and January 2014 were enrolled in this study. Based on tumor malignancy, patients were classified into a malignant group (N = 25) and benign group (N = 37). Healthy lacrimal gland resections from trauma surgery (N = 35) were recruited as a healthy control group. Expression profiles of RASSF1 in all groups were quantified using reverse transcription-polymerase chain reaction and western blotting. Recurrence of lacrimal gland carcinoma was surveyed through postoperative follow-up. Expression levels of RASSF1 in samples from the malignant and benign groups were significantly lower than those in the healthy group (P < 0.05). Furthermore, the malignant group showed lower RASSF1 expression than the benign group (P < 0.05). Postoperative follow-up identified 22 cases of recurrence in the malignant group, with a recurrence rate of 88%, while 15 cases in the benign group had a C.H. Zeng et al. 6994©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6993-6998 (2015) recurrence rate of 40.5%. A direct relationship exists between RASSF1 expression levels and the malignancy grade of lacrimal gland carcinoma. Patients with lower RASSF1 expression showed a higher recurrence probability, indicating unfavorable prognosis. Therefore, measuring RASSF1 expression can be used as a diagnostic method for lacrimal gland carcinoma.

show abstract

Section: Discussionmentioning

confidence: 46%

Expression profile of tumor suppressor gene RASSF1 in lacrimal gland carcinoma

et al. 2015

View full text Add to dashboard Cite

show abstract

“…However, in fish for example, cases of developmental reprogramming by stress and glucocorticoids have been reported (Varsamos et al, 2006;Auperin and Geslin, 2008;Vijayan, 2012, 2013). Moreover, it is already known that the basics of the epigenetic regulation machinery are well conserved in fish and that the fish epigenome is susceptible to environmental influences (Aniagu et al, 2008;Li et al, 2009;Wang et al, 2009;Stroemqvist et al, 2010;Pierron et al, 2011;Fang et al, 2013). Nonetheless, a recent study found little similarity between epigenetic responses induced by several model chemicals in fish and mammals (Olsvik et al, 2014).…”

Section: Aops For Delayed Toxicity and Epigenetic Effects Of Chemicalsmentioning

confidence: 91%

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: I. Challenges and research needs in ecotoxicology

et al. 2015

View full text Add to dashboard Cite

To elucidate the effects of chemicals on populations of different species in the environment, efficient testing and modeling approaches are needed that consider multiple stressors and allow reliable extrapolation of responses across species. An adverse outcome pathway (AOP) is a concept that provides a framework for organizing knowledge about the progression of toxicity events across scales of biological organization that lead to adverse outcomes relevant for risk assessment. In this paper, we focus on exploring how the AOP concept can be used to guide research aimed at improving both our understanding of chronic toxicity, including delayed toxicity as well as epigenetic and transgenerational effects of chemicals, and our ability to predict adverse outcomes. A better understanding of the influence of subtle toxicity on individual and population fitness would support a broader integration of sublethal endpoints into risk assessment frameworks. Detailed mechanistic knowledge would facilitate the development of alternative testing methods as well as help prioritize higher tier toxicity testing. We argue that targeted development of AOPs supports both of these aspects by promoting the elucidation of molecular mechanisms and their contribution to relevant toxicity outcomes across biological scales. We further discuss information requirements and challenges in application of AOPs for chemical- and site-specific risk assessment and for extrapolation across species. We provide recommendations for potential extension of the AOP framework to incorporate information on exposure, toxicokinetics and situation-specific ecological contexts, and discuss common interfaces that can be employed to couple AOPs with computational modeling approaches and with evolutionary life history theory. The extended AOP framework can serve as a venue for integration of knowledge derived from various sources, including empirical data as well as molecular, quantitative and evolutionary-based models describing species responses to toxicants. This will allow a more efficient application of AOP knowledge for quantitative chemical- and site-specific risk assessment as well as for extrapolation across species in the future.

show abstract

“…This difference in development time means that medaka have an exposure duration which is longer than zebrafish. Zebrafish and Japanese medaka have shown reliable results in toxicity assessment using aqueous exposure (Fallahtafti et al 2012;Fang et al 2013;Hawliczek et al 2012;Huang et al 2012;Incardona et al 2011). In the case of hydrophobic compounds, direct waterborne exposure is not suitable and therefore alternative tests had to be developed.…”

Section: Introductionmentioning

confidence: 99%

Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays

Perrichon

Bihanic

Bustamante

et al. 2014

Environ Sci Pollut Res

View full text Add to dashboard Cite

Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10-day zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals testing. Second, a comparative study of toxicity responses from this exposure protocol was then performed using another OECD species, the Japanese medaka. The potential toxicity of both PAHs was assessed through lethal (e.g., survival, hatching success) and sublethal endpoints (e.g., abnormalities, PMR, and EROD) measured at different developmental stages, adapted to the embryonic development time of both species. Regarding effects observed for both species, a suitable artificial sediment composition for PAH toxicity testing was set at 92.5 % dry weight (dw) silica of 0.2-0.5-mm grain size, 5 % dw kaolin clay without organic matter for zebrafish, and 2.5 % dw blond peat in more only for Japanese medaka. PAH bioavailability and toxicity were highly dependent on the fraction of organic matter in sediment and of the K ow coefficients of the tested compounds. The biological responses observed were also dependent of the species under consideration. Japanese medaka embryos appeared more robust than zebrafish embryos for understanding the toxicity of PAHs following a sediment contact test, due to the longer exposure duration and lower sensitivity of sediment physical properties.

show abstract

Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development

Cited by 104 publications

References 80 publications

Expression profile of tumor suppressor gene RASSF1 in lacrimal gland carcinoma

Expression profile of tumor suppressor gene RASSF1 in lacrimal gland carcinoma

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: I. Challenges and research needs in ecotoxicology

Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays

Contact Info

Product

Resources

About