Benzo(a)pyrene-albumin adducts in humans exposed to polycyclic aromatic hydrocarbons in an industrial area of Poland.

Kure, Elin H.; Andreassen, Åshild; Øvrebø, Steinar; Grzybowska, Ewa; Fiala, Zdeněk; Stróżyk, M.; Chorazy, M; Haugen, Aage

doi:10.1136/oem.54.9.662

Cited by 23 publications

(17 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The finding of an increase in BPDE-Alb adducts in workers due to exposure to COE in present study is consistent with other investigations,3436 although Omland et al 37 and Kure et al 38 showed no statistical significance in the levels of B a P-albumin adducts between the exposed and control groups and no contribution of occupational exposure to COE to the formation of adducts. In addition, some results from other studies also showed that the BPDE protein adducts in workers exposed to COE were not affected by smoking,34 37 39 probably because the exposure to COE in coke oven workers is overwhelmingly high compared with exposure to smoking.…”

Section: Discussionsupporting

confidence: 92%

Association between plasma BPDE-Alb adduct concentrations and DNA damage of peripheral blood lymphocytes among coke oven workers

et al. 2007

View full text Add to dashboard Cite

Objectives: Coke oven emissions (COE) containing polycyclic aromatic hydrocarbons (PAHs) can induce both benzo [a]pyrene-r-7, t-8, t-9,c-10-tetrahydotetrol-albumin (BPDE-Alb) adducts and DNA damage. However, the relation between these biomarkers for early biological effects is not well documented in coke oven workers. Methods: In this study, the authors recruited 207 male workers exposed to COE and 102 controls not exposed to COE in the same steel plant in northern China. They measured BPDE-Alb adduct concentrations in plasma with reverse-phase high performance liquid chromatography and DNA damage in peripheral blood lymphocytes with alkaline comet assay. Results:The results showed that the median concentration of BPDE-Alb adducts in the exposed group (34.36 fmol/mg albumin) was significantly higher than that in the control group (21.90 fmol/mg albumin, p = 0.012). The mean Olive tail moment (Olive TM) of DNA damage in the exposed and control groups were 1.20 and 0.63, respectively (p = 0.000). Multivariate logistic regression analysis revealed that the odds ratio (OR) for BPDE-Alb adduct and Olive TM associated with the exposure were 1.72 (95% CI 1.06 to 2.81) and 1.96 (95% CI 1.20 to 3.19), respectively. These results show significant correlations between the concentrations of BPDE-Alb adduct and Olive TM levels in exposed group (r = 0.235, p = 0.001) but not in control group (r = 0.093, p = 0.353). Conclusion:The results suggest that occupational exposure to COE may induce both BPDE-Alb adducts and DNA damage in the lymphocytes of coke oven workers and that these two markers are useful for monitoring exposure to COE in the workplace.

show abstract

Section: Discussionsupporting

confidence: 92%

Association between plasma BPDE-Alb adduct concentrations and DNA damage of peripheral blood lymphocytes among coke oven workers

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Personal exposures to B[a]P in this study were 2 or 3 times higher than in the former study (6). Kure et al (11) analyzed benzo[a]pyrene diolepoxide (BPDE)-albumin adducts in coke oven workers. They found no difference between exposed and control groups.…”

mentioning

confidence: 74%

“…In all three studies that determined the effect of exposure to PAHs in coke oven workers (11), to nitroPAHs in bus garage workers (21), or to epichlorohydrin in factory workers (40), no effects of exposure on these protein adducts were observed.…”

Section: Discussionmentioning

confidence: 99%

“…Genetic polymorphisms of CYP2E1, GSTM1, and PONaffected the frequency of CAs using a challenge assay (41). No effects of GSTM1, NAT2, and/or CYPJAJ were observed with exposure to PAHs in coke ovens (6)(7)(8)(9)(10)(11)(12)(13)(14) and oil well fires (20), to organic solvents (34) or to pesticides (44,56).…”

Section: Discussionmentioning

confidence: 99%

“…(14). Coke oven and foundry workers certainly represent a group of subjects highly exposed to PAHs (6)(7)(8)(9)(10)(11)(12)(13). Using biomarkers of exposure such as DNA adducts (6,13) and 1-OHpyrene (9), significantly higher levels of PAHs were seen in these workers compared with levels in control groups.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Molecular Epidemiology Studies on Occupational and Environmental Exposure to Mutagens and Carcinogens, 1997-1999

Srám¹,

Binková²

2000

Environmental Health Perspectives

View full text Add to dashboard Cite

Molecular epidemiology is a new and evolving area of research, combining laboratory measurement of internal dose, biologically effective dose, biologic effects, and influence of individual susceptibility with epidemiologic methodologies. Biomarkers evaluated were selected according to basic scheme: biomarkers of exposure-metabolites in urine, DNA adducts, protein adducts, and Comet assay parameters; biomarkers of effect-chromosomal aberrations, sister chromatid exchanges, micronuclei, mutations in the hypoxanthine-guanine phosphoribosyltransferase gene, and the activation of oncogenes coding for p53 or p21 proteins as measured on protein levels; biomarkers of susceptibility-genetic polymorphisms of genes CYP1A1, GSTM1, GSTT1, NAT2. DNA adducts measured by 32P-postlabeling are the biomarker of choice for the evaluation of exposure to polycyclic aromatic hydrocarbons. Protein adducts are useful as a biomarker for exposure to tobacco smoke (4-aminobiphenyl) or to smaller molecules such as acrylonitrile or 1,3-butadiene. Of the biomarkers of effect, the most common are cytogenetic end points. Epidemiologic studies support the use of chromosomal breakage as a relevant biomarker of cancer risk. The use of the Comet assay and methods analyzing oxidative DNA damage needs reliable validation for human biomonitoring. Until now there have not been sufficient data to interpret the relationship between genotypes, biomarkers of exposure, and biomarkers of effect for assessing the risk of human exposure to mutagens and carcinogens. Key words: air pollution, benzene, biomarkers, coke oven, genotypes, occupational exposure, PAHs, pesticides, tobacco smoke. -Envwron Health Perspect 1 08(suppl 1):57-70 (2000). http.//ehpnetl. niehs. nih.gov/docs/2000/suppl-1/57-70sram/abstract. html A new and evolving area of research termed molecular epidemiology attempts to merge sophisticated and highly sensitive laboratory methods (many of them developed during the recent revolution in molecular biology) with analytical epidemiologic methods. Molecular epidemiology bridges basic research in molecular biology and studies of human cancer causation by combining laboratory measurement of internal dose, biologically effective dose, biologic effects, and the influence of individual susceptibility with epidemiologic methodologies (1). The most common view is that the approach represents a natural convergence of molecular biology and epidemiology (2).The number of biomarkers available for evaluating genetic and cancer risk in humans is quite large. Their utility-for human biomonitoring is suggested by the well-known paradigm of environmentally induced cancer, which represents end points for assessing the entire spectrum of human-genotoxicant interactions (3). These biomarkers begin with exposure and include absorption, metabolism, distribution, critical target interaction (i.e., DNA damage and repair), genetic changes, and finally, disease, which is the province of traditional epidemiology. The development of biomarkers has given rise to the field of ...

show abstract

The influence of cytochrome P450 1A1 and glutathione S-transferase M1 genotypes on biomarker levels in coke-oven workers

Brescia¹,

Celotti

Clonfero

et al. 1999

Archives of Toxicology

View full text Add to dashboard Cite

The present study has the aim of evaluating gene-environment interaction on the levels of different biomarkers in coke-oven workers exposed to PAH. In order to assess whether the levels of some biomarkers (PAH-DNA adducts, nitro-PAH adducts to Hb and MN frequency) could be modulated by the genetic metabolic polymorphisms for CYP1A1 and GSTM1, we analysed in 76 coke-oven workers and 18 controls the CYP1A1 (MspI and Ile/Val sites) and the GSTM1 genotypes by a PCR assay. In individuals with shared setup of CYP1A1 or GSTM1 genotypes, we analysed how the specified biomarkers correlated with total PAH exposure (urinary levels of 1-hydroxypyrene) both by a stratified analysis and logistic regression modelling. Statistically significant (P = 0.03 and P = 0.01) higher percentages of the more susceptible GSTM1- subjects compared to the GSTM1+ subjects and of the more susceptible CYP1A1 Ile/Val individuals compared to the CYP1A1 Ile/Ile individuals were detected for high levels of PAH-DNA adducts in the high exposure group (namely high levels of 1-OHP). A statistically significant association was observed between increased PAH-DNA adduct levels and the more susceptible GSTM1- genotype (P.O.R. = 4.18, P = 0.03) in a logistic regression modelling and a significant interaction between PAH exposure and GSTM1-genotype was found for PAH-DNA adducts. No effect of these metabolic genotypes was observed for MN frequency and nitro-PAH adducts to Hb. In conclusion, a gene-environment interaction between PAH exposure and two metabolic genotypes involved in activation (CYP1A1) and detoxification (GSTM1) of PAHs, respectively, has been identified.

show abstract

Benzo(a)pyrene-albumin adducts in humans exposed to polycyclic aromatic hydrocarbons in an industrial area of Poland.

Cited by 23 publications

References 25 publications

Association between plasma BPDE-Alb adduct concentrations and DNA damage of peripheral blood lymphocytes among coke oven workers

Association between plasma BPDE-Alb adduct concentrations and DNA damage of peripheral blood lymphocytes among coke oven workers

Molecular Epidemiology Studies on Occupational and Environmental Exposure to Mutagens and Carcinogens, 1997-1999

The influence of cytochrome P450 1A1 and glutathione S-transferase M1 genotypes on biomarker levels in coke-oven workers

Contact Info

Product

Resources

About