Benzo-(1, 2, 3)-thiadiazole-7-carbothioic acid s-methyl ester (BTH) promotes tuber wound healing of potato by elevation of phenylpropanoid metabolism

Jiang, Hong; Wang, Bin; Ma, Lan; Zheng, Xiaoyuan; Gong, Di; Xue, Huali; Bi, Yang; Wang, Yi; Zhang, Zhong; Prusky, Dov

doi:10.1016/j.postharvbio.2019.03.003

Cited by 65 publications

(31 citation statements)

References 44 publications

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“…Phenols and flavonoids are the end products of phenylpropanoid pathway, and their accumulation are closely associated with plant resistance and senescence [ 47 , 48 , 49 , 50 , 51 ]. Lignin enhances the mechanical strength of plant cell walls and prevents microbial invasion [ 29 ], and is involved in regulation of senescence [ 52 , 53 , 54 , 55 ]. In the present study, application of BTH promoted PAL, 4CL and C4H activities ( Figure 4 ), in consistence with the increased synthesis of phenols, flavonoids and lignin ( Figure 5 ) in pitaya fruit.…”

Section: Discussionmentioning

confidence: 99%

“…Of the synthesized secondary metabolites, some are antioxidants and antibiotic substances, which are important for maintenance of fruit quality and resistance to fungal infection [ 28 ]. It has been shown that some exogenous elicitors treatments enhance resistance against fungi and maintains fruit quality by activation of the phenylopropanoid pathway [ 8 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

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BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway

Ding

Zhu

Zheng

et al. 2021

Foods

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The plant resistance elicitor Benzo (1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) can enhance disease resistance of harvested fruit. Nonetheless, it is still unknown whether BTH plays a role in regulating fruit senescence. In this study, exogenous BTH treatment efficiently delayed the senescence of postharvest pitaya fruit with lower lipid peroxidation level. Furthermore, BTH-treated fruit exhibited lower hydrogen peroxide (H2O2) content, higher contents of reduced ascorbic acid (AsA) and reduced glutathione (GSH) levels and higher ratios of reduced to oxidized glutathione (GSH/GSSG) and ascorbic acid (AsA/DHA), as well as higher activities of ROS scavenging enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and glutathione reductase (GR) in comparison with control fruit. Moreover, BTH treatment enhanced the activities of phenylpropanoid pathway-related enzymes, including cinnamate-4-hydroxylase (C4H), phenylalanine ammonia-lyase (PAL) and 4-coumarate/coenzyme A ligase (4CL) and the levels of phenolics, flavonoids and lignin. In addition, BTH treatment upregulated the expression of HuSOD1/3/4, HuCAT2, HuAPX1/2 and HuPOD1/2/4 genes. These results suggested that application of BTH delayed the senescence of harvested pitaya fruit in relation to enhanced antioxidant system and phenylpropanoid pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway

Ding

Zhu

Zheng

et al. 2021

Foods

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“…However, the harvested potato tubers in fall easily suffer from cold stress and the healing is markedly slower ( Voss, 2016 ), therefore, it is necessary to determine the measures and related mechanism to accelerate the progress of wound healing. Our previous study indicated benzo-(1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH), an analog of salicylic acid (SA) and also the first artificially synthesized and commercialized elicitor registered as Bion ® or Actigard ® , stimulates defense responses via reactive oxygen species (ROS) production in diverse plants and promotes the wound healing of potato tubers by accelerating deposition of suberin and lignin at wound sites ( Jiang et al, 2019 ). Further research has demonstrated the elicited wound healing of potato tubers by BTH involves in ROS metabolism via an increase of the respiratory burst oxidase homolog (Rboh) activity and transcriptional levels, leading to the enhancement of ROS ( Jiang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

The Interaction Between StCDPK14 and StRbohB Contributes to Benzo-(1, 2, 3)-Thiadiazole-7-Carbothioic Acid S-Methyl Ester-Induced Wound Healing of Potato Tubers by Regulating Reactive Oxygen Species Generation

Jiang

et al. 2021

Front. Plant Sci.

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Reactive oxygen species (ROS) production is essential for both physiological processes and environmental stress in diverse plants. Previous studies have found that benzo-(1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH)-inducible ROS were associated with wound healing of potato tubers. Calcium-dependent protein kinases (CDPKs), the important calcium receptors, are known to play a crucial part in plant development and adaptation to abiotic stresses. However, whether CDPK-mediated ROS generation induced by BTH is involved in wound healing is elusive. In this study, we measured Solanum tuberosum CDPKs (StCDPKs) expression using real-time PCR, and it was found that the transcriptional levels of StCDPKs from BTH-treated tissues were significantly induced, among which StCDPK14 presented the most increased level. Subcellular localization results showed that StCDPK14 is located in the nucleus and membrane. The transgenic potato plants and tubers were developed using interference-expression of StCDPK14 by Agrobacterium tumefaciens–mediated transformation. The St respiratory burst oxidase homologs (StRbohs) expression showed a remarkable decrease in StCDPK14 transgenic tubers, notably, H2O2 content and suberin deposition were also significantly declined. To confirm the relationship between StCDPK14 and StRbohB, yeast-two-hybrid and bimolecular fluorescence complementation were used to examine the interaction, and it was shown that StCDPK14 interacted with the specific Ca2 + -binding motif (helix-loop-helix, called EF-hand) of StRbohB N-terminus. The above results unraveled that StCDPK14 functions in ROS generation via interacting with StRbohB during wound healing of potato tubers.

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“…In recent years, the analogues of these phytohormones modulating the corresponding pathways have been investigated by researchers [5,6]. Several chemical elicitors have been commercially used in greenhouse disease control, such as benzo (1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH), 2,6-dichloroisonicotinic acid (INA), β-aminobutyric acid (BABA), tiadinil (TDL), and pyraclostrobin [7][8][9][10][11]. Numerous chemical elicitors have been found to trigger direct or indirect plant-induced defense responses against herbivores in the laboratory, such as methyl jasmonate (MeJA), coronalon, laminarin, and so on [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

JA-Ile-Macrolactone 5b Induces Tea Plant (Camellia sinensis) Resistance to Both Herbivore Ectropis obliqua and Pathogen Colletotrichum camelliae

Lin

Dong

et al. 2020

IJMS

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Jasmonates (JAs), the group of lipid-derived hormones, were found to control the defense responses in a myriad of plants. Meaningfully, the macrolactones of 12-hydroxy jasmonate isoleucine (12OH-JA-Ile) were reported to induce the defensive response of wild tobacco. However, little to nothing has been known about the elicitation effect of JA-Ile-macrolactones on woody plants to harmful organisms, let alone its underlying mechanisms. Here, we first optimized the synthetic routine using mild toxic reagent isobutyl chloroformate instead of ethyl chloroformate for conjugation, and we used acetonitrile (MeCN) instead of ethyl alcohol for the better dissolution of p-toluenesulfonic acid (p-TsOH) to gain JA-Ile-macrolactones. JA-Ile-macrolactone 5b-treated tea plants significantly inhibited the larvae weight gain of Ectropis obliqua larvae and the lesions caused by Colletotrichum camelliae. Furthermore, the expression level of CsOPR3 was significantly upregulated in 5b-treated leaves. Meanwhile, 5b reduced the accumulation of eriodictyol 7-O-glucuronide (EDG) in tea plants, which was confirmed to promote the growth rate of E. obliqua larvae by artificial diet assay. In conclusion, our study proved that the exogenous application of 5b could induce the tea plant resistance both to herbivore E. obliqua and pathogen C. camelliae, and EDG was identified as one of the secondary metabolites that could influence the growth rate of E. obliqua, but it did not directly influence the infection of C. camelliae in vitro. Further research should be carried out to clarify the mechanism through which 5b induces tea plant resistance to C. camelliae.

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Benzo-(1, 2, 3)-thiadiazole-7-carbothioic acid s-methyl ester (BTH) promotes tuber wound healing of potato by elevation of phenylpropanoid metabolism

Cited by 65 publications

References 44 publications

BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway

BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway

The Interaction Between StCDPK14 and StRbohB Contributes to Benzo-(1, 2, 3)-Thiadiazole-7-Carbothioic Acid S-Methyl Ester-Induced Wound Healing of Potato Tubers by Regulating Reactive Oxygen Species Generation

JA-Ile-Macrolactone 5b Induces Tea Plant (Camellia sinensis) Resistance to Both Herbivore Ectropis obliqua and Pathogen Colletotrichum camelliae

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