Benzimidazole and imidazole fungicide analysis in grape and wine samples using a competitive enzyme-linked immunosorbent assay

Abstract

This manuscript reports a quantitative pesticide residue method analysis employing an enzyme-linked immunosorbent assay (ELISA) for detecting carbendazim, imazalil and thiabendazole pesticide residues in vegetable samples. The results have been compared with those obtained from a liquid chromatography system coupled to a triple quadrupole mass spectrometer (LC-ESI-QqQ-MS). A validation study was undertaken to establish this method's performance characteristics on the 3 pesticide residues in two commodities (table grapes and wine). The carbendazim, imazalil and thiabendazole ELISA showed an IC 50 reporting limit ranging from 0.5 to 13.8 mg kg À1 . The limit of quantification of the developed assay was 5 mg kg À1 for both grape and wine matrices. The optimised assay was compared with a reference procedure based on liquid chromatography using samples acquired from various supermarkets, and a good correlation between both methods was found.

Utilizing a Rapid Multi-Plug Filtration Cleanup Method for 72 Pesticide Residues in Grape Wines Followed by Detection with Gas Chromatography Tandem Mass Spectrometry

“…The analysis of pesticide residues in wine is challenging due to the complexity of the matrix, which contains alcohol, organic acids, sugars and polyphenols (e.g., anthocyanins, flavonols and tannins). Many effective preparation methods of wine samples have been reported, including liquid-liquid extraction (LLE) with different organic solvents [11,[18][19][20], solid-phase extraction (SPE) with reversed-phase C18 or polymeric sorbents [21][22][23][24][25][26][27], solid-phase microextraction (SPME) [22,28,29] and ultrasound-assisted emulsification microextraction (USAEME) [28,30], single drop liquid-liquid microextraction (LLME) [31][32][33][34], membrane-assisted solvent extraction [4] and dispersive liquid-liquid microextraction (DLLME) [35][36][37].…”

Rapid Determination of Carbendazim Residues in Mushrooms by Immunosorbent Assay1

“…Some sensitive and selective ELISAs for quantifing carbendazim in fruits and vegetables had been set up and applied [9][10] . In 2018, Guo, Linglinget al [11] had proposed a method based on the inhibition of plant esterase from pesticides and validated for the determination of carbendazim residues in aqueous samples. After optimization in pH, temperature, and detectiontime, a lower detection limit of 0.105 mu M was obtained in the linear range from 0.105 to 41.84 mu M. In 2015, Ana Ucl´es et al [12] had established an ELISA for detecting carbendazim, imazalil and thiabendazole residues in vegetable samples, and through 2h' testing, the 0.5 µg•kg -1~1 3.8 µg•kg -1 of IC 50 value of the calibration curve was obtained, after simple pretreatment, the method has a linearity range of 10 µg•kg -1 ~80 µg•kg.…”

Adsorption-Desorption Mechanism of Synthesized Benzimidazole Based Fungicide 2-(3’-Pyridyl) on Selected Soil Minerals

“…Fungicides pose great risk to human beings as a huge number of antagonistic effects have been observed (Santana-Rodríguez et al, 2010). Benzimidazole based fungicides are utilized in crops as anthelmintic agents and systemic fungicides to control both pre and post-harvest control (Uclés et al, 2015). Fungicides resistance has been found in several fields thus there is need for manufacturing new fungicides (Panebianco et al, 2015).…”