Benzene-, catechol-, hydroquinone- and phenol-induced cell transformation, gene mutations, chromosome aberrations, aneuploidy, sister chromatid exchanges and unscheduled DNA synthesis in Syrian hamster embryo cells

Tsutsui, Takeki; Hayashi, Nobuko; Maizumi, Heiji; Huff, James; Jc, Barrett

doi:10.1016/s0027-5107(96)00196-0

Cited by 158 publications

(93 citation statements)

References 37 publications

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“…SHE cell cultures were grown as described previously (2,3). Carbol camphor (CC) was prepared by mixing phenol (Wako Pure Chemical, Osaka) and DL-camphor (Kanto Chemical, Tokyo) at the molar ratio 3:1.…”

Section: Cells and Chemical Agentsmentioning

confidence: 99%

“…SHE cells have been utilized for a number of years to screen mutagens / carcinogens and to study the mechanism of carcinogenesis (2,3). Ten chemical agents are used as endodontic medicaments: carbol camphor, eugenol, guaiacol, and thymol are used as pulp sedatives and antiseptics for caries cavities or root canals; zinc oxide is used as a pulp capping agent and a root canal antiseptic; hydrogen peroxide is used as a root canal irrigant; m-cresol, formaldehyde, and glutaraldehyde are used as root canal antiseptics; and iodoform is used as a root canal filling agent.…”

Section: Introductionmentioning

confidence: 99%

“…Actual colony-forming efficiency of control cells was 13.0 ± 0.7% (S.D.). (2,3). The cells were harvested with 0.1% trypsin for chromosome preparation.…”

mentioning

confidence: 99%

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Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

et al. 2005

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Abstract. To assess the genotoxicity of 14 chemical agents used in dental practice, the ability of these agents to induce chromosome aberrations was examined using Syrian hamster embryo (SHE) cells. Statistically significant increases in the frequencies of chromosome aberrations were induced in SHE cells treated with 7 of 10 chemical agents used as endodontic medicaments, that is, carbol camphor, m-cresol, eugenol, guaiacol, zinc oxide, hydrogen peroxide, and formaldehyde. The other 3 chemical agents, that is, thymol, glutaraldehyde, and iodoform, did not increase the levels of chromosome aberrations. Of the 4 chemical agents that are used as an antiseptic on the oral mucosa, chromosome aberrations were induced by iodine, but not by the other 3 antiseptics, benzalkonium chloride, benzethonium chloride, and chlorhexidine. Among the 6 chemical agents exhibiting a negative response in the assay, only thymol induced chromosome aberrations in the presence of exogenous metabolic activation. Our results indicate that chemical agents having a positive response in the present study are potentially genotoxic to mammalian cells and need to be studied further in detail.

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Section: Cells and Chemical Agentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

et al. 2005

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“…Phenol has been shown to be a promoter in mouse skin exposure studies (59). Using Syrian hamster embryo (SHE) cells, phenol induced morphologic transformations, gene mutations at two loci (both ouabain-resistant and 6-thioguanine-resistant mutant frequencies were increased), chromosomal aberrations, sister chromatid exchanges, and unscheduled DNA synthesis (63). Obviously phenol is genotoxic.…”

Section: Phenolmentioning

confidence: 99%

Sawmill Chemicals and Carcinogenesis

Huff¹

2001

Environmental Health Perspectives

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“…Depending on the environmental concentration, phenol can lead to death to fi shes (Gupta et al 1982, Saha et al 1999. Multiple mechanism of action of phenol or some phenoli c compounds have been reported, including: drug antagonists (Roche and Bogé 2000), induction of genotoxic ity (Bolognesi et al 2006), carcinogenesis and mutagenesis (Tsutsui et al 1997, Yin et al 2006, endocrine disruption (Kumar and Mukherjee 1988), and metabolic disruption (Hori et al 2006). The evaluation of metabolic parameters has currently been used as biomarkers of environmental contamination by phenol (Hori et al 2006, Abdel-Hameid 2007, Avilez et al 2008, Varadarajan et al 2014, pesticides (Venkateswara Rao 2006, Aguiar et al 2004, Sancho et al 2009 and metals (Pretto et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Metabolic responses of channel catfish (Ictalurus punctatus) exposed to phenol and post-exposure recovery

Moraes

Rossi

Figueiredo

et al. 2016

An. Acad. Bras. Ciênc.

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Metabolic adjustments were studied in channel catfi sh Ictalurus punctatus exposed to 1.5 mg L -1 of phenol (10% LC50) for four days and recovered for seven days. Lower triacylglycerol (TGA) stores and increased muscle fat free acids (FFA) suggest fat catabolism in muscle. Remarkable liver FFA decrease (-31%) suggests liver fat catabolism as well. Increased muscular ammonia levels and ASAT (aspartate aminotransferase) and decreased plasma aminoacids suggest higher muscular amino acid uptake. Constant levels of glucose and increased liver glycogen stores, associated with lower amino acids in plasma, indicate gluconeogenesis from amino acids. This is supported by higher hepatic ALAT and ASAT. Higher hepatic LDH followed by lower plasma lactate may indicate that plasma lactate was also used as gluconeogenic substrate. Biochemical alterations were exacerbated during the post-exposure recovery period. Reduction in muscle and plasma protein content indicate proteolysis. A higher rate of liver fat catabolism was resulted from a remarkable decrease in hepatic TGA (-58%). Catabolic preference for lipids was observed in order to supply such elevated energy demand. This study is the fi rst insight about the metabolic profi le of I. punctatus to cope with phenol plus its ability to recover, bringing attention to the biological consequences of environmental contamination.

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Benzene-, catechol-, hydroquinone- and phenol-induced cell transformation, gene mutations, chromosome aberrations, aneuploidy, sister chromatid exchanges and unscheduled DNA synthesis in Syrian hamster embryo cells

Cited by 158 publications

References 37 publications

Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

Sawmill Chemicals and Carcinogenesis

Metabolic responses of channel catfish (Ictalurus punctatus) exposed to phenol and post-exposure recovery

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