Bennett J. Cohen

Fox, James G.; Anderson, Lynn C.; Loew, Franklin M.; Quimby, Fred W.

doi:10.1016/b978-012263951-7/50000-4

Cited by 1 publication

(3 citation statements)

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“…Atipamezole is an antagonist to medetomidine. Therefore, this can reverse rat very rapidly and also has few side effects [ 10 , 11 , 15 , 32 ]. When not neutralizing M/M/B, SpO 2 value does not fully rise (the data is not shown).…”

Section: Discussionmentioning

confidence: 99%

“…To control the spontaneous breathing of the rats, isoflurane was maintained at 3.0% concentration for males and at 2.5% for females following the connection of the anesthetic circuit for a period of 5 min. Subsequently, the concentration of isoflurane was decreased to 2.5% for males and 2.0% for females until the anesthesia was stopped [ 10 , 11 , 15 , 32 ]. Atipamezole (antisedan®, Nippon Zenyaku Kogyo Co., Ltd., Tokyo, Japan) is an antagonist to medetomidine and was intraperitoneally injected at a dose of 1.5 mg/kg b.w.…”

Section: Methodsmentioning

confidence: 99%

“…Anesthesia strongly influences the body of laboratory animals, and it can also greatly affect the experimental data, particularly the vital signs, such as heart rate, breath rate, SpO 2 and blood pressure. Therefore, appropriate and effective anesthetic methods are important for reliable laboratory experimentation [ 9 , 10 , 11 , 15 , 26 , 32 ]. Few studies have reported on the endotracheal intubation for small laboratory animals; mice [ 3 , 5 , 13 , 20 , 21 , 26 , 29 , 30 ], rats [ 4 , 12 , 17 , 24 , 27 ] and guinea pigs [ 5 ].…”

mentioning

confidence: 99%

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Visible, Safe and Certain Endotracheal Intubation Using Endoscope System and Inhalation Anesthesia for Rats

Konno

Shiotani

Itano

et al. 2014

The Journal of Veterinary Medical Science

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Anesthesia strongly influences laboratory animals, and it can also greatly affect the experimental data. Rats rank only second to mice in the number used in research fields, such as organ transplantation, regenerative medicine and imaging. Therefore, appropriate and effective anesthesia, including the protocol of the endotracheal intubation and inhalation anesthesia, is crucial. Hence, we evaluated these methods in this study. Twelve Wistar rats were intraperitoneally injected with M/M/B: 0.3/4/5, comprising of medetomidine, midazolam and butorphanol at a dose of 0.3 mg/kg + 4.0 mg/kg + 5.0 mg/kg body weight/rat, respectively. An endotracheal tube was then intubated into the trachea. After intubation, the rats were connected to the inhalation anesthesia circuit using isoflurane, and vital signs were measured until 30 min after connection. All intubations were successfully finished within 1 min, and the values of the vital signs were normal and stable. In addition, histopathological observation of the trachea and lungs showed no trauma. These results suggest that this visible endotracheal intubation method is simple, reliable, safe and favorable with regard to the rats’ welfare.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%