Benign prostatic hyperplasia complicated with T1DM can be alleviated by treadmill exercise—evidences revealed by the rat model

Abstract

BackgroundBoth benign prostatic hyperplasia (BPH) and Type-1 diabetes mellitus (T1DM) share similar epidemiologic features and are all associated with the insulin-like growth factor (IGF)-mediated hormonal imbalance. The purpose of this study is to understand whether exercise (EX) could alleviate DM and DM + BPH.MethodsSprague-Dawley rats were divided into eight groups: normal control, EX, BPH, BPH + EX, DM, DM + EX, BPH + DM, and BPH + DM + EX. T1DM was induced by intraperitoneal (ip) injection of streptozotocin (65 mg/kg) in Week 2, and BPH was induced by successive ip injections of Sustanon® (testosterone, 3.5 mg/head) plus estradiol (0.1 mg/head) from Week 3 to Week 9. Treadmill exercise training (20 m/min, 60 min per time) was performed three times per week for 6 weeks.ResultsIn BPH + EX, EX maintained at a constant body weight (BW); and suppressed stromal layer thickening, collagen deposition, blood glucose (BG), levels of testosterone (Ts), 5α-reductase(5αRd), dihydrotestosterone (DHT), androgen receptor (AR), serum hydrogen peroxide, TBARs, and interleukin-6 (IL-6). EX recovered testes size and substantially increased nitric oxide (NO) levels. In DM + EX group, EX decreased BW, PW, nuclear proliferation, inflammatory cell aggregation, collagen deposition, and BG. As contrast, EX upregulated insulin, IGF, Ts, NO, 5αRd, AR, and DHT, and substantially reduced PSA. In BPH + DM + EX, EX maintained BW at a subnormal level, slightly suppressed prostate stromal inflammation, collagen deposition, and BG, moderately restored sIn and IGF. Although failed to suppress Ts, EX highly upregulated 5αRd and suppressed DHT and AR, together with highly upregulated NO resulting in substantially reduced PSA.ConclusionEX, by remodeling androgen and NO expressions, can effectively alleviate BPH, DM, and BPH + DM.

Comparative RNA-sequencing analysis of the prostate in a mouse model of benign prostatic hyperplasia with bladder outlet obstruction

“…GSEA results showed that terms enriched for GO and KEGG were mainly associated with the regulation of endopeptidases and peptidases and complement and coagulation. GSVA showed that enriched GO and KEGG terms were mainly associated with cellular responses to insulin-like growth factor stimulation and autophagy, which is consistent with previous studies that have shown that BPH is associated with insulin-like growth factor-mediated hormone imbalance [ 77 ] and impaired autophagy [ 78 ].…”

Path of translational discovery of urological complications of obesity and diabetes

“…Impact of T1DM on the prostate. A number of studies report that induction of diabetes caused a significant reduction in serum testosterone levels and in prostatic weight in rats (19,54). The lobe response to diabetes varied (92).…”

Comparative RNA-sequencing analysis of the prostate in a mouse model of benign prostatic hyperplasia with bladder outlet obstruction

“…To further explore steroid hormonesinduced genomic differences in mice, we used GSEA and GSVA. GSEA results showed that terms enriched for GO and KEGG were mainly associated with the regulation of endopeptidases and peptidases and complement and coagulation GSVA showed that enriched GO and KEGG terms were mainly associated with cellular responses to insulin-like growth factor stimulation and autophagy, as previous studies have shown that BPH is associated with insulin-like growth factor-mediated hormone imbalance 53 and impaired autophagy 54 .…”