Benign and Malignant Renal Cells Are Differentially Inhibited during Prolonged Organ Preservation

Abstract

The worry of potential residual renal cancer cells in donor kidney after resection of small renal cancer impedes the extensive use of such controversial donor source. To explore the impacts of organ preservation process on the survival of renal cancer cells, we detected cell proliferation and viability of benign and malignant renal cell lines and clinical renal samples after treated with simulated organ preservation process. It was found that the viability and proliferation of malignant renal cells are inhibited much more than that of benign renal cells during prolonged organ preservation. The inhibition of proliferation in benign renal cells is fully reversible, while in malignant renal cancer cells is not fully reversible after a certain time. So potential residual renal cancer cells could be partly inhibited and eliminated by organ preservation process.

Long‐term outcome of kidney transplant by using restored kidney grafts after tumour ex vivo excision – a prospective study

“…However, this novel source remains arguable as the fear of tumour recurrence. Many pioneers have advocated the implementation of this novel source for kidney transplantation [19–22,25,41–44]. Cohn et al have campaigned a decade ago: ‘We encourage urologic oncologists to open discussions with transplant surgeons about considering transplantation of kidneys after ex vivo excision of small renal masses, from very selected donors (who prefer radical to partial nephrectomy) and into very selected (high‐risk) recipients’ [42].…”

“…Most interestingly, Yu et al have conducted a study aiming to test the viability of tumour cell after cold perfusion and preservation. It was found that the viability of tumour cell is much lower than normal renal tubular cell after cold perfusion and preservation [41]. Nevertheless, the tumour recurrence is always a risk after tumour excision and a strict protocol is mandatory for surveillance.…”