Benefits of TEMPOL on ram semen motility and in vitro fertility: a preliminary study

Ladu, Mara; Accardo, C; Pilichi, Susanna; Dattena, Maria; Chessa, F.; Chessa, Bernardo; Branca, Andrea; Cappai, P.

doi:10.1016/j.theriogenology.2004.10.005

Cited by 27 publications

(24 citation statements)

References 36 publications

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“…Improved motility and viability might be partially attributed to Tempolprotected lipid peroxidation [Acosta et al 2013]. In line with our results, previous studies in ram have shown that supplementation with Tempol increases sperm motility and fertility [Mara et al 2005]. However, in contrast to our results and those of Mara et al [2005], a recent study by Mata-Campuzano et al [2012] showed that antioxidants like Tempol and N-acetyl-cysteine (NAC) decrease sperm motility, while in accordance with our results, Tempol reduces ROS, DNA fragmentation, and lipid peroxidation due to cryopreservation induced oxidative stress.…”

Section: Discussionsupporting

confidence: 92%

“…In line with our results, previous studies in ram have shown that supplementation with Tempol increases sperm motility and fertility [Mara et al 2005]. However, in contrast to our results and those of Mara et al [2005], a recent study by Mata-Campuzano et al [2012] showed that antioxidants like Tempol and N-acetyl-cysteine (NAC) decrease sperm motility, while in accordance with our results, Tempol reduces ROS, DNA fragmentation, and lipid peroxidation due to cryopreservation induced oxidative stress. These authors and other studies suggest that the excessive reduction of ROS level by an antioxidant to below physiological level may inhibit sperm motility [Aitken and Curry 2011;Mata-Campuzano et al 2012].…”

Section: Discussionsupporting

confidence: 89%

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Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function

Bateni¹,

Azadi²,

Tavalaee³

et al. 2014

Systems Biology in Reproductive Medicine

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Despite extensive research carried out for optimization and commercialization of sperm cryopreservation media, percentage of motility and viability remain low following cryopreservation. These adverse effects have been partially ascribed to reactive oxygen species (ROS) production during cryopreservation. Therefore, we proposed that addition of a cell permeable antioxidant like Tempol, with superoxide dismutase (SOD) mimetic action, may overcome these effects in an optimized commercially available cryo-protective medium. Therefore, semen samples were cryopreserved in the presence or absence of Tempol. A concentration of 5 μM Tempol was defined as optimal since it significantly improved motility and viability post thawing and reduced DNA fragmented sperm. In addition, percentage of ROS positive sperm was reduced. These effects of Tempol can be attributed to cell permeability characteristic and ability to reduce superoxide production both at intra- and extra-cellular levels. Tempol may hold the potential for clinical applications.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function

Bateni¹,

Azadi²,

Tavalaee³

et al. 2014

Systems Biology in Reproductive Medicine

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“…For example, Maxwell and Stojanov (1996) found that pregnancy rates, after intrauterine insemination, were greater when semen was stored for 7 and 14 days that was supplemented with superoxide dismutase and catalase (41% vs. 16%). More recently, Mara et al (2005) found an improvement in fertility and blastocyst rate development in an in vitro experiment, when sperm were stored at 15 • C in presence of the antioxidant TEMPOL, and Casao et al (2010) reported an increase in the cleavage rate after treating sperm with small amounts of melatonin. In addition to the effects of antioxidants, there was an overall greater quality in the samples of the present study that were maintained at 15 • C. Although there are many reports on the quality of ram semen stored either at 15 or 5 • C (Salamon and Maxwell, 2000), only a few of these previous studies directly compared both temperatures under similar conditions (Colas et al, 1968;Hollinshead et al, 2004;O'Hara et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that many factors affect the sperm physiology during storage, and this scenario offers an important opportunity to test protective compounds such as antioxidants. With previous research, it has been suggested that adding antioxidants -either enzymatic (Maxwell and Stojanov, 1996) or non-enzymatic (Bucak et al, 2012;Mara et al, 2005) -could benefit the quality and fertility of ram semen after liquid storage at different temperatures. Therefore, a study was previously performed on the effects of the antioxidants Trolox and reduced glutathione (GSH) during cooled storage at 5 • C and 15 • C. Trolox, an analog of vitamin E, has been successfully tested for controlling lipid peroxidation in ram sperm during cryopreservation (Maia et al, 2010), and thiols have been proved to be excellent radical scavengers and blockers of lipid peroxidation (Deneke, 2000).…”

Section: Introductionmentioning

confidence: 99%

Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time

Mata-Campuzano

Álvarez‐Rodríguez

Tamayo-Canul

et al. 2014

Animal Reproduction Science

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Antioxidants have a potential to improve the quality and fertility of refrigerated-stored ram semen. Reduced glutathione (GSH) and Trolox (0.2, 1 and 5mM) were evaluated in ram semen preserved at 15 and 5°C up to 48 and 96h, respectively. Extenders were also evaluated (15°C: Tris-citrate-fructose, TCF, without lipids, and TES-Tris-fructose 10% egg yolk, TTF-EY; 5°C: TTF-EY and 3.5% soybean lecithin, TTF-SL; INRA96 at both temperatures). Storage at 5°C resulted in poorer quality than 15°C up to 48h, while allowing acceptable quality at 96h. Antioxidants had few effects on sperm quality, with use of Trolox resulting in reduced motility and viability in TCF. Storage at 15°C in the TCF extender resulted in decreased motility, viability and mitochondrial activity compared with use of TTF-EY. Sperm quality when storage was at 5°C was similar, but storage in TTF-SL resulted in decreased motility and mitochondrial activity. Acrosomal status was only slightly affected by extender and antioxidant. Mitochondrial activity was improved by antioxidants in TTF-SL, and GSH at 5mM when semen was stored at 5°C in TTF-EY. A preliminary artificial insemination trial indicated that supplementation with GSH has the potential for improving lambing (P<0.1). In conclusion, use of antioxidants resulted in lesser effects than extender composition or storage time on quality of ram semen. Use of Trolox negatively impacted sperm quality and GSH had some positive impacts. The use of soybean lecithin requires further research to assess its impact on mitochondria.

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“…El Tempo y Tempol han sido utilizados con la finalidad de prevenir la pérdida de motilidad y viabilidad espermática durante la refrigeración de los espermatozoides de ovino (Mara et al, 2005), equino (Ball et al, 2001), y pavo (Donogue y Donogue, 1997), así como durante el congelamiento de espermatozoides de ovino (Santiani, 2003) y bovino (Foote et al, 2002). Se ha encontrado que la adición de Tempo o Tempol a un medio en base a Tris en la criopreservación de semen bovino no afectó la motilidad espermática, y cuando se usó leche descremada como dilutor, la motilidad disminuyó significativamente (Foote et al, 2002), demostrando que los efectos del empleo de estos antioxidantes dependen del dilutor en que se encuentren.…”

Section: Introductionunclassified

Efecto De Dos Antioxidantes (Tempo Y Tempol) en La Criopreservación De Semen Ovino Empleando Un Dilutor en Base a Tris.

Santiani

Sandoval

et al. 2012

Rev. investig. vet. Perú

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RESUMENSe emplearon 32 muestras de semen procedentes de cuatro ovinos a fin de ser criopreservadas con la adición dos antioxidantes: Tempo (2,2,6,6 tetrametil-1-piperidiniloxil) y Tempol (4-hidroxi 2,2,6,6 tetrametil-1-piperidiniloxil), en concentraciones de 0.5, 1.0 y 2.5 mM, para evaluar el efecto postdescongelamiento que pudieran tener sobre la motilidad progresiva, la viabilidad e integridad acrosomal y la capacitación espermática prematura. En cada concentración de los dos antioxidantes y del grupo control se utilizó un dilutor en base a Tris, realizándose ocho repeticiones, cada una con cuatro muestras de semen. Los resultados obtenidos demuestran que la adición de Tempo a una concentración de 0.5 mM mejora significativamente la calidad del semen criopreservado en comparación con el grupo control (p<0.05), incrementado los porcentajes de motilidad progresiva (79 vs. 67%), viabilidad e integridad acrosomal (70 vs. 58%) y reduciendo la capacitación espermática prematura (9 vs.15%). Por otro lado, la adición de Tempol disminuyó la calidad seminal postdescongelamiento en comparación con el control. En conclusión, la adición de Tempo 0.5 mM en un dilutor en base a Tris, al finalizar la fase de enfriamiento, podría constituir una estrategia para mejorar la calidad de semen ovino criopreservado.Palabras clave: antioxidante, Tempo, Tempol, criopreservación, semen, ovino, Tris ABSTRACT Thirty-two semen samples from four rams were frozed using two antioxidants, Tempo (2,2,6,6 tetramethyl-1-piperidinyloxyl) and Tempol (4-hidroxi 2,2,6,6 tetramethyl-1-piperidinyloxyl), each one in concentrations of 0.5, 1.0, and 2.5 mM, to evaluate the effects on post-thawing motility, viability, acrosomal integrity, and earlier sperm capacitation. Eight replications for each antioxidant concentration and control group,

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Benefits of TEMPOL on ram semen motility and in vitro fertility: a preliminary study

Cited by 27 publications

References 36 publications

Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function

Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function

Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time

Efecto De Dos Antioxidantes (Tempo Y Tempol) en La Criopreservación De Semen Ovino Empleando Un Dilutor en Base a Tris.

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