Benefits of sea buckthorn (Hippophae rhamnoides) pulp oil‐based mouthwash on oral health

Abstract

Aim The purpose of this study was to conduct phytochemical analysis of sea buckthorn pulp oil and to evaluate the antimicrobial, anti‐biofilm and antioxidant activities of its mouthwash form. Methods and Results Fatty acid composition of the sea buckthorn pulp oil was determined by GC‐MS analysis, which revealed that, mono‐unsaturated fatty acid, palmitoleic acid and saturated fatty acid, palmitic acid, were the major constituents. The antimicrobial and the anti‐biofilm capacities of sea buckthorn pulp oil mouthwash form were evaluated against Streptococcus gordonii, Porphyromonas gingivalis, Actinomyces viscosus and Candida albicans, according to the European Norms, and the Biofilm Ring Test®, respectively. These activities were then compared with those of chlorhexidine and herbal mouthwashes. The sea buckthorn‐based mouthwash was bactericidal against S. gordonii and P. gingivalis, bacteriostatic against A. viscosus and showed no antifungal effect. Regardless of the strains used, complete inhibition of biofilm formation was achieved. The antioxidant activity of this experimental mouthwash was also assessed by DPPH and NBT assays. Conclusion Sea buckthorn mouthwash showed anti‐biofilm activities against select single and multiple oral bacterial species. Significance and Impact of the Study In this study, a mouthwash derived from sea buckthorn (Hippophae rhamnoides) pulp oil has been experimented, for the first time, in order to overcome the problem of a large number of available synthetic mouthwashes which have side effects on teeth, gums and mucous membranes. This mouthwash seemed to be a suitable alternative for a preventive agent for periodontal inflammation.

Sea buckthorn Hippophae rhamnoides a non-conventional source of edible oil

“…The present work indicated that the physicochemical values of the oil are not well within value reported in the literature but it is due to the state of i.e. crude and change of the climate from the others, but the oil does not seem the change its property and utilization as oil and medicine in recommended diseases [25].…”

“…The chart speed was adjusted to 10 mm/min. The peaks recorded at shimadzu recorder C-R4A chromatopac, were identified by comparison of their retention times with those of standard methyl esters analyzed under the same conditions [25]. The area of each peak was calculated by the formula; Area of peak = 1/2 x base perpendicular.…”

Advanced Research on the Antioxidant Activity and Mechanism of Polyphenols from Hippophae Species—A Review

“…Cosmetic products made of Hippophae plants include shampoo, skin cream and bath soak [102]. Smida [105] evaluated the antioxidant activities of sea buckthorn-based mouthwash; the free radical-scavenging activity of sea buckthorn was assessed by DPPH and superoxide anion scavenging (NBT) assays to elucidate the role of sea buckthorn in reducing oxidative stress and damage linked to periodontitis. The results suggested that the superoxide radical-scavenging activities of the sea buckthorn pulp oil-based mouthwash increased markedly with increasing concentrations, suggesting that the mouthwash is a potent scavenger of superoxide radicals.…”

Potential of sea buckthorn-based ingredients for the food and feed industry – a review

“…Also, Hou et al 2017proved that treatment with SB oil could suppress the development of atopic dermatitis-like lesions in mice, possibly proving the regenerating capacity of SB fruit oil. Smida et al (2019) have interestingly proved the efficacy of SB pulp oil as a mouthwash product. They found that a preparation containing SB pulp oil could have a bactericidal and anti-biofilm activities against oral bacteria species, although antifungal activities were not proved.…”