Benefits of PEGylation in the early post-transplant period of intraportal islet transplantation as assessed by magnetic resonance imaging of labeled islets

Jin, Sang‐Man; Oh, Seung-Hoon; Oh, Bong Ryoul; Suh, Sunghwan; Bae, Ji Cheol; Lee, Jun Young; Lee, Myung-Shik; Lee, Moon‐Kyu; Kim, Kwang-Won; Kim, Jae Hyun

doi:10.4161/isl.27827

Cited by 6 publications

(2 citation statements)

References 25 publications

(42 reference statements)

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“…Second, the procedure does not require surface modification of transplanted islets, which usually causes loss of therapeutic islet mass due to structural destruction of isolated islets. This is in contrast to other approaches, such as encapsulation of islets (30), islet surface camouflage (31,32), and coating the islet surface with endothelial cells (33) or endothelial progenitor cells (7). Third, our histological analysis of the recipient liver revealed small, elliptical BM-spheroids in more peripheral areas of the liver than the areas containing islet grafts.…”

Section: Discussionmentioning

confidence: 58%

Highly Angiogenic, Nonthrombogenic Bone Marrow Mononuclear Cell–Derived Spheroids in Intraportal Islet Transplantation

Jin

Hwang

et al. 2018

Diabetes

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Highly angiogenic bone marrow mononuclear cell-derived spheroids (BM-spheroids), formed by selective proliferation of the CD31CD14CD34 monocyte subset via three-dimensional (3D) culture, have had robust angiogenetic capacity in rodent syngeneic renal subcapsular islet transplantation. We wondered whether the efficacy of BM-spheroids could be demonstrated in clinically relevant intraportal islet transplantation models without increasing the risk of portal thrombosis. The thrombogenic potential of intraportally infused BM-spheroids was compared with that of mesenchymal stem cells (MSCs) and MSC-derived spheroids (MSC-spheroids). The angiogenic efficacy and persistence in portal sinusoids of BM-spheroids were examined in rodent syngeneic and primate allogeneic intraportal islet transplantation models. In contrast to MSCs and MSC-spheroids, intraportal infusion of BM-spheroids did not evoke portal thrombosis. BM-spheroids had robust angiogenetic capacity in both the rodent and primate intraportal islet transplantation models and improved posttransplant glycemic outcomes. MRI and intravital microscopy findings revealed the persistence of intraportally infused BM-spheroids in portal sinusoids. Intraportal cotransplantation of allogeneic islets with autologous BM-spheroids in nonhuman primates further confirmed the clinical feasibility of this approach. In conclusion, cotransplantation of BM-spheroids enhances intraportal islet transplantation outcome without portal thrombosis in mice and nonhuman primates. Generating BM-spheroids by 3D culture prevented the rapid migration and disappearance of intraportally infused therapeutic cells.

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Section: Discussionmentioning

confidence: 58%

Highly Angiogenic, Nonthrombogenic Bone Marrow Mononuclear Cell–Derived Spheroids in Intraportal Islet Transplantation

Jin

Hwang

et al. 2018

Diabetes

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“…The authors showed the ability to maintain survival and graft functions for up to a month. Their T1D rat model showed improved glycaemia although the model exhibited less severe diabetes development and diabetes symptoms compared with our mouse model [ 73 ]. In another study by Loganathan et al, and similar to a study by Sang-Man et al, the authors maintained diabetic mice for a month.…”

Section: Resultsmentioning

confidence: 99%

Taurine Grafted Micro-Implants Improved Functions without Direct Dependency between Interleukin-6 and the Bile Acid Lithocholic Acid in Plasma

et al. 2022

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A recent study showed an association between diabetes development and the bile acid lithocholic acid (LCA), while another study demonstrated positive biological effects of the conjugated bile acid, taurocholic acid (TCA), on pancreatic cells. Thus, this study aimed to encapsulate TCA with primary islets (graft) and study the biological effects of the graft, post-transplantation, in diabetic mice, including effects on LCA concentrations. Sixteen mature adult mice were made diabetic and randomly divided into two equal groups, control and test (transplanted encapsulated islets without or with TCA). Graft pharmaceutical features pre-transplantation, and biological effects including on LCA concentrations post-transplantation, were measured. TCA-microcapsules had an oval shape and similar size compared with the control. The treatment group survived longer, showed improved glucose and interleukin-6 concentrations, and lower LCA concentrations in plasma, large intestine, faeces, liver and spleen, compared with control. Results suggest that TCA incorporation with islets encapsulated graft exerted beneficial effects, but there was no direct and significant dependency between concentrations of interleukin-6 and LCA.

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Nanobased scientific and technological solutions for the management of diabetes mellitus

Picu

Petcu

Chifiriuc

2019

Biomedical Applications of Nanoparticles

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Benefits of PEGylation in the early post-transplant period of intraportal islet transplantation as assessed by magnetic resonance imaging of labeled islets

Cited by 6 publications

References 25 publications

Highly Angiogenic, Nonthrombogenic Bone Marrow Mononuclear Cell–Derived Spheroids in Intraportal Islet Transplantation

Highly Angiogenic, Nonthrombogenic Bone Marrow Mononuclear Cell–Derived Spheroids in Intraportal Islet Transplantation

Taurine Grafted Micro-Implants Improved Functions without Direct Dependency between Interleukin-6 and the Bile Acid Lithocholic Acid in Plasma

Nanobased scientific and technological solutions for the management of diabetes mellitus

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