Benefits of Zataria multiflora Boiss in Experimental Model of Mouse Inflammatory Bowel Disease

Abstract

Inflammatory bowel disease (IBD) is a chronic condition of the intestine with unknown etiology involving multiple immune, genetic and environmental factors. We were interested to examine the effect of total extract from Zataria multiflora Boiss, a folk medicinal plant on prevention and treatment of experimental IBD. Z. multiflora was administered (400, 600, 900 p.p.m.) through drinking water to IBD mice induced by intrarectal administration of acetic acid. Prednisolone was used as the standard drug for comparison. Biochemical, macroscopic and microscopic examinations of colon were performed. Biochemical evaluation of inflamed colon was done using assay of myeloperoxidase (MPO) activity and thiobarbituric acid reactive substances (TBARS) concentration as indicators of free radical activity and cell lipid peroxidation. The activity of MPO and lipid peroxidation products (TBARS) increased in acetic acid-treated groups while recovered by pretreatment of animals with Z. multiflora (400–900 p.p.m.) and prednisolone. Z. multiflora (600 and 900 p.p.m.) and prednisolone-treated groups showed significantly lower score values of macroscopic and microscopic characters when compared with the acetic acid-treated group. The beneficial effect of Z. multiflora (900 p.p.m.) was comparable with that of prednisolone. The antioxidant, antimicrobial and anti-inflammatory potentials of Z. multiflora might be the mechanisms by which this herbal extract protects animals against experimentally induced IBD. Proper clinical investigation should be carried out to confirm the activity in human.

The Possible Role of Mesenchymal Stem Cells Therapy in the Repair of Experimentally Induced Colitis in Male Albino Rats

“…This could be referred to the effect of the aggressor agent (acetic acid) inducing colonic injury resulting in epithelial mucosal damage and subsequent inflammation. The inflammation caused by acetic acid attributed to entry of soluble protonated form of the acid into the epithelium leading to massive intracellular acidification resulting in massive epithelial damage (24). Inflammation is a protective response intended to eliminate the initial cause of cell injury as well as the necrotic cells and tissues resulting from the original insult.…”

The zinc-finger protein KCMF1 is overexpressed during pancreatic cancer development and downregulation of KCMF1 inhibits pancreatic cancer development in mice

“…Transgenic TGF-a mice (Sandgren et al, 1993;Wagner et al, 2001), LSL-kRas G12D mice (Hingorani et al, 2003) and p48-cre mouse (Nakhai et al, 2007) are described. LSL-kRrasG12D mice were crossed to p48-Cre animals to generate mice with pancreas-specific (p48-kRas) and activate oncogenic kRas (Khasawneh et al, 2009).…”

Potential ofMoringa oleiferaroot andCitrus sinensisfruit rind extracts in the treatment of ulcerative colitis in mice

“…On the 8th day colitis was induced by intrarectal administration of 150 µl of 5% acetic acid (pH 2.5), 3 cm from the anal margin. Extract treatment was continued up to 10th day (Nakhai et al, 2006;Sonawane et al, 2011).…”