Benefits of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> Probiotic in Experimental Periodontitis

Oliveira, Luana Farias de; Salvador, Sérgio Luiz de Souza; Silva, Pedro Henrique Félix; Furlaneto, Flávia Aparecida Chaves; Figueiredo, Luciene Cristina; Casarin, Renato Corrêa Viana; Ervolino, Edílson; Palioto, Daniela Bazan; Souza, Sérgio Luı́s Scombatti de; Taba, Mário; Novaes, Arthur B.; Messora, Michel Reis

doi:10.1902/jop.2016.160217

Cited by 85 publications

(168 citation statements)

References 47 publications

(91 reference statements)

Supporting

Mentioning

128

Contrasting

Unclassified

Order By: Relevance

“…In a preclinical study, Oliveira et al. () demonstrated that B. lactis HN019 reduces the amount of periodontopathogens on biofilm. The possible coaggregation of Bifidobacterium with Fusobacterium nucleatum can reduce the amount of binding sites for periodontopathogens on biofilm (Haukioja et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Only two pre‐clinical studies evaluated the effect of bacteria of the genus Bifidobacterium on periodontal disease (Oliveira et al., ; Ricoldi et al., ). Oliveira et al.…”

Section: Introductionmentioning

confidence: 99%

“…Oliveira et al. () demonstrated that the topical use of Bifidobacterium animalis subsp. lactis HN019 promotes a protective effect against alveolar bone loss and connective tissue attachment loss attributable to experimental periodontitis in rats.…”

Section: Introductionmentioning

confidence: 99%

“…Only two pre-clinical studies evaluated the effect of bacteria of the genus Bifidobacterium on periodontal disease Ricoldi et al, 2017). Oliveira et al (2017) demonstrated that the topical use of Bifidobacterium animalis subsp. lactis HN019 promotes a protective effect against alveolar bone loss and connective tissue attachment loss attributable to experimental periodontitis in rats.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Effects of Bifidobacterium probiotic on the treatment of chronic periodontitis: A randomized clinical trial

Invernici

Salvador

Silva

et al. 2018

J Clinic Periodontology

Self Cite

172

231

View full text Add to dashboard Cite

AimThis randomized placebo‐controlled clinical trial evaluated the effect of Bifidobacterium animalis subsp. lactis (B. lactis) HN019‐containing probiotic lozenges as adjuvant to scaling and root planing (SRP) in patients with generalized chronic periodontitis.Materials and MethodsForty‐one chronic periodontitis patients were recruited and monitored clinically, immunologically, and microbiologically at baseline (before SRP) and 30 and 90 days after SRP. All patients were randomly assigned to a Test (SRP + Probiotic, n = 20) or Control (SRP + Placebo, n = 21) group. The probiotic lozenges were used twice a day for 30 days. The data were statistically analysed.ResultsThe Test group presented a decrease in probing pocket depth and a clinical attachment gain significantly higher than those of the Control group at 90 days. The Test group also demonstrated significantly fewer periodontal pathogens of red and orange complexes, as well as lower proinflammatory cytokine levels when compared to the Control group. Only the Test group showed an increase in the number of B. lactis HN019 DNA copies on subgingival biofilm at 30 and 90 days.ConclusionThe use of B. lactis HN019 as an adjunct to SRP promotes additional clinical, microbiological, and immunological benefits in the treatment of chronic periodontitis (NCT03408548).

show abstract

Section: Discussionmentioning

confidence: 99%

“…Only two pre‐clinical studies evaluated the effect of bacteria of the genus Bifidobacterium on periodontal disease (Oliveira et al., ; Ricoldi et al., ). Oliveira et al.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Effects of Bifidobacterium probiotic on the treatment of chronic periodontitis: A randomized clinical trial

Invernici

Salvador

Silva

et al. 2018

J Clinic Periodontology

Self Cite

172

231

View full text Add to dashboard Cite

show abstract

“…lactis , named HN019, on the development of experimental periodontitis (EP) in rats. It was observed that the treatment promoted a protective effect against periodontal breakdown, modifying immunoinflammatory and microbiological parameters [17]. To the best of our knowledge, there are no studies evaluating the effects of B .…”

Section: Introductionmentioning

confidence: 99%

Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats

Ricoldi¹,

Furlaneto²,

Oliveira³

et al. 2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Lactobacillus probiotics have been investigated in periodontitis. However, the effects of the genus Bifidobacterium on periodontitis are hardly known. This study evaluated the effects of the probiotic (PROB) Bifidobacterium animalis subsp. lactis (B. lactis) HN019 as an adjunct to scaling and root planing (SRP) in rats with experimental periodontitis (EP). At baseline, 32 rats were assigned to 4 groups: C (control), PROB, EP-SRP and EP-SRP-PROB. In groups EP-SRP and EP-SRP-PROB, the mandibular first molars of the animals received a ligature. At day 14, the ligatures were removed and SRP was performed. Animals of groups PROB and EP-SRP-PROB were orally administered with 10 mL/day of 109 colony forming units of B. lactis HN019 for 15 days, starting at day 14. Animals were euthanized at day 29. Histomorphometric, microtomographic and immunohistochemical analyses were performed. Microbiological effects of B. lactis on biofilm were also evaluated. Data were statistically analyzed (ANOVA, Tukey; Kruskal-Wallis, Dunn’s; Two-tailed t-test; p<0.05). Group EP-SRP-PROB presented reduced alveolar bone resorption and attachment loss when compared with Group EP-SRP (p<0.05). Group EP-SRP-PROB showed significantly fewer osteoclasts, increased expression of anti-inflammatory cytokines and reduced expression of proinflammatory cytokines compared with Group EP-SRP (p<0.05). B. lactis promoted a higher ratio between aerobic and anaerobic bacteria in biofilm samples (p<0.05). B. lactis HN019 may have a role in the treatment of EP in rats, as an adjunct to SRP.

show abstract

Chronological analysis of periodontal bone loss in experimental periodontitis in mice

Hernández Martínez,

Felix Silva,

Salvador

et al. 2023

Clinical & Exp Dental Res

Self Cite

View full text Add to dashboard Cite

ObjectivesPeriodontal disease is understood to be a result of dysbiotic interactions between the host and the biofilm, causing a unique reaction for each individual, which in turn characterizes their susceptibility. The objective of this study was to chronologically evaluate periodontal tissue destruction induced by systemic bacterial challenge in known susceptible (BALB/c) and resistant (C57BL/6) mouse lineages.Material and MethodsAnimals, 6–8 weeks old, were allocated into three experimental groups: Negative control (C), Gavage with sterile carboxymethyl cellulose 2%—without bacteria (Sham), and Gavage with carboxymethyl cellulose 2% + Porphyromonas gingivalis (Pg‐W83). Before infection, all animals received antibiotic treatment (sulfamethoxazole/trimethoprim, 400/80 mg/5 mL) for 7 days, followed by 3 days of rest. Microbial challenge was performed 3 times per week for 1, 2, or 3 weeks. After that, the animals were kept until the completion of 42 days of experiments, when they were euthanized. The alveolar bone microarchitecture was assessed by computed microtomography.ResultsBoth C57BL/6 and BALB/c mice exhibited significant bone volume loss and lower trabecular thickness as well as greater bone porosity compared to the (C) and (Sham) groups after 1 week of microbial challenge (p < .001). When comparing only the gavage groups regarding disease implantation, time and lineage, it was possible to observe that within 1 week of induction the disease was more established in BALB/c than in C57BL/6 (p < .05).ConclusionsOur results reflected that after 1 week of microbial challenge, there was evidence of alveolar bone loss for both lineages, with the loss observed in BALB/c mice being more pronounced.

show abstract

Benefits of Bifidobacterium animalis subsp. lactis Probiotic in Experimental Periodontitis

Abstract: Topical use of B. lactis HN019 promotes a protective effect against alveolar bone loss and CTALs attributable to EP in rats, modifying immunoinflammatory and microbiologic parameters.

Cited by 85 publications

References 47 publications

Effects of Bifidobacterium probiotic on the treatment of chronic periodontitis: A randomized clinical trial

Effects of Bifidobacterium probiotic on the treatment of chronic periodontitis: A randomized clinical trial

Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats

Chronological analysis of periodontal bone loss in experimental periodontitis in mice

Contact Info

Product

Resources

About