People living in nature need to respect and protect nature in order to build a harmonious and green home. With the growth and progress of society, natural environment protection and ecological construction have become the issues of public concern, and the development of artificial intelligence (AI) and big data (BD) analysis technology has brought more possibilities to environmental protection. Based on this, this paper applied AI and BD analysis technology to the protection of natural environment. This paper first introduced the significance and current situation of natural resources protection and ecological environment construction, and then analyzed the application of AI and BD in environmental protection. Then, the countermeasures of natural environment protection and ecological environment construction were stated. Finally, the effect of natural protection environment and ecological construction was analyzed. It was concluded that after the adoption of AI and BD analysis technology, the pollution index of each nature reserve decreased, all lower than 6 points. The protection effect of each nature reserve has improved. The biomass of each nature reserve has been significantly improved after the adoption of AI and BD analysis technology compared with that before the adoption of AI and BD analysis technology. It can be learned that AI and BD analysis technology can be applied to the protection of natural environment and the construction of ecological environment in the future.