Beneficial hemodynamic effects of autologous bone marrow cell transplantation in rats with heart failure

Tran, Nguyen; Bertrand, Sylvie; Li, Y; Carteaux, Jean‐Pierre; Stoltz, Jean‐François; Villemot, Jean‐Pierre

doi:10.1016/s0041-1345(02)03589-3

Cited by 8 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding a possible treatment of acute myocardial infarction with expanded EPC‐derived cells, the time frame for differentiation from CD34+ cells needs to be considered. Previous experimental studies have shown that myocardial bone marrow transplantation even 4 weeks after the acute event improved perfusion and left ventricular function (33, 34). If this holds true in humans, expansion of sufficient cell numbers seems feasible.…”

Section: Discussionmentioning

confidence: 99%

Endothelial‐like cells expanded from CD34 ⁺ blood cells improve left ventricular function after experimental myocardial infarction

Ott¹,

Keller

Knoedler³

et al. 2005

FASEB j.

102

104

View full text Add to dashboard Cite

Mobilization and recruitment of endothelial progenitor cells (EPC) contributes to vasculogenesis in vivo. So far, applications for cell therapy are limited by the number of available cells. Expansion of EPC or their progeny may, therefore, facilitate its therapeutic use in ischemic disease. The aim of this study was to expand CD34+ EPC-derived progeny from different sources, characterize them, and investigate their potential for use in therapeutic vasculogenesis. CD34+ cells from G-CSF-mobilized peripheral blood (PB) and cord blood (CB) were isolated using immunomagnetic beads and cultured in endothelial cell medium. Cells were expanded up to 16 (PB) and up to 46 (CB) population doublings, respectively. Immunophenotypic and mRNA expression analyses showed a high degree of similarity between the cultured cells and human umbilical vein endothelial cells (HUVEC). By day 14 after transplantation, transplanted human CD31-positive EPC-derived cells were detected. These cells expressed the proliferation marker Ki67 and formed vessel-like structures in ischemic myocardium. Most strikingly, transplantation of EPC-derived cells improved left ventricular function after experimental ischemia, as shown by echocardiography. In conclusion, cells cultured from CD34+ EPC can be expanded in vitro to clinically relevant numbers. In vivo, these cells proliferate, form vascular structures, and improve left ventricular function after experimental myocardial infarction. Therefore, in vitro expanded EPC-derived endothelial cells may be beneficial in the treatment of ischemic disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Endothelial‐like cells expanded from CD34 ⁺ blood cells improve left ventricular function after experimental myocardial infarction

Ott¹,

Keller

Knoedler³

et al. 2005

FASEB j.

102

104

View full text Add to dashboard Cite

show abstract

“…Recently, several investigations have shown that cell labeling with 111 In-oxine allows the body distribution of transplanted cells to be Cell therapy with bone marrow mesenchymal stem cells (BMSCs) was recently reported to improve cardiac monitored in vivo up to 7 days after transplantation (3,11). function in ischemic heart disease (13,15,16,23,24), an observation that might at least partly be explained by…”

Section: Introductionmentioning

confidence: 99%

Short-Term Heart Retention and Distribution of Intramyocardial Delivered Mesenchymal Cells within Necrotic or Intact Myocardium

Tran¹,

Li²,

Maskali

et al. 2006

Cell Transplant

View full text Add to dashboard Cite

Cell therapy with bone marrow mesenchymal stem cells (BMSCs) is a new strategy for treating ischemic heart failure, but data concerning the distribution and retention of transplanted cells remain poor. We investigated the short-term myocardial retention of BMSCs when these cells are directly injected within necrotic or intact myocardium. 111 Indium-oxine-labeled autologous BMSCs were injected within either 1-month-old infarction (n = 6) or normal myocardium (n = 6) from rats. Serial in vivo pinhole scintigraphy was scheduled during 1 week in order to track the implanted cells. The myocardial retention of BMSCs was definitely higher in myocardial infarction than in normal myocardial area (estimated percent retention at 2 h: 63 ± 3% vs. 25 ± 4%, p < 0.001) and the estimated cardiac retention values were unchanged in both groups along the 7 days of follow-up. On heart sections at day 7, labeled BMSCs were still around the injection site and appeared confined to the scarred tissue corresponding either to the infarct area or to the myocardium damaged by needle insertion. BMSCs have a higher retention when they are injected in necrotic than in normal myocardial areas and these cells appear to stay around the injection site for at least a 7-day period.

show abstract

Feasibility of in vivo dual-energy myocardial SPECT for monitoring the distribution of transplanted cells in relation to the infarction site

Tran¹,

Poussier

Franken³

et al. 2006

Eur J Nucl Med Mol Imaging

View full text Add to dashboard Cite

show abstract

Beneficial hemodynamic effects of autologous bone marrow cell transplantation in rats with heart failure

Cited by 8 publications

References 7 publications

Endothelial‐like cells expanded from CD34 ⁺ blood cells improve left ventricular function after experimental myocardial infarction

Endothelial‐like cells expanded from CD34 ⁺ blood cells improve left ventricular function after experimental myocardial infarction

Short-Term Heart Retention and Distribution of Intramyocardial Delivered Mesenchymal Cells within Necrotic or Intact Myocardium

Feasibility of in vivo dual-energy myocardial SPECT for monitoring the distribution of transplanted cells in relation to the infarction site

Contact Info

Product

Resources

About

Beneficial hemodynamic effects of autologous bone marrow cell transplantation in rats with heart failure

Cited by 8 publications

References 7 publications

Endothelial‐like cells expanded from CD34 + blood cells improve left ventricular function after experimental myocardial infarction

Endothelial‐like cells expanded from CD34 + blood cells improve left ventricular function after experimental myocardial infarction

Short-Term Heart Retention and Distribution of Intramyocardial Delivered Mesenchymal Cells within Necrotic or Intact Myocardium

Feasibility of in vivo dual-energy myocardial SPECT for monitoring the distribution of transplanted cells in relation to the infarction site

Contact Info

Product

Resources

About

Endothelial‐like cells expanded from CD34 ⁺ blood cells improve left ventricular function after experimental myocardial infarction

Endothelial‐like cells expanded from CD34 ⁺ blood cells improve left ventricular function after experimental myocardial infarction