Beneficial effects of β-conglycinin on renal function and nephrin expression in early streptozotocin-induced diabetic nephropathy rats

Abstract

The objective of the present study was to investigate the effects of b-conglycinin and soya isoflavones on diabetic nephropathy (DN). DN was induced by an intravenous injection of streptozotocin (25 mg/kg) in spontaneously hypertensive rats. DN rats were divided into a non-diabetic group (C, control group) and three DN groups (D, DN with control diet; B, DN þ control diet with one-eighth of casein replaced by b-conglycinin as the protein source; and I, DN þ control diet with 0·01 % soya isoflavones). After a 4-week experimental period, we found that fasting blood sugar and plasma and kidney advanced glycation end product levels and 24 h urinary protein excretion of the B group were significantly lower than those of the D group and insulin sensitivity and nephrin expression of the B group were significantly higher than those of the D group. In addition, systolic blood pressure, angiotensin-converting enzyme activity, angiotensin II level and plasma TAG level of the B group were significantly lower than those of the D group, whereas only the levels of plasma TAG and thiobarbituric acid-reactive substances of the I group were lower than those of the D group. In conclusion, b-conglycinin may be beneficial for retarding DN progression and this effect cannot be completely explained by its isoflavone content.

Targeting and microenvironment-improving of phenylboronic acid-decorated soy protein nanoparticles with different sizes to tumor

“…SP contains all of the essential amino acids and also offers a good balance among nonpolar amino acid, polar amino acid and charged amino acid. SP has been investigated as the food additives, water emulsions and nutraceutical encapsulation in the form of nanoparticles, microspheres and hydrogels 20-23. It was reported that the hydrolysate of SP had the ability to inhibit angiotensin I-converting enzyme (ACE) which could be used to treat hypertension 24 and the ACE inhibition peptides derived from SP could lower blood pressure of spontaneously hypertensive rats 25.…”

Anti‐Inflammatory and Antioxidative Properties of Isoflavones Provide Renal Protective Effects Distinct from Those of Dietary Soy Proteins against Diabetic Nephropathy

“…β‐conglycinin improved hyperglycaemia and kidney nephron expression in rats with STZ‐induced diabetes. [ 19 ] Soy protein hydrolysates demonstrated inhibitory activity against hypertension. [ 20 ] These data suggest that soy proteins including peptides protect renal glomeruli by inhibition of hyperglycaemia or hypertension in diabetic condition.…”

Dietary low‐fat soy milk powder retards diabetic nephropathy progression via inhibition of renal fibrosis and renal inflammation

“…One scientist proposed that DN caused by type 1 and type 2 diabetes should be considered and treated separately because of diverse pathophysiology . Accumulating evidence indicates that a soybean supplement decreases albuminuria and glycemia in a murine model of insulin‐dependent diabetes . Nevertheless, the effect of dietary soy on an insulin‐independent model is still unclear.…”

“…Some studies showed that soy β‐conglycinin increases glucose uptake in muscle by regulating the AMPK signaling pathway and decreases the blood glucose level in a rat model of STZ‐induced diabetes . Furthermore, β‐conglycinin maintains kidney protein expression in a mouse model of STZ‐induced diabetes . Genistein, a major soy isoflavone, has been reported to have an anti‐inflammatory effect in a model of insulin‐dependent diabetes .…”

“…Treatment with genistein, a major isoflavone in soybeans, suppresses hyperglycemia, oxidative stress, and renal inflammation in an animal model of type 1 diabetes mellitus . Short‐term dietary supplementation with β‐conglycinin also retards DN progression via preservation of kidney protein expression a murine model of streptozotocin (STZ) induced diabetes . These lines of evidence reveal the mechanisms by which pure components of soybeans protect kidney function in insulin‐dependent diabetes mellitus.…”