Beneficial Effects of Pomegranate Peel Extract and Probiotics on Pre-adipocyte Differentiation

Abstract

The beneficial effects of pomegranate are due to the ellagitannins and anthocyanins content, which are protective toward a wide variety of diseases including inflammatory diseases. Many investigators have reported that pomegranate waste (peel and seeds) extracts, made from waste product of industrial processing, show free radical scavenger and a potent antioxidant capacity. Pomegranate extracts (PEs) were also reported to possess noteworty antibacterial, antiviral, hypolipidemic, and anti-inflammatory bioactivities thanks to the polyphenolic compounds content, which includes punicalagins, gallic acid, and ellagic acid derivatives. The focus of the present manuscript was to study the prebiotic potentiality of a PE, soluble in water, and characterized through HPLC–PDA–ESI/MS n for its phenolic content. Moreover, since it has been reported that pomegranate extracts decreased the level of lipids in the blood and that a number of probiotic strains have been shown to affect adipogenesis in cell culture, this study was also performed to test the in vitro effects of PE and probiotic L. rhamnosus GG ATCC 53103 strain (LGG) on 3T3-L1 cell line. PE and probiotics substantially reduced the triglyceride content and intracellular lipid increase, compared to the control group. However, the combination treatment of PE and LGG filtered spent broth (SB) was the most effective in reducing triglyceride content and intracellular lipid accumulation. The mRNA expression levels of the main transcriptional factors implicated in adipocyte differentiation were substantially lower in 3T3-L1 cells treated with PE and LGG filtered SB. These results evidenced that a synergistic effect of probiotics and polyphenols contained in PE may affect in vitro adipogenesis and may contribute in development of new nutraceutical/probiotic-based remedies to prevent and to treat obesity.

Pomegranate Mesocarp against Colitis-Induced Visceral Pain in Rats: Effects of a Decoction and Its Fractions

“…Polysaccharides extracted from pomegranate peels are known to possess excellent antioxidant and immunomodulatory properties [59]. Joseph and colleagues [60] demonstrated that a galactomannan extracted from the peel of Punica granatum L. fruit presents radical scavenging activity [61] and prebiotic effects, stimulating the growth of beneficial bacteria in the colon and maintaining a good state of health of the intestine [62]. In a dextran-sulfate-induced colitis animal model, Yue and colleagues [63] showed that the polysaccharides ameliorated the inflammatory response through lowering TNF-α, IL-1β, IL-6, and MPO activity and increased AMPK activity.…”

Therapeutic Efficacy of Antioxidants in Ameliorating Obesity Phenotype and Associated Comorbidities

“…Previous studies have reported that the pomegranate extract could decrease the level of lipids in the blood with significant anti-inflammatory activities. Recent in vitro study has also demonstrated that the polyphenols contained in pomegranate extracts in combination with probiotics induces synergistic effects that substantially reduces the triglyceride levels and intracellular lipid increase ( Sorrenti et al., 2019 ). The study showed that in vitro treatment with pomegranate extract and probiotics improved the expression of fatty acid synthase, adiponectin, adipogenic markers, PPARγ and SREBP, as well as inflammatory cytokine, IL-6.…”

Synergistic Inhibition of Mycotoxigenic Fungi and Mycotoxin Production by Combination of Pomegranate Peel Extract and Azole Fungicide

“…While a number of recent studies explored the interactions between natural products and antifungal drugs (Shin, 2003; Shin and Kang, 2003; Shin and Lim, 2004; Karioti et al, 2011), the use of such combined antifungal treatments in agricultural setting remains limited, particularly when compared to clinical applications. Pomegranate by-products, such as peel and seeds, are considered a rich source of bioactive compounds such as flavonoids, phenolic acids, and tannins which have free radical scavenging activity and antioxidant capacity (Panichayupakaranant et al, 2010; Sorrenti et al, 2019). Several studies have been reported on the effectiveness of pomegranate peel extracts (PPEs) against human and plant fungal pathogens (Dikmen et al, 2011; Glazer et al, 2012; Foss et al, 2014; Pangallo et al, 2017; Rosas-Burgos et al, 2017).…”