Beneficial effects of melittin on ovalbumin-induced atopic dermatitis in mouse

Abstract

Atopic dermatitis (AD) is an inflammatory skin disease characterized by intense pruritus and relapsable eczematous lesions. The hallmarks of AD are defects in the epidermal barrier and immunoglobulin E (IgE)-mediated sensitization to several environmental allergens, as well as an immune disorder mediated by an imbalance toward T-helper-2 response. Melittin, a major component of bee venom, has been studied in various inflammatory diseases. However, the beneficial effects of melittin on mouse with AD-like symptoms have not been explored. Therefore, we investigated the anti-allergic effects of melittin. AD was induced by ovalbumin (OVA) patch. After agent treatment, skin tissues and sera were extracted from the sacrificed mice were used to demonstrate the effects of melittin through various molecular biological methods. The results showed that OVA-induced skin thickening and inflammatory infiltration were decreased in the melittin-treated group. Melittin prevented OVA-induced filaggrin deficiency and imbalanced inflammatory mediators. Furthermore, melittin inhibited IL-4/IL-13-induced filaggrin downregulation through the blockade of STAT3 activation in human keratinocytes. In summary, this study has shown that melittin ameliorated OVA-induced AD-like symptoms from various perspectives. The findings of this study may be the first evidence of the anti-inflammatory effects of melittin on OVA-induced AD.

Therapeutic effects of bee venom on experimental atopic dermatitis

“…BV has an anti-inflammatory effect on AD in this experiment. In previous study, melittin which is a major component of BV also showed an anti-inflammatory function for AD ( 28 ). It seems to be due to melittin, the main component of BV.…”

“…Induction of AD was performed according to the method given by Kim et al ( 28 ) Briefly, All the mice, with the exception of those in the NC group, were intraperitoneally inoculated mixed with 10 µg of chicken OVA (grade V) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) mixed with 4 mg of aluminum hydroxide (ImjectAlum ® ; Thermo Fisher Scientific, Inc., Waltham, MA, USA) in a volume of 200 µl three time at intervals of one-week intervals (i.e., on days 0, 7, and 14). They were then anesthetized by isoflurane inhalation (Ifran ® ; HANA Pharm, Seoul, Korea).…”

“…The anticancer properties of BV have also been demonstrated in lung and breast cancer, hepatocellular carcinoma, and prostate cancer cells ( 25 – 27 ). In previous study, we demonstrated that melittin of main BV components has beneficial effects on atopic-dermatitis ( 28 ). However, the anti-inflammatory effects of BV on ovalbumin (OVA)-induced inflammatory skin disease in an animal model have not been reported to date.…”

Anti‐fibrotic effects of synthetic TGF‐β1 and Smad oligodeoxynucleotide on kidney fibrosis in vivo and in vitro through inhibition of both epithelial dedifferentiation and endothelial‐mesenchymal transitions

“…Western blotting was performed as described previously. 31 Primary antibodies used in the study were as follows: anti-E-cadherin, vimentin (BD Biosciences), anti-VE-cadherin, TGF-β1, α-SMA, TNF-α, IL-6, S100A4, Fibronectin, collagen Ⅰ (Abcam), antip-Smad2/3, t-Smad2/3, Slug, Snail, t-ERK1/2, p-ERK1/2, YAP/TAZ, p-Akt, p-JNK (cell signaling), anti-Smad4, IL-1β, and TIMP-1 (Santa Cruz Biotechnology). β-actin (Sigma-Aldrich) was used to control the protein loading.…”

Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia

“…Some research reported that the ovalbumin has molecular weight 43 kDa (Fish et al 1969), 45 kDa (Chun et al 2011) or ranging from 43 to 45 kDa (Urisu 2001). This protein has an immunogenic property and used by scientist to induced allergic asthma (Alenius et al 2017) and atopic dermatitis (AD) on an animal model (Kim et al 2017). Ovalbumin protein is containing 385 amino acid residues, which is on 323-339 amino acid residue are have IgEbinding activity and promote histamine release (Johnsen and Elsayed 1990).…”