Beneficial effects of l-carnitine in myoblastic C2C12 cells

Georges, Béatrice; Galland, Stéphane; Rigault, Caroline; Borgne, Françoise Le; Demarquoy, Jean

doi:10.1016/s0006-2952(03)00110-2

Cited by 31 publications

(19 citation statements)

References 16 publications

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“…These findings are consistent with literature reports on diet-induced cardiomyopathy with or without neurological signs such as epilepsy (Barbeau, 1975) in cats (Michel, 2005) and humans (Fosslien, 2003;Georges et al, 2003;Karmaniolas et al, 2002;Vescovo et al, 2005). These cases too are deficient in taurine and/or carnitine due to genetic mutations that affect taurine and carnitine transport or metabolism.…”

Section: Combining Methodological Approachessupporting

confidence: 91%

Methods and biomarkers for the diagnosis and prognosis of cancer and other diseases: Towards personalized medicine

Anderson

Hansen

Mooren

et al. 2006

Drug Resistance Updates

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The rapid development of new diagnostic procedures, the mapping of the human genome, progress in mapping genetic polymorphisms, and recent advances in nucleic acid-and protein chip technologies are driving the development of personalized therapies. This breakthrough in medicine is expected to be achieved largely due to the implementation of "lab-on-the-chip" technology capable of performing hundreds, even thousands of biochemical, cellular and genetic tests on a single sample of blood or other body fluid. Focusing on a few disease-specific examples, this review discusses selected technologies and their combinations likely to be incorporated in the "lab-on-the-chip" and to provide rapid and versatile information about specific diseases entities. Focusing on breast cancer and after an overview of single-nucleotide polymorphism (SNP)-screening methodologies, we discuss the diagnostic and prognostic importance of SNPs. Next, using Duchenne muscular dystrophy (DMD) as an example, we provide a brief overview of powerful and innovative integration of traditional immuno-histochemistry techniques with advanced biophysical methods such as NMR-spectroscopy or Fourier-transformed infrared (FT-IR) spectroscopy. A brief overview of the challenges and opportunities provided by protein and aptamer microarrays follows. We conclude by highlighting novel and promising biochemical markers for the development of personalized treatment of cancer and other diseases: serum cytochrome c, cytokeratin-18 and -19 and their proteolytic fragments for the detection and quantitation of malignant tumor mass, tumor cell turn-over, inflammatory processes during hepatitis and Epstein-Barr virus (EBV)-induced hemophagocytic lymphohistiocytosis and apoptotic/necrotic cancer cell death.

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Section: Combining Methodological Approachessupporting

confidence: 91%

Methods and biomarkers for the diagnosis and prognosis of cancer and other diseases: Towards personalized medicine

Anderson

Hansen

Mooren

et al. 2006

Drug Resistance Updates

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“…In a previous study [19] , we showed that the muscle cell proliferation was limited by L -carnitine bioavailability.…”

mentioning

confidence: 85%

“…The L -carnitine uptake by C2C12 cells was determined as previously described [19] . Briefly, C2C12 cells were plated onto 12-well plates at various densities and used 24 h later.…”

Section: Determination Of L -Carnitine Transporter Activity In C2c12 mentioning

confidence: 99%

Extracellular ATP Increases <i>L</i>-Carnitine Transport and Content in C2C12 Cells

Rigault

Bernard²,

Georges³

et al. 2008

Pharmacology

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Extracellular ATP regulates cell proliferation, muscle contraction and myoblast differentiation. ATP present in the muscle interstitium can be released from contracting skeletal muscle cells. L-Carnitine is a key element in muscle cell metabolism, as it serves as a carrier for fatty acid through mitochondrial membranes, controlling oxidation and energy production. Treatment of C2C12 cells with 1 mmol/l of ATP induced a marked increase in L-carnitine uptake that was associated with an increase in L-carnitine content in these cells. These effects were found to be dependent on the density of the cultured cells and on the dose of ATP. The use of specific inhibitors of P2X and P2Y receptors abolished the effect of ATP on L-carnitine metabolism. As ATP can be released from stressed or exercising cells, it can be hypothesized that ATP acts as a messenger in the muscle. ATP will be released to recruit the next cells and increase their metabolism.

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“…C2C12 cells are myoblastic cells that exhibit most of the characteristics of normal myoblastic cells (13). C2C12 cells are commonly used as a model for studying muscle cell growth and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Nitric Oxide Induces Apoptosis in Mouse C2C12 Myoblast Cells

et al. 2005

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Abstract. To investigate whether nitric oxide (NO) induces apoptosis in myoblast cells, the effect of the sodium nitroprusside (SNP), NO donor, on the apoptosis of mouse C2C12 myoblast cells was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and caspase-3 enzyme assay. Mouse C2C12 myoblast cells treated with SNP exhibited several apoptotic features. SNP increased p53 expression and bax expression. SNP also enhanced caspase-3 enzyme activity. The data show that NO may induce apoptotic cell death in myoblast cells through the activation of p53-, bax-, and caspase-dependent intracellular death-related pathways.

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Beneficial effects of l-carnitine in myoblastic C2C12 cells

Cited by 31 publications

References 16 publications

Methods and biomarkers for the diagnosis and prognosis of cancer and other diseases: Towards personalized medicine

Methods and biomarkers for the diagnosis and prognosis of cancer and other diseases: Towards personalized medicine

Extracellular ATP Increases <i>L</i>-Carnitine Transport and Content in C2C12 Cells

Nitric Oxide Induces Apoptosis in Mouse C2C12 Myoblast Cells

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