Beneficial Effects ofEmblica officinalisinl-Arginine-Induced Acute Pancreatitis in Rats

Abstract

Acute necrotizing pancreatitis is characterized by focal macroscopic or diffuse necrosis, hemorrhage, and vascular thrombosis of the pancreas. Current treatment options are limited to supportive and symptomatic interventions. A large amount of experimental work is ongoing to identify novel therapeutic agents for acute pancreatitis. The present study was carried out to explore the beneficial effects of Emblica officinalis, a medicinal plant of India, on acute pancreatitis. Ascorbic acid is one of the major chemical components of E. officinalis, so a vitamin C group was included for comparison. Acute pancreatitis was induced by L-arginine. Rats were divided into the following groups: control (saline), arginine + saline, arginine + E. officinalis, and arginine + vitamin C. Animals in each group were sacrificed at 24 hours and 3, 14, and 28 days after pancreatitis induction for determination of biochemical parameters and histological examination. For rate of DNA synthesis and immunohistochemical studies, animals were sacrificed on Day 3 and Day 7. Drug administration was started 2 hours after the last arginine injection and continued until the day of sacrifice. E. officinalis treatment was found to be beneficial for treating acute pancreatitis. Serum levels of lipase and interleukin-10 were significantly lower than in the arginine group. Nucleic acid content, rate of DNA synthesis, pancreatic proteins, and pancreatic amylase content were significantly improved. Histopathological examination showed significantly lower total scores in the Emblica group. Vitamin C was found to be less efficacious than E. officinalis for all outcome parameters. Thus E. officinalis treatment was found to be beneficial in acute necrotizing pancreatitis.

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

“…Serum amylase and lipase are the biochemical tests used to diagnose acute pancreatitis (Sidhu et al 2011). Administration of L-arginine produced significantly higher levels of serum amylase at 24 h and day 3.…”

Enhancing the functional properties and nutritional quality of ice cream with processed amla (Indian gooseberry)

“…Amla contains gallic acid which is a potent polyphenol and has been found to improve immunity (Singh et al 2011). It is also a rich source of pectin, an important form of dietary fiber that regulates the bowel action, is anti-diabetic, and treats scurvy and pulmonary tuberculosis (Ganju et al 2003;Yokozawa et al 2007;Sidhu et al 2011). Because of its excellent therapeutic values, it is extensively used in Ayurvedic medicines like Chyavanprash, Triphalahurna, Brahmi rasayan and Madhumehga (Anon 2003).…”

Granulocyte colony-stimulating factor enhances the therapeutic efficacy of bone marrow mesenchymal stem cell transplantation in rats with experimental acute pancreatitis

“…FACS assays confirmed that 97.58% of the isolated cells expressed CD29 (Figure 3A), 0.69% expressed CD34 (a myeloid progenitor cell antigen also presented in endothelial cells and some fibroblasts, Figure 3B); 66.88% expressed CD44 (a stem cell marker, Figure 3C), 2.24% expressed CD45 (a hematopoietic and leukocyte marker, Figure 3D) [21]; and 63.80% of the cells expressed CD90 (Figure 3E). Thus, the cells showed the antigenic profile of BMMSCs [22].…”