Beneficial effects of green coffee and green tea extract combination on metabolic syndrome improvement by affecting AMPK And PPAR-α gene expression

Lukitasari, Mifetika; Nugroho, Dwi Adi; Rohman, Mohammad Saifur; Widodo, Nashi; Farmawati, Arta; Hastuti, Pramudji

doi:10.4103/japtr.japtr_116_19

Cited by 14 publications

(6 citation statements)

References 17 publications

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“…Meanwhile, the combination of green tea and green coffee extract showed substantially higher AMPK-α2 expression compared to that of single extract either green tea or green coffee extract administration (p < 0.05). 32 Therefore, this study revealed that green tea or green coffee extract administration could ameliorate the FFAs levels by improving the adiponectin/adipo-R1/AMPK/FAS pathway in liver tissue rats. However, the combined extract was more effective.…”

Section: Resultsmentioning

confidence: 74%

“…Significant differences in SBP, FBG, TG, and HDL levels were observed between the normal control group (NC) and the metabolic syndrome group (MS) (p < 0.05). 32 The pre-and post-test in all extract-intervention groups showed improvement in SBP, FBG, TG, and HDL levels. Meanwhile, the GC group had no statistically significant difference between pre-and post-test, except the HDL level (data were available in https://doi.org/10.6084/m9.figshare.13249163.v3).…”

Section: Resultsmentioning

confidence: 85%

“…This finding was linear with our previous study that AMPK mRNA expressions were reduced in MS rats. 32 AMPK has an inhibitory effect on FAS 51 ; thus, the reduction of AMPK showed a decrease in inhibitory regulation effect. Nevertheless, green tea or green coffee intervention in the present study showed a decrease in FAS expressions, and these were similar to other previous studies 43,44,49,52,53 ; however, our findings showed no significant difference between green tea and green coffee extract rat groups.…”

Section: Discussionmentioning

confidence: 97%

“…Furthermore, our previous study showed that hepatic AMPK expression was improved in all intervention groups, and the highest level showed in the combination extract group. 32 Lower adiponectin levels and higher FFA levels are related to insulin resistance in hepatic, skeletal, and cardiac tissue. 33,38 This study showed that higher HOMA-IR levels reflected insulin resistance conditions.…”

Section: Discussionmentioning

confidence: 99%

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Green Tea and Decaffeinated Light Roasted Green Coffee Extract Combination Improved Cardiac Insulin Resistance through Free Fatty Acids and Adiponectin/FAS Pathway Amelioration in Metabolic Syndrome Rat Model

et al. 2021

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Background: Insulin resistance has been independently associated with cardiac diseases. A free fatty acid is recently known to induce cardiac insulin resistance due to low-grade inflammation. Therefore, the improvement of free fatty acid levels can also improve cardiac insulin resistance. This study investigated the combination of green tea and decaffeinated-light roasted green coffee extract in improvement of free fatty acid-induced cardiac insulin resistance by improving the adiponectin/FAS pathway. Methods: This study used 25 males Sprague-Dawley rats induced by a high-fat high sucrose diet and injection of low dose streptozotocin to make a metabolic syndrome (MS) rat model and standard chow as healthy control rats. The MS rats were treated with green tea (200 mg/ b. w.), decaffeinated-light roasted green coffee (300 mg/ b. w.), and the combination of both extracts in 9 weeks. Experimental groups in this study were divided into 5 groups: 1) MS (HFHS diet + STZ) group, 2) NC (normal chow) group, 3) GT (green tea extract) group, 4) GC (decaffeinated-light roasted green coffee extract), 5) CM (combination of both extracts) group. Adiponectin and HOMA-IR level was analysed using ELISA, and the gene expression of Adipo-R1, FAS, PI3K, PDK1, Akt, GLUT4 was measured by RT-PCR. Results: The combination of green tea and decaffeinated-light roasted green coffee showed synergistic effects in improving FFA levels. The adiponectin/FAS pathway was attenuated in the CM group. Moreover, the combination also showed improvement in cardiac insulin resistance markers such as IRS1/2, PI3K, PDK1, Akt, and GLUT4. Conclusions: The combination of green tea and decaffeinated-light roasted green coffee extract improved cardiac insulin resistance better than green tea and green coffee extract administration alone by reducing free fatty acids levels through adiponectin/FAS pathway modulation.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Green Tea and Decaffeinated Light Roasted Green Coffee Extract Combination Improved Cardiac Insulin Resistance through Free Fatty Acids and Adiponectin/FAS Pathway Amelioration in Metabolic Syndrome Rat Model

et al. 2021

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“…22 Moreover, this combination showed a synergistic activity based on a previous study. 23 For the present study, we tried to investigate the anti-inflammatory and modulation of lipid influx/efflux effects of decaffeinated coffee and green tea extract (DCGTE) in suppressing foam cell formation.…”

Section: Introductionmentioning

confidence: 99%

Decaffeinated coffee and green tea extract inhibit foam cell atherosclerosis by lowering inflammation and improving cholesterol influx/efflux balance through upregulation of PPARγ and miR-155

et al. 2021

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Background: Foam cells are markers of atherosclerosis and characterise advanced atherosclerotic plaque, stimulated by inflammation caused by high lipid levels in macrophages. The combination of decaffeinated coffee and green tea extract (DCGTE) has been suggested to have a role in foam cell inhibition. Objective: we investigated the inhibiting role of DCGTE against foam cell formation, through modulation of the inflammation process and cholesterol metabolism in macrophage colony stimulating factor- (M-CSF) and oxidized low-density lipoprotein (oxLDL)-exposed macrophages. Methods: Coffee and green tea were extracted by filtration and infusion respectively, and underwent decaffeination using active carbon and blanching methods, respectively. Cells were administered 160/160 and 320/320μg/ml of DCGTE. Foam cell formation was observed using a light microscope after staining with Oil Red O (ORO), and the accumulation of lipids in macrophages with ELISA. Observations of lipid influx and efflux were determined through semiquantitative cluster differentiation 36 (CD36) and ATP binding cassette transporter A1 (ABCA1) expression through immunofluorescence. The inflammation process was quantified using inflammatory/anti-inflammatory markers, e.g., tumor necrosis factor α (TNFα) and interleukin 10 (IL10) with ELISA. Peroxisome proliferator activated response γ (PPARγ) expression and activity were assessed with PCR and ELISA, respectively. The expression of microRNA 155 (miR-155) was examined using qPCR. Results: DCGTE at the above concentrations tended to reduce foam cell numbers, significantly inhibited lipid accumulation (p=0.000), reduced CD36 expression (p=0.000) and TNFα secretion (p=0.000) in Raw264.7 exposed to M-CSF 50ng/ml and oxLDL 50μg/ml. PPARγ expression (p=0.00) and activity (p=0.001), miR-155 relative expression (p=0.000), and IL10 production (p=0.000) also increased. Conclusion: DCGTE lowered foam cell numbers, possibly through attenuation of the inflammatory process and improvement of lipid/efflux mechanisms in M-CSF and oxLDL-stimulated Raw264.7 cells, via upregulation of PPARγ and miR-155. Our results suggest DCGTE may help prevent atherosclerosis-based diseases.

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Evaluation of antidiabetic activity of dietary phenolic compound chlorogenic acid in streptozotocin induced diabetic rats: Molecular docking, molecular dynamics, in silico toxicity, in vitro and in vivo studies

Singh

Rana

Singh

et al. 2021

Computers in Biology and Medicine

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Beneficial effects of green coffee and green tea extract combination on metabolic syndrome improvement by affecting AMPK And PPAR-α gene expression

Cited by 14 publications

References 17 publications

Green Tea and Decaffeinated Light Roasted Green Coffee Extract Combination Improved Cardiac Insulin Resistance through Free Fatty Acids and Adiponectin/FAS Pathway Amelioration in Metabolic Syndrome Rat Model

Green Tea and Decaffeinated Light Roasted Green Coffee Extract Combination Improved Cardiac Insulin Resistance through Free Fatty Acids and Adiponectin/FAS Pathway Amelioration in Metabolic Syndrome Rat Model

Decaffeinated coffee and green tea extract inhibit foam cell atherosclerosis by lowering inflammation and improving cholesterol influx/efflux balance through upregulation of PPARγ and miR-155

Evaluation of antidiabetic activity of dietary phenolic compound chlorogenic acid in streptozotocin induced diabetic rats: Molecular docking, molecular dynamics, in silico toxicity, in vitro and in vivo studies

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