Beneficial effects of Galectin-9 on allergen-specific sublingual immunotherapy in a Dermatophagoides farinae -induced mouse model of chronic asthma

Ikeda, Masaki; Katoh, Shigeki; Shimizu, Hiroki; Hasegawa, Akira; Ohashi-Doi, Katsuyo; Oka, M.

doi:10.1016/j.alit.2016.10.007

Cited by 17 publications

(15 citation statements)

References 26 publications

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“…Two of these proteins are transketolase (UniProtKB ID P40142) that affects the NAPDH production in order to counteract oxidative stress [ 75 ], and RNA polymerase I-specific transcription initiation factor RRN3 (UniProtKB ID B2RS91) which acts as a connector between RNA polymerase I and transcription factors [ 76 ]. Nevertheless, such a unique succinylated lysine was only detected by iSuc-PseOpt [ 13 ], SuccinSite [ 16 ] and pSuc-Lys [ 14 ] predictors for proteins such as galectin (UniProtKB ID B1AQR8), which acts as an immunomodulatory and enhances transforming growth factor-b signaling [ 77 ]. For other proteins, their only succinylated lysine was discovered by all the predictors.…”

Section: Resultsmentioning

confidence: 99%

Success: evolutionary and structural properties of amino acids prove effective for succinylation site prediction

et al. 2018

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BackgroundPost-translational modification is considered an important biological mechanism with critical impact on the diversification of the proteome. Although a long list of such modifications has been studied, succinylation of lysine residues has recently attracted the interest of the scientific community. The experimental detection of succinylation sites is an expensive process, which consumes a lot of time and resources. Therefore, computational predictors of this covalent modification have emerged as a last resort to tackling lysine succinylation.ResultsIn this paper, we propose a novel computational predictor called ‘Success’, which efficiently uses the structural and evolutionary information of amino acids for predicting succinylation sites. To do this, each lysine was described as a vector that combined the above information of surrounding amino acids. We then designed a support vector machine with a radial basis function kernel for discriminating between succinylated and non-succinylated residues. We finally compared the Success predictor with three state-of-the-art predictors in the literature. As a result, our proposed predictor showed a significant improvement over the compared predictors in statistical metrics, such as sensitivity (0.866), accuracy (0.838) and Matthews correlation coefficient (0.677) on a benchmark dataset.ConclusionsThe proposed predictor effectively uses the structural and evolutionary information of the amino acids surrounding a lysine. The bigram feature extraction approach, while retaining the same number of features, facilitates a better description of lysines. A support vector machine with a radial basis function kernel was used to discriminate between modified and unmodified lysines. The aforementioned aspects make the Success predictor outperform three state-of-the-art predictors in succinylation detection.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4336-8) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Success: evolutionary and structural properties of amino acids prove effective for succinylation site prediction

et al. 2018

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“…In fact, intravenous, intranasal, or sublingual administration of Gal-9 has been shown to be an important therapeutic tool in experimental models of asthma [25,31,32]. In these studies, treatment with Gal-9 reduced hyperactivity of respiratory epithelial cells, hypersecretion of mucus, leukocyte infiltration into the lungs, and BALF.…”

Section: Discussionmentioning

confidence: 99%

The Role of Galectin-9 as Mediator of Atopic Dermatitis: Effect on Keratinocytes

Corrêa

Areias

Correia-Silva

et al. 2021

Cells

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Galectin-9 (Gal-9) is a beta-galactoside-binding protein with a variety of biological functions related to immune response. However, in allergic diseases, its mechanism of action is not fully understood. This study evaluates the expression pattern of Gal-9 in patients with atopic dermatitis (AD), in ovalbumin (OVA)-induced experimental atopic dermatitis (AD) in mice, as well as its effect on human keratinocytes. The skin of OVA-immunized BALB/c mice was challenged with drops containing OVA on days 11, 14–18, and 21–24. HaCaT cells were cultured in the following experimental conditions: control (growth medium only) or stimulated with TNF-α/IFN-γ, or IL-4, or IL-17 with or without Gal-9 treatment. AD was characterized by increased levels of Gal-9 in mouse and human skin, especially in the epidermis, and with a marked influx of Gal-9 positive eosinophils and mast cells compared to the control group. Gal-9 showed an immunomodulatory effect on keratinocytes by decreasing the release of IL-6 by IL-4-stimulated keratinocytes or increasing the IL-6 and RANTES levels by IL-17- or TNF-α/IFN-γ-stimulated cells, respectively. Under IL-17, Gal-9 treatment also altered the proliferation rate of cells. Overall, increased levels of Gal-9 in AD skin contribute to the control of inflammatory response and the proliferative process of keratinocytes, suggesting this lectin as a relevant therapeutic target.

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“…Especially, galectin-9 binds directly to CD44 and promotes Foxp3 expression of Tregs, which have been reported to suppress excessive Th2 responses (32). Indeed, recombinant galectin-9 treatment resulted in improved clinical and immunological symptoms of allergic diseases in mouse models (33). Recently, Bifidobacterium breve M-16V, used as a dietary supplement, was shown to induce elevation of serum galectin-9 in mice and humans (34).…”

Section: Introductionmentioning

confidence: 99%

Galectin-9 Induced by Dietary Probiotic Mixture Regulates Immune Balance to Reduce Atopic Dermatitis Symptoms in Mice

Kim

Kye

et al. 2020

Front. Immunol.

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Beneficial effects of Galectin-9 on allergen-specific sublingual immunotherapy in a Dermatophagoides farinae -induced mouse model of chronic asthma

Abstract: Gal-9 exhibited beneficial effects of sublingual Df allergen-specific immunotherapy in a Df-induced mouse model of chronic asthma, possibly by Gal-9-induced TGF-β1 production in the lung.

Cited by 17 publications

References 26 publications

Success: evolutionary and structural properties of amino acids prove effective for succinylation site prediction

Success: evolutionary and structural properties of amino acids prove effective for succinylation site prediction

The Role of Galectin-9 as Mediator of Atopic Dermatitis: Effect on Keratinocytes

Galectin-9 Induced by Dietary Probiotic Mixture Regulates Immune Balance to Reduce Atopic Dermatitis Symptoms in Mice

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