Beneficial effect of ovocystatin on the cognitive decline in APP/PS1 transgenic mice

Stańczykiewicz, Bartłomiej; Jakubik-Witkowska, Marta; Rutkowská, M; Polanowski, Antoni; Gburek, Jakub; Gołąb, Krzysztof; Juszczyńska, Katarzyna; Trziszka, Tadeusz; Rymaszewska, Joanna

doi:10.1016/j.advms.2018.08.002

Cited by 4 publications

(4 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown up until now, that ovocystatin has a beneficial impact on cognitive function in young rats, and might prevent aging-related cognitive impairment in older animals [ 21 ] and reduce memory decline in APP/PS1 mice model [ 23 , 24 ]. However, its potential molecular mechanism of action is not explained and needs to be examined in detail.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, ovocystatin improves cognitive function in young rats [ 21 ] and prevents aging-related cognitive impairment in older animals [ 22 ]. Moreover, recently published studies showed that the administration of ovoC in drinking water might be effective in the prevention of cognitive deterioration in APP/PS1 mice [ 23 ]. Additionally, ovoC could also be applied as a useful factor against Aβ oligomerization and consequent amyloid fibril formation and tau protein deposition [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Ovocystatin on Amyloid β 1-42 Aggregation—In Vitro Studies

Stańczykiewicz

Goszczyński

Migdał

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Amyloid β peptides (Aβ) aggregating in the brain have a potential neurotoxic effect and are believed to be a major cause of Alzheimer’s disease (AD) development. Thus, inhibiting amyloid polypeptide aggregation seems to be a promising approach to the therapy and prevention of this neurodegenerative disease. The research presented here is directed at the determination of the inhibitory activity of ovocystatin, the cysteine protease inhibitor isolated from egg white, on Aβ42 fibril genesis in vitro. Thioflavin-T (ThT) assays, which determine the degree of aggregation of amyloid peptides based on fluorescence measurement, circular dichroism spectroscopy (CD), and transmission electron microscopy (TEM) have been used to assess the inhibition of amyloid fibril formation by ovocystatin. Amyloid beta 42 oligomer toxicity was measured using the MTT test. The results have shown that ovocystatin possesses Aβ42 anti-aggregation activity and inhibits Aβ42 oligomer toxicity in PC12 cells. The results of this work may help in the development of potential substances able to prevent or delay the process of beta-amyloid aggregation—one of the main reasons for Alzheimer’s disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Ovocystatin on Amyloid β 1-42 Aggregation—In Vitro Studies

Stańczykiewicz

Goszczyński

Migdał

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has also been revealed that the survival of the mice during the 6-month study period suggests that ovocystatin might be safe to use. Nevertheless, toxicological studies are warranted to confirm this hypothesis [ 113 ]. In order to ascertain the underlying molecular mechanisms, a histopathological examination was conducted, revealing that both β-amyloid and tau protein deposits were significantly reduced in the hippocampi of the APP/PS1 mice treated intraperitoneally with ovocystatin [ 114 ].…”

Section: Cystatin Cmentioning

confidence: 99%

Cystatins: unravelling the biological implications for neuroprotection

Stańczykiewicz,

Łuc,

Banach

et al. 2023

Arch Med Sci

View full text Add to dashboard Cite

Cystatins, a family of proteins known for their inhibitory role against cysteine proteases, have garnered significant attention in the field of neurodegeneration. Numerous genetic, experimental and clinical studies concerning cystatin C suggest it plays an important role in the course of neurodegenerative diseases. Its beneficial effects are associated with cysteine protease inhibition, impact on β-amyloid aggregation as well as regulation of cell proliferation, autophagy and apoptosis. Cystatin isolated from chicken egg white, called Ovocystatin, has been widely used in medical and pharmaceutical research due to its structural and biological similarities to human cystatin C. This article focuses on the potential use of cystatins, with special emphasis on easily-obtained Ovocystatin, in the treatment of neurodegenerative diseases, such as dementia. The current evidence on cystatin use has been portrayed shedding light on their mechanisms of action and therapeutic implications for neuroprotection and maintenance of cognitive functions.

show abstract

“…Treatment with egg white cystatin has a benefi cial effect on the cognitive functions in APP/PS1 transgenic mice. The strongest effects, measured by swimming ability in the target zone in the Morris water maze, were found in mice drinking water supplied with 40 μg of cystatin [90].…”

Section: Cystatins As Disease Biomarkersmentioning

confidence: 99%

Egg White Cystatin – A Review

et al. 2020

View full text Add to dashboard Cite

Hen eggs are widely used, not only for human consumption, but also as an important material in food production and in pharmaceutical and cosmetics industry. Cystatin is a biologically active component of egg white, mostly used as an inhibitor of papain-like cysteine proteases. It was isolated from chicken egg white and has later been used in the nomenclature of structurally and functionally related proteins. Cystatins from animals, including mouse, rat, dog, cow and chicken egg white have been isolated and recently used in foodstuffs and drug administration. Cystatin has found its place and use in medicine due to its antimicrobial, antiviral and insecticidal effects, for the prevention of cerebral hemorrhage and control of cancer cell metastasis.

show abstract

Beneficial effect of ovocystatin on the cognitive decline in APP/PS1 transgenic mice

Cited by 4 publications

References 54 publications

Effect of Ovocystatin on Amyloid β 1-42 Aggregation—In Vitro Studies

Effect of Ovocystatin on Amyloid β 1-42 Aggregation—In Vitro Studies

Cystatins: unravelling the biological implications for neuroprotection

Egg White Cystatin – A Review

Contact Info

Product

Resources

About