Beneficial Effect of Lantibiotic Nisin in Rabbit Husbandry

Abstract

Nisin is a bacteriocin marketed as Nisaplin. The aim of our work was to test its in vivo effect in a rabbit model; its effect on phagocytic activity (PA) and morphometry has not so far been studied. Post-weaning rabbits (48), 5 weeks old (both sexes, Hycole breed), were divided into the experimental (E) and the control groups (C), 24 animals in each. They were fed a commercial diet with access to water ad libitum. Rabbits in E had nisin additionally administered to their drinking water (500 IU-20 μg per animal/day) for 28 days. The experiment lasted 42 days. On day 28, significant decrease in coagulase-positive (CoPS) staphylococci and coliforms was noted (p < 0.01) in faeces of group E compared with C. Pseudomonads and clostridiae were also significantly reduced (p < 0.001; p < 0.05) and slight decrease was also in CoNS and enterococci. On day 42, coliforms were still significantly reduced (p < 0.001) in faeces; slight decrease in CoPS and pseudomonads was noted. In the caecum, significant decrease in pseudomonads (p < 0.05) was noted on day 28; slight decrease in coliforms. In the appendix slight decrease in coliforms, pseudomonads was obtained on both days. PA was increased significantly in E on days 28, 42 (p < 0.001). Biochemical parameters were not influenced; nor were volatile fatty acids or lactic acid in the chymus. Nisin application did not evoke oxidative stress. In group E, an increase in average body weight gain (about 9.4 %) was noted. The villus height/crypt depth ratio was not influenced; that is, resorption surface and functionality of mucosa were not influenced.

Dietary supplementation of a bacteriocinogenic and probiotic strain of Enterococcus faecium CCM7420 and its effect on the mineral content and quality of Musculus longissimus dorsi in rabbits

“…The acidic condition can enhance the ionisation of minerals, which in turn results in passive diffusion (Cummings et al 2001). The increased iron content could also be explained by the enlargement of the absorption surface due to enterocyte proliferation; improved morphometry parametersvillus height, crypt depth, villus height : crypt depth ratiowere also recorded during CCM7420 administration (Pogány Simonová et al 2015) as well as in our previous experiment with lantibioticnisin application to rabbits (Lauková et al 2014). Copper is an essential trace mineral, which performs important biochemical functions.…”

Can Enterocin M in Combination with Sage Extract Have Beneficial Effect on Microbiota, Blood Biochemistry, Phagocytic Activity and Jejunal Morphometry in Broiler Rabbits?

“…Lower counts of coliforms were noted during sage administration alone and in combination with EntM, similarly to Szabóová et al [19], who reported reduction in E. coli due to sage extract administration in rabbits. Coliform bacteria were also inhibited using EntCCM4231 in combination with sage [17] and during administration of lantibiotics nisin and gallidermin to rabbits [13,42]. Kritas et al [3] also isolated E. coli and Clostridium perfringens at lower frequency from probiotic-treated rabbits.…”

“…They have traditionally been used as food preservatives, either added or produced by starter cultures during fermentation. Moreover, the most often used bateriocins, nisin and pediocin, have been used in other ecosystems: Feed (silage), animals, aquacultures, and also humans [11][12][13]. Enterocins represent an individual group of bacteriocins which are produced by different species of enteroccocci, but mainly by Enterococcus faecium, E. faecalis, E. hirae, E. durans, and E. mundtii [10,14].…”

Nisin as a Novel Feed Additive: The Effects on Gut Microbial Modulation and Activity, Histological Parameters, and Growth Performance of Broiler Chickens

“…In contrast, it is well documented that the use of nisin encapsulated with lipid or bacteriocin without any protection layer may improve the growth of broiler chickens and cause positive GIT microbiota modulations at similar levels [15,16]. In addition, the ability of nisin to change the microbiota was also observed in mice, rats, rabbits, and ruminants [17][18][19][20]. This is in agreement with the results of an in vitro study that showed that partially digested nisin may maintain its activity against pathogenic bacteria; however, the length of the peptide chain has a crucial effect on bacterial growth inhibition [21,22].…”