Beneficial effect of Indigo Naturalis on acute lung injury induced by influenza A virus

Tu, Peng; Tian, Rong; Lu, Yan; Zhang, Yunyi; Zhu, Haiyan; Ling, Liuyi; Li, Hong; Chen, Daofeng

doi:10.1186/s13020-020-00415-w

Cited by 18 publications

(11 citation statements)

References 79 publications

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“…In addition, PFWE decreased the gene expression and protein production of inflammatory cytokine (IL-6) and chemokine (CCL2/MCP-1). Although this is the first study to show the efficacy of PFWE in controlling ARDS, similar results have been reported by applying other TCM, such as Indigo naturalis , Huang Qin, or Sophorae flavescentis Radix [ 21 – 23 ]. Our data further support the use of TCM for the treatment of pulmonary diseases in the acute phase.…”

Section: Discussionsupporting

confidence: 79%

Perilla Fruit Water Extract Attenuates Inflammatory Responses and Alleviates Neutrophil Recruitment via MAPK/JNK-AP-1/c-Fos Signaling Pathway in ARDS Animal Model

Ting

Chen

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Airway respiratory distress syndrome (ARDS) is usually caused by a severe pulmonary infection. However, there is currently no effective treatment for ARDS. Traditional Chinese medicine (TCM) has been shown to effectively treat inflammatory lung diseases, but a clear mechanism of action of TCM is not available. Perilla fruit water extract (PFWE) has been used to treat cough, excessive mucus production, and some pulmonary diseases. Thus, we propose that PFWE may be able to reduce lung inflammation and neutrophil infiltration in a lipopolysaccharide (LPS)-stimulated murine model. C57BL/6 mice were stimulated with LPS (10 μg/mouse) by intratracheal (IT) injection and treated with three doses of PFWE (2, 5, and 8 g/kg) by intraperitoneal (IP) injections. To investigate possible mechanisms, A549 cells were treated with PFWE and stimulated with LPS. Our results showed that PFWE decreased airway resistance, neutrophil infiltration, vessel permeability, and interleukin (IL)-6 and chemokine (C-C motif) ligand 2 (CCL2/MCP-1) expressions in vivo. In addition, the PFWE inhibited the expression of IL-6, CCL2/MCP-1, chemokine (CXC motif) ligand 1 (CXCL1/GROα), and IL-8 in vitro. Moreover, PFWE also inhibited the MAPK/JNK-AP-1/c-Fos signaling pathway in A549 cells. In conclusion, we demonstrated that PFWE attenuated pro-inflammatory cytokine and chemokine levels and downregulated neutrophil recruitment through the MAPK/JNK-AP-1/c-Fos pathway. Thus, PFWE can be a potential drug to assist the treatment of ARDS.

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Section: Discussionsupporting

confidence: 79%

Perilla Fruit Water Extract Attenuates Inflammatory Responses and Alleviates Neutrophil Recruitment via MAPK/JNK-AP-1/c-Fos Signaling Pathway in ARDS Animal Model

Ting

Chen

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…In situ dyeing not only simplifies the dyeing process, but also does not require reducing agents, such as sodium hydrosulfite, which is necessary for traditional textile dyeing [70] . The dyed wool textiles displayed visually more uniform colors than other reports of in situ dyeing (Figure S6) [5,9,29] . Additionally, the dyed color did not fade from the wool textiles after washing with water three times.…”

Section: Resultsmentioning

confidence: 93%

“…Indigo is also the basic backbone of many food colorants, such as indigo carmine, a synthetic indigo derivative that is also used as a colorant in daily consumed foods, including fruit juices, tomato ketchup, bread, and dairy. [2,3] Indigo also has antibacterial, anti-inflammatory, and anticancer effects; [4][5][6][7] for example, it is the main active component of isatidis, which can effectively treat influenza. In recent years, indigoids have been recognized as promising functional and biodegradable nanomaterials for preparing "green electronics".…”

Section: Introductionmentioning

confidence: 99%

Evolving a P450BM3 Peroxygenase for the Production of Indigoid Dyes from Indoles

et al. 2022

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Herein, we describe an environmentally benign enzymatic approach for the preparation of indigoid from indole derivatives. A series of beneficial P450BM3 mutants were obtained using a stepwise approach involving site‐directed, random, and combinatory hot‐site mutations. Using H2O2 as the terminal oxidant and N‐(ω‐imidazolyl)‐hexanoyl‐L‐phenylalanine as a co‐catalyst, the quadruple‐mutant F87G/T268V/F77I/E140D efficiently catalyzed the 3‐hydroxylation of indole and subsequent autooxidation to indigo with higher efficiency than the flavin‐containing monooxygenase, PTDH‐mFMO, which was the best oxidizing enzyme for indigo synthesis reported to date. Following this procedure, 12 indigoid compounds were prepared using indole derivatives with various substituents as starting materials with moderate to high isolated yields (31.3–79.5 %). The catalytic efficiencies (kcat/Km) of the beneficial mutants for typical indole substrates ranged from 182–2849 mM−1 ⋅ min−1, almost 2‐ to 712‐fold higher than those of the reported P450 enzymes. This study provides a new enzymatic approach for the biosynthesis of indigoid dyes from indole derivatives.

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“…Moreover, oral drug administration exacerbates the metabolic burden in the liver, which might result in metabolic disturbances and thereby increasing the susceptibility to carcinogenesis [ 86 ]. Issues regarding hepatotoxicity have been resolved by the promoted liver regeneration by BT [ 87 ], PP [ 88 ], BD [ 89 ], CH [ 90 , 91 ], LC [ 92 , 93 ], SK [ 94 ], and CWT [ 95 ]. ③ IBD patients are susceptible to malnutrition including anemia, amino acid, and vitamin deficiency [ 96 ], which could be rescued by PP [ 97 ] due to its hematopoiesis-promoting effect [ 98 – 100 ] and by the addition of pearl that is produced from shellfish mollusks which contain 20 amino acids and Fe and Mg elements [ 101 ].…”

Section: Discussionmentioning

confidence: 99%

Validation of the Anticolitis Efficacy of the Jian-Wei-Yu-Yang Formula

Yan

Tang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Inflammatory bowel disease (IBD) is a major cause of morbidity and mortality due to its repetitive remission and relapse. The Jian-Wei-Yu-Yang (JW) formula has a historical application in the clinic to combat gastrointestinal disorders. The investigation aimed to explore the molecular and cellular mechanisms of JW. Methods. 2% dextran sodium sulfate (DSS) was diluted in drinking water and given to mice for 5 days to establish murine models of experimental colitis, and different doses of JW solution were administered for 14 days. Network pharmacology analysis and weighted gene co-expression network analysis (WGCNA) were utilized to predict the therapeutic role of JW against experimental colitis and colitis-associated colorectal cancer (CAC). 16S rRNA sequencing and untargeted metabolomics were conducted using murine feces. Western blotting, immunocytochemistry, and wound healing experiments were performed to confirm the molecular mechanisms. Results. (1) Liquid chromatography with mass spectrometry was utilized to confirm the validity of the JW formula. The high dose of JW treatment markedly attenuated DSS-induced experimental colitis progression, and the targets were enriched in inflammation, infection, and tumorigenesis. (2) The JW targets were related to the survival probability in patients with colorectal cancer, underlying a potential therapeutic value in CRC intervention. (3) Moreover, the JW therapy successfully rescued the decreased richness and diversity of microbiota, suppressed the potentially pathogenic phenotype of the gut microorganisms, and increased cytochrome P450 activity in murine colitis models. (4) Our in vitro experiments confirmed that the JW treatment suppressed caspase3-dependent pyroptosis, hypoxia-inducible factor 1α (HIF1α), and interleukin-1b (IL-1b) in the colon; facilitated the alternative activation of macrophages (Mφs); and inhibited tumor necrosis factor-α (TNFα)-induced reactive oxygen species (ROS) level in intestinal organoids (IOs). Conclusion. The JW capsule attenuated the progression of murine colitis by a prompt resolution of inflammation and bloody stool and by re-establishing a microbiome profile that favors re-epithelization and prevents carcinogenesis.

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Beneficial effect of Indigo Naturalis on acute lung injury induced by influenza A virus

Cited by 18 publications

References 79 publications

Perilla Fruit Water Extract Attenuates Inflammatory Responses and Alleviates Neutrophil Recruitment via MAPK/JNK-AP-1/c-Fos Signaling Pathway in ARDS Animal Model

Perilla Fruit Water Extract Attenuates Inflammatory Responses and Alleviates Neutrophil Recruitment via MAPK/JNK-AP-1/c-Fos Signaling Pathway in ARDS Animal Model

Evolving a P450BM3 Peroxygenase for the Production of Indigoid Dyes from Indoles

Validation of the Anticolitis Efficacy of the Jian-Wei-Yu-Yang Formula

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