Beneficial effect of fibronectin administration on chronic nephritis in rats

Quirós, J.F.B. de; González-Cabrero, J; Egido, Jesús; Herrero‐Beaumont, Gabriel; Martínez-Montero, Juan Carlos

doi:10.1002/art.1780330511

Cited by 11 publications

(7 citation statements)

References 26 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Admin istration of Fn alone or in conjunction with immunosuppressors could reduce the deposition of circulating immune complexes or influence cellular immune response and residential kidney cells and, therefore, reduce tissue injury [34]. There is a strong need for more investigation in this field and trials for new perspectives in the therapy of chronic GN.…”

Section: Discussionmentioning

confidence: 99%

Role of Fibronectin in Immune Glomerulonephritis

Altŭnkova¹,

Minkova

Belovezhdov³

1993

Nephron

View full text Add to dashboard Cite

There is already a considerable amount of evidence suggesting that fibronectin (Fn) plays an important role in the pathogenic process in some forms of glomerulonephritis (GN). It has been postulated that Fn may participate in the progression or regression of glomerular diseases. The Fn is presented in the kidney as a normal component of the mesangium, and it is increased in the expanded mesangium in various forms of GN. This paper reports our efforts to investigate the role of Fn in plasma and kidney in patients with GN. Using monoclonal antibodies against human Fn in the ELISA and immunohistoperoxidase techniques to evaluate Fn, we investigated its quantity in connection with clinical state and morphological findings. We studied 93 patients with GN and 26 renal biopsies. The patients with active forms of mesangial proliferative, membranoproliferative and membranous GN showed increased plasma Fn, and the highest levels were in patients with nephrotic syndrome. Increased tissue Fn correlated with mesangial expansion and with IgG and C3 deposits. We speculate on possible mechanisms of the involvement of Fn in human chronic GN.

show abstract

Section: Discussionmentioning

confidence: 99%

Role of Fibronectin in Immune Glomerulonephritis

Altŭnkova¹,

Minkova

Belovezhdov³

1993

Nephron

View full text Add to dashboard Cite

show abstract

“…particularly collagen. from the circulation [29]. A fragment of FN has been proposed to possess immunosuppressive function [30].…”

Section: Lvtnphocvte Interaction With Exogenous Lahelledfihronectinmentioning

confidence: 99%

Fibronectin at the Lymphocyte Surface. Evidence for Activation‐Dependent Binding to VLA4 and VLA5 Integrins

Hauzenberger

Sundqvist

1993

Scand J Immunol

View full text Add to dashboard Cite

The surface of in vitro cultured fixed and viable human T lymphocytes and certain T-cell lines was found to react with different monoclonal anti-fibronectin (FN) antibodies as revealed by ELISA, immunocytochemistry and FACS analysis. SDS-PAGE showed that anti-FN antibodies defined a high molecular weight lymphocyte component which could be iodinated using the lactoperoxidase method and which had gelatin binding capacity. FACS analysis showed that the reactivity of anti-FN antibodies with lymphocytes was most pronounced in activated cells and increased during the culture period. By contrast, FACS analysis revealed equal high expression of the VLA4 and VLA5 integrins on freshly purified as well as on mixed lymphocyte culture (MLC) activated cells. Freshly purified lymphocytes and lymphocytes cultured in vitro overnight did not bind 3H-labelled FN in solution whereas MLC-activated cells were capable of 'spontaneous' binding of such [3H]-FN. However, brief 12-o-tetradecanoylphorbol-13-acetate (TPA) exposure rendered freshly purified lymphocytes capable of binding soluble FN. These interactions of the lymphocytes with 3H-labelled FN in solution could be almost completely blocked by monoclonal anti-VLA4 and VLA5 antibodies. These results indicate that activated T cells express fibronectin at their surface under 'normal' culture conditions. Although both freshly purified and MLC-activated lymphocytes have equal expression of the integrins VLA4 and VLA5, only activated cells are capable of 'spontaneous' binding FN in solution via an integrin-mediated process, probably via an increase in the affinity of these receptors for FN.

show abstract

“…Increasing the serum level of fibronectin can be beneficial sometimes. For example, in rats with chronic nephritis fibronectin administration has resulted in increased immune complex catabolism and a reduction of histological lesions (62).…”

Section: Fibronec'i"mentioning

confidence: 99%