Beneficial Bacteria Affect Danio rerio Development by the Modulation of Maternal Factors Involved in Autophagic, Apoptotic and Dorsalizing Processes

Miccoli, Andrea; Gioacchini, Giorgia; Maradonna, Francesca; Benato, Francesca; Skobo, Tatjana; Carnevali, Oliana

doi:10.1159/000373983

Cited by 22 publications

(21 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 47 in the present study, the lower TAG content found in zebrafish fed a HFD-P and MFD-P suggests the probiotic’s capability to modulate transcriptional process involved in the regulation of TAG metabolism. Contrary to what previously found in zebrafish larvae 10 , 48 , the findings we obtained with adult zebrafish are supported by the loss of weight observed in the adult fish fed a HDF-P and MFD-P compared to the control ones and suggest the capability of the probiotic to successfully prevent weight gain, thus reducing the high-fat-diet induced obesity.…”

Section: Discussioncontrasting

confidence: 94%

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

Falcinelli

Rodiles

Hatef

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

In the present study, we explored whether dietary lipid content influences the gut microbiome in adult zebrafish. Diets containing three different lipid levels (high [HFD], medium [MFD], and low [LFD]) were administered with or without the supplementation of Lactobacillus rhamnosus (P) to zebrafish in order to explore how the dietary lipid content may influence the gut microbiome. Dietary lipid content shifted the gut microbiome structure. The addition of L. rhamnosus in the diets, induced transcriptional reduction of orexigenic genes, upregulation of anorexigenic genes, and transcriptional decrease of genes involved in cholesterol and triglyceride (TAG) metabolism, concomitantly with lower content of cholesterol and TAG. Probiotic feeding also decreased nesfatin-1 peptide in HFD-P and attenuated weight gain in HFD-P and MFD-P fed zebrafish, but not in LFD-P group. Intestinal ultrastructure was not affected by dietary fat level or probiotic inclusion. In conclusion, these findings underline the role of fat content in the diet in altering gut microbiota community by shifting phylotype composition and highlight the potential of probiotics to attenuate high-fat diet-related metabolic disorder.

show abstract

Section: Discussioncontrasting

confidence: 94%

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

Falcinelli

Rodiles

Hatef

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Autophagy is the primary intracellular catabolic process wherein malfunctioning cytoplasmic proteins or disabled organelles are delivered to the lysosomes for degradation and recycling [1,2]. Basal levels of autophagy have been demonstrated to be involved in certain types of physiologic conditions.…”

Section: Introductionmentioning

confidence: 99%

The Role of Probiotics in Lipopolysaccharide-Induced Autophagy in Intestinal Epithelial Cells

Han

Zhang

Shi

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Dysfunction of autophagy has been associated with loss of intestinal homeostasis. Lipopolysaccharide (LPS) from Gram-negative bacteria is known to be a major initiator of intestinal epithelial cell (IEC) autophagy. Although probiotics have been recognized to be involved in many therapeutic properties and participate in host defense responses, the molecular mechanisms by which probiotics exert these positive effects remain unknown. This study assessed the effect of probiotics on LPS-induced physical barrier dysfunction and the underlying mechanism of probiotic action in IECs with a focus on autophagy. Methods: A LPS-induced autophagic model was established in rat IEC18 cells wherein cells were treated with culture medium supernatants of Bifidobacteria following LPS intervention at indicated times. Autophagosomes in IEC18 cells were visualized by confocal microscopy after transfection with a tandem GFP-mCherry-LC3 construct and also by transmission electron microscopy. Autophagy-associated protein levels were analyzed by western blot and transepithelial electrical resistance (TEER) was measured using an epithelial voltohmmeter. Results: Probiotic treatment could effectively inhibit LPS-induced autophagy, as evidenced by the decreased ratio of microtubule-associated light chain 3 (LC3)-II/LC3-I, fewer autophagic vacuoles, and reduced punctate distribution of GFP-mCherry-LC3. In addition, probiotics prevented chloroquine (CQ) inhibition of autophagic flux and autophagolysosomal fusion as indicated by a failure to recruit LAMP1 and cathepsin D to lysosomes. Interestingly, ATG16L1 knockdown did not inhibit the effect of probiotics on LPS-induced autophagy. Furthermore, the diminished barrier function could be prevented by probiotics. Conclusions: We provide evidence that autophagy mediation by probiotics may be involved in enteroprotection against LPS-induced intestinal epithelial toxicity, and could serve as a novel mechanism through which probiotics promote and maintain gut homeostasis.

show abstract

“…Apoptosis is associated with mitophagy 30,31 . Knockdown of Drfundc1 decreased expression of the apoptosis-related genes baxb , bcl2a , bcl2b , and caspase3a 32,33,45,47,48 , contrasting to results of 293 T cells due to ectopic expression of Drfundc1 . This means that Drfundc1 knockdown interferes with apoptosis in zebrafish embryos.…”

Section: Discussionmentioning

confidence: 74%

“…Authors of several studies have claimed that autophagy tends to be accompanied by a certain degree of apoptosis 30,31 . We therefore measured expressions of the following autophagy- and apoptosis-related genes: atg5 , atg7 , atg12 , autophagy and beclin 1 regulator 1a ( ambra1a ), ambra1b , nucleoporin 62 ( p62 ), beclin1 , bcl2a , bcl2b , baxa , baxb , caspase3a , caspase9, p53 , and forkhead transcription factor 3a ( fox3a ) 32,33 .…”

Section: Resultsmentioning

confidence: 99%

Fundc1 is necessary for proper body axis formation during embryogenesis in zebrafish

Shen

Nibona

et al. 2019

Sci Rep

View full text Add to dashboard Cite

FUN14 domain-containing protein 1 (FUNDC1) is a mitochondrial outer membrane protein which is responsible for hypoxia-induced mitophagy in mammalian cells. Knockdown of fundc1 is known to cause severe defects in the body axis of a rare minnow. To understand the role of Fundc1 in embryogenesis, we used zebrafish in this study. We used bioimaging to locate zebrafish Fundc1 (DrFundc1) with MitoTracker, a marker of mitochondria, and/or CellLight Lysosomes-GFP, a label of lysosomes, in the transfected ovary cells of grass carp. The use of Western blotting detected DrFundc1 as a component of mitochondrial proteins with endogenous COX IV, LC3B, and FUNDC1 in transgenic human embryonic kidney 293 T cells. DrFundc1 induced LC3B activation. The ectopic expression of Drfundc1 caused cell death and apoptosis as well as impairing cell proliferation in the 293 T cell line, as detected by Trypan blue, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and incorporation of BrdU. DrFundc1 up-regulated expression of both autophagy- and apoptosis-related genes, including ATG5, ATG7, LC3B, BECLIN1, and BAX in transgenic 293 T cells. A knockdown of Drfundc1 using short hairpin RNA (shRNA) led to midline bifurcation with two notochords and two spinal cords in zebrafish embryos. Co-injection of Drfundc1 mRNA repaired defects resulting from shRNA. Knockdown of Drfundc1 resulted in up- or down-regulation of genes related to autophagy and apoptosis, as well as decreased expression of neural genes such as cyclinD1, pax2a, opl, and neuroD1. In summary, DrFundc1 is a mitochondrial protein which is involved in mitophagy and is critical for typical body axis development in zebrafish.

show abstract

Beneficial Bacteria Affect Danio rerio Development by the Modulation of Maternal Factors Involved in Autophagic, Apoptotic and Dorsalizing Processes

Cited by 22 publications

References 67 publications

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

The Role of Probiotics in Lipopolysaccharide-Induced Autophagy in Intestinal Epithelial Cells

Fundc1 is necessary for proper body axis formation during embryogenesis in zebrafish

Contact Info

Product

Resources

About